Application of reaction of microbial bacterial liquid and serum vesicles in detection of microbial infection

A microbial liquid and microbial infection technology, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of the influence of the patient's condition, the low reliability of bacterial culture results, and the long time required for microbial culture. The test results are reliable and can guide the effect of clinical medication

Pending Publication Date: 2020-03-31
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

[0004] In the existing diagnostic methods, for the culture and identification of microorganisms, the low culture rate has also become one of the important factors affecting its development. In order to improve the positive rate of infected tissue culture during surgery, it is not recommended to routinely use antibiotics before surgery treat
However, for patients with clear sinuses that communicate with the outside world, the reliability of bacterial culture results is low due to the presence of external pollution factors.
In addition, microbial culture takes a long time, and antibiotics need to be used empirically during this period, which will have a certain impact on the treatment of patients' conditions
For PCR technology, because it is still in the exploratory stage, and it has certain defects, such as specimen contamination can easily lead to false positives, the survival status of bacteria cannot be identified, and the drug susceptibility information provided is limited, etc., it is not recommended to use this technology as a microbial identification routine testing methods
[0005] In the prior art, there is a lack of a fast, effective and highly reliable microbial infection detection technology

Method used

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  • Application of reaction of microbial bacterial liquid and serum vesicles in detection of microbial infection
  • Application of reaction of microbial bacterial liquid and serum vesicles in detection of microbial infection
  • Application of reaction of microbial bacterial liquid and serum vesicles in detection of microbial infection

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Embodiment Construction

[0028] Application of serum vesicles from infected individuals in the preparation of corresponding microbial infection detection kits.

[0029] In the research, the inventor unexpectedly discovered that the patient's serum extracts, especially the serum vesicles, will produce an agglutination reaction with the bacterial solution. By detecting the number and type of agglutination, the type of microbial infection can be determined conveniently, quickly and accurately. For example, compared with other microorganisms, in patients infected with Staphylococcus aureus, the reaction of serum vesicles with Staphylococcus aureus liquid will produce a significantly more obvious agglutination reaction, through the agglutination with a particle size larger than a certain threshold (such as 3 μm) The number can conveniently and quickly determine the type of microbial infection, and then better guide clinical medication.

[0030] The application of the microbial bacterial liquid in the prepa...

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Abstract

The invention discloses an application of a reaction of a microbial bacterial liquid and serum vesicles in detection of microbial infection. The inventors unexpectedly discover in the study that the patient serum extract and the bacterial liquid can be subjected to an agglutination reaction, by detecting the number and type of agglutination blocks, the type of the microbial infection can be determined conveniently, quickly and accurately. Through some embodiments of the invention, the microbial infection can be conveniently and quickly detected, the detection results are reliable, and clinicalmedication can be better guided.

Description

technical field [0001] The invention relates to the field of detection of microbial infection, in particular to the application of the reaction between microbial liquid and serum vesicles in the detection of microbial infection. Background technique [0002] Infection after internal fixation of fractures is one of the clinical problems faced by orthopedic surgeons. The reason is that its diagnosis is often difficult and its treatment is relatively difficult. Once the infection cannot be effectively controlled in time, chronic osteomyelitis is very easy to occur, resulting in prolonged infection and high recurrence rate. High, high disability rate. At the same time, infection after internal fixation of fractures is also catastrophic, bringing heavy social and economic burdens to patients and their families. Early accurate diagnosis and reasonable and standardized treatment are the keys to increase the cure rate, reduce the recurrence rate and disability rate, restore limb fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 余斌张先荣邓淞云刘冠峤
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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