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Method for producing strains by extracting original strains from glossy ganoderma to convert into liquid

A technology of liquid strains and Ganoderma lucidum, which is applied in the field of transforming the original strains of Ganoderma lucidum into liquid production strains, can solve the problems of low production capacity of Ganoderma lucidum polysaccharides, and achieve the effects of centralized seed production, reduced equipment requirements, and small volume

Pending Publication Date: 2020-03-27
石家庄亚特生物科技有限公司
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Problems solved by technology

[0003] The purpose of the embodiments of the present invention is to provide a method for transforming the original strains of ganoderma into liquid production strains to solve the problem of low production capacity of ganoderma lucidum polysaccharides

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  • Method for producing strains by extracting original strains from glossy ganoderma to convert into liquid

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Embodiment 1

[0016] see figure 1 , the technical scheme of the method for extracting original strains of ganoderma to transform liquid production strains provided by the invention: the method for extracting original strains of ganoderma to transform liquid production strains, the ganoderma strains selected for testing are black ganoderma and purple ganoderma, Inoculate each Ganoderma lucidum strain on PDA medium, culture at 25°C for 10 days, and store at 4°C. The preparation method of the strain includes the following steps: Step 1: The activation of the strain is to cut out a 0.5cm Mycelial pieces of different sizes were inoculated in the middle of the slant medium, cultured for 7 days, then transferred once, and cultured at 25°C for 7 days before use;

[0017] Step 2: For liquid culture, the first is the preparation of the seed liquid. Take 2 to 3 pieces of activated bacteria with a size of 1 em in the basal medium, place them on a rotary shaker at 25°C and shake them at 180r / min for 7 d...

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Abstract

Embodiments of the invention disclose a method for producing strains by extracting original strains from glossy ganoderma to convert into liquid. Ganoderma atrum and ganoderma sinense are selected asganoderma strains for test; and each ganoderma strain is inoculated on a PDA medium to culture for 10 d at 25 DEG C and preserve at 4 DEG C. A preparation method of the strains includes the followingsteps: step one, the step of strain activation; step two, the step of liquid culture; step three, the step of fermentation tank culture, a fermentation tank culture medium selecting an optimal polysaccharide-producing medium through orthogonal test. A complex and strict liquid fermentation process can be converted into a solid cultivation process through the strains preparing liquid, so that the method is simpler and more reliable, and requirements for equipment can be greatly reduced; and the concentrated strains are small in volume after homogenate, so that long-distance transportation of the strains under refrigeration can be realized, and centralized strain producing can be achieved.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of strain application, and specifically relates to a method for extracting ganoderma lucidum from original strains and transforming them into liquid production strains. Background technique [0002] At present, Ganoderma lucidum polysaccharides with anti-tumor effects are mainly extracted from fruiting bodies, but the production of fruiting bodies is greatly affected by natural environmental factors, which affects the development and production of Ganoderma lucidum polysaccharides; and the use of liquid submerged fermentation has short cycle, simple process and low cost. , large output, suitable for factory production and other significant advantages, the method greatly improves the production capacity of Ganoderma lucidum polysaccharide. Contents of the invention [0003] The purpose of the embodiment of the present invention is to provide a method for transforming the original s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 贾彦军
Owner 石家庄亚特生物科技有限公司
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