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Chip for detecting nine gastric cancer and gastritis serum markers and preparation method thereof

A diagnostic marker and chip technology, applied in the biological field, can solve the problems of high cost, low efficiency, cumbersome operation, etc., and achieve the effect of high detection rate, simple detection, and good performance

Pending Publication Date: 2020-04-14
江苏三联生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of low efficiency, cumbersome operation and high cost in the original technology, the applicant of the present invention provides a chip for one-time detection of nine gastric cancer and gastritis serum markers and its preparation method

Method used

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  • Chip for detecting nine gastric cancer and gastritis serum markers and preparation method thereof

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Effect test

Embodiment 1

[0032] A chip for detecting nine gastric cancer and gastritis serum markers, the chip is prepared through the following steps:

[0033] (1) Black slide pretreatment;

[0034] ① Soak the black slide in the slide pretreatment solution containing NaOH for 16 hours, and then wash it with purified water for 2 to 8 times;

[0035] ② Soak the black glass slide in a silane solution with a mass concentration of 1 wt% (the medium is 25% ethanol) for 20 min;

[0036] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 180°C for 0.2h.

[0037] (2) Spotting capture antibody / specific antigen component solution;

[0038] refer to figure 1 , using automatic machine spotting, dotted into a 4×12 matrix on a black slide; each column is a dot formed by the same capture antibody / specific antigen component solution; the capture antibody / specific antigen component solution includes PGⅠ capture Antibody, PGⅡ capture antibody, G17 capture antibody, HP anti...

Embodiment 2

[0044] A chip for detecting nine gastric cancer and gastritis serum markers, the chip is prepared through the following steps:

[0045] (1) Black slide pretreatment;

[0046] ① Soak the black slide in the slide pretreatment solution containing NaOH for 24 hours, and then wash it with purified water for 2 to 8 times;

[0047] ② Soak the black glass slide in a silane solution with a mass concentration of 0.05% by weight (the medium is 25% ethanol) for 60 minutes;

[0048] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 140°C for 0.4h.

[0049] (2) Spotting specific component solutions;

[0050] refer to figure 1 , using automatic machine spotting, dotted into a 4×12 matrix on a black slide; each column is a dot formed by the same capture antibody / specific antigen component solution; the capture antibody / specific antigen component solution includes PGⅠ capture Antibody, PGⅡ capture antibody, G17 capture antibody, HP antigen, Ghrel...

Embodiment 3

[0056] A chip for detecting nine gastric cancer and gastritis serum markers, the chip is prepared through the following steps:

[0057] (1) Black slide pretreatment;

[0058] ① Soak the black slide in the slide pretreatment solution containing NaOH for 20 hours, and then wash it with purified water for 2 to 8 times;

[0059] ② Soak the black glass slide in a 0.5wt% silane solution (medium: 25% ethanol) for 30 minutes;

[0060] ③Purge the soaked black glass slides with nitrogen gas, put them into an oven, and bake them at 100°C for 0.6h.

[0061] (2) Spotting capture antibody / specific antigen component solution;

[0062] refer to figure 1 , use machine automatic spotting, spot on the black slide into a matrix of 4×12; each column is the spot formed by the same capture antibody / specific antigen component solution; each column is the spot formed by the same specific antigen component solution.

[0063] (3) blocking capture antibody / specific antigen component solution;

[006...

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Abstract

The invention discloses a protein chip capable of simultaneously detecting concentrations of nine gastric cancer and gastritis serum markers. The chip is prepared by the following steps: (1), pretreating a black slide; (2), performing sample application to capture an antibody / specific antigen component solution; and (3), sealing the chip to obtain the chip. The chip is used for detecting the concentrations of gastric cancer and gastritis serum markers in blood of a patient, and a guiding basis is provided for clinical gastritis and gastric cancer differential diagnosis. The kit formed by the method is capable of realizing early, rapid and accurate diagnosis of gastritis and gastric cancer as well as realizing full-automatic operation of gastric cancer and gastritis serum marker detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chip for detecting the concentrations of nine gastric cancer and gastritis serum markers and a preparation method thereof. Background technique [0002] The stomach is one of the important digestive organs in the human body and can be divided into cardia, fundus, body, antrum and pylorus. Under normal circumstances, the stomach has four main functions: food storage, digestion and absorption, secretion and defense. When the stomach is damaged and its function is disordered, stomach disease will occur, which will bring great harm to the human body. Stomach disease is a general term for many diseases related to the stomach [1,2] . Clinically common stomach diseases mainly include gastritis and benign and malignant tumors of the stomach. Among them, gastritis is an inflammatory reaction in which the inner wall of the stomach loses protection due to various reasons and is stimulated ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N33/552G01N33/535
CPCG01N33/535G01N33/552G01N33/57446G01N33/6893G01N2800/062G01N2800/52
Inventor 成舜泮锋纲丁俊杰施启尧
Owner 江苏三联生物工程股份有限公司
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