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Molecular marker for distinguishing papillary thyroid carcinoma from benign thyroid nodules

A papillary carcinoma and molecular marker technology, which is applied in the field of molecular markers and detection kits for distinguishing papillary thyroid carcinoma and benign thyroid nodules, and can solve problems such as the decreased expression level of LncRNAGAS8-AS1

Inactive Publication Date: 2020-04-07
BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

LncRNA GAS8-AS1 is the transcription product of the second intron of the growth arrest specific gene (growth arrest specific 8, GAS8), with a length of 994 nt.

Method used

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  • Molecular marker for distinguishing papillary thyroid carcinoma from benign thyroid nodules
  • Molecular marker for distinguishing papillary thyroid carcinoma from benign thyroid nodules
  • Molecular marker for distinguishing papillary thyroid carcinoma from benign thyroid nodules

Examples

Experimental program
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Embodiment 1

[0025] Example 1. Detection of RNA sequence fragments of GAS8-AS1 in the target population

[0026] 1. Take whole blood from the patient on an empty stomach, and centrifuge to obtain 2ml of plasma;

[0027] 2. Use TRIZOL (Invitrogen life technologies) method to extract total RNA in plasma;

[0028] 3. Through SuperScriptTM III Reverse Transcriptase (Invitrogen) and 2.5mM dNTP mixture (dATP, dGTP, dCTP and dTTP each 2.5mM) (HyTest Ltd) Primer (Yingjun Biotechnology Co., Ltd.) in Gene Amp PCR System 9700 (AppliedBiosystems) Reverse transcription reaction was carried out to obtain cDNA;

[0029] 4. Using 2X PCR master mix (Arraystar) kit, prepare the following reaction system: 2×MasterMix 5μl, 10uM primer F 0.5μl, heteroprimer R 0.5μl, cDNA 2ul, add water to a total volume of 10μl, and the above reaction system The PCR reaction was performed on a Realtime PCR (ViiA 7 Real-timePCR System, Applied Biosystems) instrument. The primers for each gene were designed as follows, the ex...

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Abstract

The invention relates to a molecular marker for distinguishing papillary thyroid carcinoma from benign thyroid nodules. The molecular marker is an RNA sequence segment of GAS8-AS1, wherein the RNA segment is as shown in SEQ ID No. 2, and a coding DNA sequence of the RNA sequence segment is as shown in Seq ID No.1. The invention also relates to application of the RNA fragment in preparation of a detection reagent or kit for distinguishing papillary thyroid carcinoma from benign thyroid nodules.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically relates to a molecular marker and a detection kit for distinguishing thyroid papillary carcinoma and thyroid benign nodules. Background technique [0002] Thyroid cancer is a common malignant tumor of the endocrine system, and its incidence continues to rise worldwide. In the past 10 years in the United States, the incidence of thyroid cancer has increased by 209%. In China, the incidence of thyroid cancer has surpassed endometrial cancer and ovarian cancer among women, becoming the top ten cancers with high incidence. The rapid growth rate is worrying. Thyroid cancer can be divided into papillary thyroid carcinoma (PTC), follicular carcinoma, medullary carcinoma, and undifferentiated carcinoma according to pathological types, among which papillary thyroid carcinoma is the most common, accounting for about 80%. Although the prognosis of most PTCs is good, a small nu...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6883C12N15/113
CPCC12Q1/6886C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 张冬雪姜涛
Owner BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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