Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of sandwich type photoelectrochemical sensor for detecting prostate specific antigen

A prostate-specific and prostate antigen-based technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problem of reduced photocurrent intensity, achieve the effects of reduced photocurrent, good reproducibility, and fast detection

Active Publication Date: 2020-03-27
SHANDONG UNIV OF TECH
View PDF10 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The energy transfer (ET) process occurs simultaneously, and the photocurrent intensity will decrease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 prepares sea urchin shape titanium dioxide and comprises:

[0044] Embodiment 1 prepares sea urchin shape titanium dioxide, the steps are as follows:

[0045] Add 0.5 mL of tetrabutyl titanate into an Erlenmeyer flask containing 20 mL of glacial acetic acid, and keep stirring at room temperature. After 10 min, transfer the milky white suspension to a polytetrafluoroethylene-lined reaction kettle at 130 °C After reacting for 12 h and cooling down to room temperature, the white product was collected, washed several times with ultrapure water and absolute ethanol, and dried under reduced pressure at 60 °C to obtain TiO 2 .

Embodiment 2

[0046] Embodiment 2 prepares sea urchin-shaped titanium dioxide, the steps are as follows:

[0047] Add 1 mL of tetrabutyl titanate into an Erlenmeyer flask containing 30 mL of glacial acetic acid, and keep stirring at room temperature. After 10 min, transfer the milky white suspension to a polytetrafluoroethylene-lined reaction kettle, 140 ℃ for 12 h, lowered to room temperature, collected the white product, washed several times with ultrapure water and absolute ethanol, and dried under reduced pressure at 60 ℃ to obtain TiO 2 .

Embodiment 3

[0048] Embodiment 3 prepares sea urchin-shaped titanium dioxide, the steps are as follows:

[0049] Add 2 mL of tetrabutyl titanate into an Erlenmeyer flask containing 40 mL of glacial acetic acid, and keep stirring at room temperature. After 10 min, transfer the milky white suspension to a polytetrafluoroethylene-lined reaction kettle. ℃ for 12 h, lowered to room temperature, collected the white product, washed several times with ultrapure water and absolute ethanol, and dried under reduced pressure at 60 ℃ to obtain TiO 2 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method and application of a sandwich type photoelectrochemical sensor for detecting a prostate specific antigen (PSA). Nitrogen-doped graphene quantum dots (N-GQDs) and cadmium sulfide (CdS) quantum dots double-sensitized sea urchin-shaped titanium dioxide (TiO2) are used as photoactive materials to be immobilized on the surface of the conductive glass. The compound of electron holes (e<-> / h<+>) is effectively inhibited, a remarkable photocurrent signal is generated, and rich functional groups are provided for immobilizing PSA antibodies. A gold-carbon nanotube (Au / MWCNTs) nano-composite is adopted as a label of a second antibody, and exciton-plasma resonance (EPI) is generated according to different mechanisms. In the system, Au NPs are enabled to generate surface plasma resonance (SPR) in an optical excitation state. Along with the energy resonance transfer (ET) process, the photocurrent intensity is reduced to a certain extent, and the sensitivity of the sensor is improved. In addition, Au / MWCNTs have a large surface area and biocompatibility. The loading capacity of the second antibody is increased, due to the fact that the steric hindrance effect hinders electron transfer, the photocurrent is reduced, the sensitivity of the sensor is further improved, specific detection of the prostate specific antigen is achieved, a novel and feasible detection method is provided for early detection of PSA, and the prostate specific antigen sensor has potential application prospects in clinic.

Description

technical field [0001] The invention designs the technical fields of bioanalytical chemistry, nanomaterials, immunoassay and photoelectrochemical biosensing, and provides a preparation method and application of a sandwich-type photoelectrochemical sensor for detecting prostate specific antigen. Urchin-like TiO was synthesized by hydrothermal method 2 , N-GQDs and CdS QDs as dual sensitizers and antibody capture materials immobilized on TiO 2 On the surface, using Au / MWCNTs nanocomposites with dual inhibition as secondary antibody markers, a preparation method and application of a sandwich-type photoelectrochemical sensor for detecting prostate specific antigen are provided. Background technique [0002] Prostate cancer has become the second most common cancer among men worldwide and a common cause of death among men in every country. Prostate-specific antigen (PSA), an intracellular glycoprotein (34 kDa, kallikrein-like protease), is considered as a major tumor marker for ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N33/541G01N33/543
CPCG01N27/48G01N33/541G01N33/54346
Inventor 孔玲孙晓凯陈志伟李灿国朱琪颖陈杰魏萌刘东武李月云
Owner SHANDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com