Construction method of animal models of cognitive decline caused by anxiety combined with continuous sleep loss, and application of animal models

An animal model and construction method technology, which can be used in compound screening/testing, pharmaceutical formulations, preparations for in vivo testing, etc. The effect of knowledge ability, simple operation and short modeling cycle

Inactive Publication Date: 2020-03-24
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings and shortcomings of the existing animal models that cannot pay attention to the cognitive decline caused by sleep loss and anxiety at the same time, and the existing construction methods have long modeling period and invasiveness, and provide an anxiety joint A method for constructing an animal model of cognitive decline caused by continuous sleep loss. This method also pays attention to the cognitive decline caused by anxiety and sleep loss, and has the advantages of short cycle, low cost, simple operation, non-invasiveness, high degree of scene simulation, The advantages of good modeling effect

Method used

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  • Construction method of animal models of cognitive decline caused by anxiety combined with continuous sleep loss, and application of animal models
  • Construction method of animal models of cognitive decline caused by anxiety combined with continuous sleep loss, and application of animal models
  • Construction method of animal models of cognitive decline caused by anxiety combined with continuous sleep loss, and application of animal models

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Experimental program
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Effect test

Embodiment 1

[0035] 1. Construction of an animal model of cognitive decline caused by anxiety combined with continuous sleep loss

[0036] Eight male Kunming mice weighing 18-22 g were purchased from Guangdong Medical Experimental Animal Center. They were adaptively fed for 3 days before the experiment. Divide them into blank control group and model group according to body weight, with 4 mice in each group, and mark them well, so that there is no significant difference in weight of mice in each group.

[0037] The experimental modeling period is two days. During the modeling period, the experimental parameters of noise, electrical stimulation and light interference (hereinafter referred to as acousto-optic) were set as the following data: noise interference was performed by using an electric bell for noise stimulation (120dB), and the cycle frequency was 1min / 0.5h; electrical stimulation was performed by 53- The 63V alternating current is connected to the self-made power grid for stimula...

Embodiment 2

[0052] 1. Construction of an animal model of cognitive decline caused by anxiety combined with continuous sleep loss

[0053] Eight male Kunming mice weighing 18-22 g were purchased from Guangdong Medical Experimental Animal Center. They were adaptively fed for 3 days before the experiment. Divide them into blank control group and model group according to body weight, with 4 mice in each group, and mark them well, so that there is no significant difference in weight of mice in each group.

[0054] The experimental modeling period is two days. During the modeling period, the experimental parameters of noise, electrical stimulation and light interference (hereinafter referred to as acousto-optic) were set as the following data: during noise interference, an electric bell with a diameter of 55mm was used for noise stimulation (120dB), and the cycle frequency was 1min / 0.5h; Stimulation is carried out by connecting a self-made grid with 20-30V alternating current, with a cycle fr...

Embodiment 3

[0068] 1. Construction of an animal model of cognitive decline caused by anxiety combined with continuous sleep loss

[0069] Eight male Kunming mice weighing 18-22 g were purchased from Guangdong Medical Experimental Animal Center. They were adaptively fed for 3 days before the experiment. Divide them into blank control group and model group according to body weight, with 4 mice in each group, and mark them well, so that there is no significant difference in weight of mice in each group.

[0070] The experimental modeling period is two days. During the modeling period, the experimental parameters of noise, electrical stimulation and light interference (hereinafter referred to as acousto-optic) are set as the following data: noise interference is performed by using an electric bell for noise stimulation (60dB), and the cycle frequency is 1min / 2h; electrical stimulation is controlled by 53-63V The alternating current is connected to the self-made power grid for stimulation, a...

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Abstract

The invention discloses a construction method of animal models of cognitive decline caused by anxiety combined with continuous sleep loss as well as application of the animal models. The constructionmethod of the animal models of cognitive decline caused by anxiety combined with continuous sleep loss comprises the following steps: stimulating experimental animals by performing combined noise intervention, electrical stimulation intervention and light interference intervention; and then, performing animal behavior tests on the experimental animals after completion of the interventions. The construction method of the animal models of cognitive decline caused by anxiety combined with continuous sleep loss disclosed by the invention focuses cognitive decline synchronously caused by anxiety combined with continuous sleep loss; and moreover, the construction method is easy to operate, short in modeling cycle, good in modeling effects, and non-invasive to the animals. The animal models obtained by the construction method can be closer to scenarios of health symptoms of target populations with cognitive decline caused by the status of anxiety and continuous sleep loss due to high pressure, heavy workload and emotional tension; and thus, the animal models can be used for effective evaluation on lifestyle, diet and drugs on symptoms of cognitive decline caused by anxiety combined with continuous sleep loss. Moreover, reliable evaluation models are also provided for screening of related foods and health products, drug research and development, and the like.

Description

technical field [0001] The invention belongs to the technical field of animal model construction. More specifically, it relates to a method for constructing an animal model of cognitive decline caused by anxiety combined with continuous sleep loss and its application. Background technique [0002] Due to the rapid development of science and technology in modern society, the work is becoming increasingly tense, the pace of life is accelerating, and the pressure in fierce competition is increasing, making more and more people in a state of anxiety and sleep deprivation for a long time, especially mental workers and school students why. Being in a state of anxiety for a long time will increase the possibility of anxiety and depression; long-term lack of sleep will bring many physical and mental injuries, such as decreased thinking ability, weakened alertness and judgment, and immune dysfunction. Anxiety state and lack of sleep are related to each other and promote each other,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A01K2227/105A01K2267/0356A61K49/0008
Inventor 钟倩霞周勇丁刘刚杜志云潘春杏李宜鸣
Owner INFINITUS (CHINA) CO LTD
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