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A method of artificial oxytocin-natural mating and fertilization-embryo floating water hatching

A technology of artificial induced spawning and natural mating, applied in the direction of climate change adaptation, application, fish farming, etc., can solve problems such as difficult to collect high-efficiency hatching, easy to be eaten by big fish, and affect the production efficiency of artificial breeding, so as to avoid fertilization Effect of loss of eggs and fry, easy hanging and transfer, low production cost

Active Publication Date: 2021-02-02
HUNAN NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Due to the stickiness of sticky fish eggs, the fertilized eggs often adhere to the inner wall of the pool randomly, and are easily eaten by big fish, and are difficult to collect and transfer to special hatching tanks for high-efficiency incubation, which affects the production efficiency of artificial breeding

Method used

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  • A method of artificial oxytocin-natural mating and fertilization-embryo floating water hatching
  • A method of artificial oxytocin-natural mating and fertilization-embryo floating water hatching
  • A method of artificial oxytocin-natural mating and fertilization-embryo floating water hatching

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Embodiment

[0038] A method of artificial oxytocin of the present invention-natural mating and fertilization-embryo buoyant flowing water hatching comprises the following steps:

[0039] (1) Artificial induction: During the 2018 breeding season, we fished with nets, selected mature gonads (female parent fish with soft and swollen abdomen and male parent fish that can lightly squeeze out milky white semen), and induced labor on them. Inject the mixed oxytocic agent of luteinizing hormone releasing hormone analog (9ug / kg) and chorionic gonadotropin (500IU / kg) to the root to induce labor, and the needle is directed towards the abdomen, and intraperitoneal injection is carried out. In the gills, inject the male parent fish first, and then inject the female parent fish after 3 hours;

[0040] (2) Natural mating and fertilization: the female parent fish and the male parent parent fish after the artificial induced labor are put into the same induced labor net cage in the round pond, and the fert...

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Abstract

The invention discloses a method for fish artificial oxytocin-natural mating and fertilization-embryo floating water hatching, the method comprising the following steps: 1) injecting oxytocin into broodstock with well-developed gonads for artificial oxytocin; 2) injecting oxytocin The parent fish is put into the net cage that has attachment, treats that it is in estrus and rear-ends, and fertilizes on attachment naturally; Compared with traditional artificial insemination and hatching, the present invention significantly improves the hatching rate of fish fertilized eggs and the survival rate after entering the pond, reduces the loss of broodstock, reduces labor costs, and is suitable for continuous hatching and breeding of sticky fish eggs, which is beneficial to Improve the production efficiency of fish artificial breeding. The invention provides a new model for the genetic breeding of fish, and has great significance in the genetic breeding of fish.

Description

technical field [0001] The invention belongs to the technical field of fish reproduction, and in particular relates to a method for artificial induced labor-natural mating and fertilization-embryo floating water hatching. Background technique [0002] There are more than 32,000 species of fish in nature, which is the most diverse among vertebrates. Fish is rich in protein, vitamins and essential minerals, and also contains a large amount of essential amino acids, which is an important protein source for human beings. Most of these proteins come from artificial breeding, and the artificial breeding of fish is a crucial part of this. [0003] At present, artificial breeding of fish is mostly the traditional method of artificial oxytocin-artificial dry / wet insemination-hatching. The estrus time of parent fish after induced labor is closely related to the maturity of fish gonads, the dose of oxytocin, and the water temperature. The estrus time is uncertain; it is often necessa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/17
CPCA01K61/17Y02A40/81
Inventor 刘少军吴昌罗凯坤廖志元张纯陶敏覃钦博汤陈宸王余德胡方舟王石刘庆峰
Owner HUNAN NORMAL UNIVERSITY
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