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Method for extracting natural effective components of bacteriostatic agent

A natural antibacterial agent and active ingredient technology, applied in the field of plant extraction, can solve the problems of difficult to handle organic solvents, complex processes, complex components, etc., and achieve the effect of improving the extraction degree, good antibacterial effect, and strong adsorption capacity

Inactive Publication Date: 2020-04-03
WEST ANHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the complexity of the components in the natural product extract, obtaining the target components by a single membrane separation technology or adsorption separation technology often fails to meet the requirements. Therefore, other separation technologies have to be used, such as precipitation, salting out, and extraction. , crystallization and recrystallization, etc., the process is complex, especially the addition of organic solvents is difficult to handle, and the cost is high

Method used

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  • Method for extracting natural effective components of bacteriostatic agent
  • Method for extracting natural effective components of bacteriostatic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting natural bacteriostatic active ingredients, comprising the steps of:

[0028] S1. Put 1 kg of mugwort leaves and 1 kg of honeysuckle on the grid of the steam kettle, and steam from the bottom of the steam kettle to heat the leaves and honeysuckle for 10 minutes. After taking them out, quickly put them into the freezer, keep them at -20°C for 10 minutes, and crush them in a frozen state. The particle size is 100-200 μm, thawed to room temperature, heated again in a steam kettle for 20 minutes, quickly put it into a freezer kettle after taking it out, kept it at -30°C for 6 minutes, continued to crush it in a frozen state, passed through a 160-mesh sieve, and thawed it to room temperature to obtain prefabricated material;

[0029] S2. Add water to 100kg of prefabricated material until the water content is 50wt%, add 0.1kg of cellulase and stir evenly, keep warm at 30°C for 15h, inactivate at high temperature, set the temperature at 60°C, add 5kg of ...

Embodiment 2

[0032] A method for extracting natural bacteriostatic active ingredients, comprising the steps of:

[0033] S1. Put 0.5 kg hemp seed, 3 kg cortex cortex, and 2 kg dandelion on the grid of the steam kettle, pass steam from the bottom of the steam kettle to heat the hemp seed, cortex cortex and dandelion for 20 minutes, take them out and quickly put them into the freezer, - Incubate at 15°C for 20 minutes, crush until the particle size is 100-200 μm in a frozen state, thaw to room temperature, heat again in a steam kettle for 10 minutes, take it out and quickly put it in a freezing kettle, keep it at -40°C for 2 minutes, and continue crushing in a frozen state , passed through a 160-mesh sieve, and thawed to room temperature to obtain a prefabricated material;

[0034] S2. Add water to 100kg of prefabricated material until the water content is 60wt%, add 0.01kg of cellulase and stir evenly, keep warm at 36°C for 5h, inactivate at high temperature, set the temperature at 68°C, ad...

Embodiment 3

[0037] A method for extracting natural bacteriostatic active ingredients, comprising the steps of:

[0038]S1. Put 2kg cortex flavescens, 2kg flavescens, and 0.5kg tea leaves on the grid of the steam kettle, and steam from the bottom of the steam kettle to heat the cork cork, flavescens flavescens, and tea leaves for 13 minutes. After taking them out, quickly put them into the freezer at -18°C Insulate for 12 minutes, crush until the particle size is 100-200 μm in the frozen state, thaw to room temperature, heat again in the steam kettle for 16 minutes, take it out and quickly put it in the freezing kettle, keep it at -33°C for 5 minutes, continue crushing in the frozen state, 160 mesh sieve, thawed to room temperature to obtain prefabricated material;

[0039] S2. Add water to 100kg of prefabricated material until the water content is 52wt%, add 0.08kg of cellulase and stir evenly, keep warm at 32°C for 12h, inactivate at high temperature, set the temperature at 62°C, add 4kg...

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Abstract

The invention discloses a method for extracting natural effective components of a bacteriostatic agent. The method comprises the following steps: carrying out primary steam heating on natural plants,then carrying out primary freezing treatment, carrying out primary crushing in the primary freezing treatment process, unfreezing the crushed material to room temperature, carrying out secondary steamheating, then carrying out secondary freezing treatment, carrying out secondary crushing in the secondary freezing treatment process, and unfreezing obtained powder to room temperature to obtain a prefabricated material; adding water into the prefabricated material, adding cellulase for enzymolysis and inactivation, adding nano bentonite and nano aluminum oxide for mixing, then adding microcrystalline cellulose, performing stirring, adding a cross-linking agent in a stirring state, performing stirring, standing, concentrating and drying to obtain the natural effective components of the bacteriostatic agent. According to the invention, the natural components are extracted from plants, and have a good antibacterial effect. The natural components contain the natural plant extract with the antibacterial effect, so that the addition amount of original chemical preservatives can be reduced, and meanwhile, the product has antibacterial and anti-inflammatory effects.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting active ingredients of natural antibacterial agents. Background technique [0002] Bacteriostatic agents refer to substances that can keep the activity of microorganisms such as bacteria, fungi, algae, yeast and viruses at a low level within a certain period of time, inhibit their growth and reproduction, and prevent them from multiplying too much. Usually, bacteriostatic agents It can be divided into three categories: organic antibacterial agents, inorganic antibacterial agents and natural antibacterial agents. [0003] Bacteriostatic agents extracted from natural products often have low content of active ingredients and complex components, but the chemical composition of natural products is an important source for discovering new drugs or active lead compounds of drugs, and is also widely used in agriculture, industry, and daily chemical industry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3472A61K8/9749A61K8/9789A61Q19/00
CPCA23L3/3472A23V2002/00A61K8/9749A61K8/9789A61K2800/10A61K2800/524A61K2800/805A61K2800/84A61Q19/00A23V2250/21A23V2250/214
Inventor 汪学军闵长莉闵运江张莉殷智超
Owner WEST ANHUI UNIV
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