Preparation of mimic oxidase and application of mimic oxidase in photocatalysis bacteriostasis and sterilization

A technology of oxidase and photocatalysis, which is applied in the direction of physical/chemical process catalysts, applications, active ingredients of peroxides, etc., to achieve environmental protection, biocompatibility, obvious antibacterial and bactericidal effects, and increased permeability

Inactive Publication Date: 2020-04-17
云南伦扬科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of carbon dots as nanozymes is currently mainly used in the detection field, such as glucose,...

Method used

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  • Preparation of mimic oxidase and application of mimic oxidase in photocatalysis bacteriostasis and sterilization
  • Preparation of mimic oxidase and application of mimic oxidase in photocatalysis bacteriostasis and sterilization
  • Preparation of mimic oxidase and application of mimic oxidase in photocatalysis bacteriostasis and sterilization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of nitrogen- and iodine-doped carbon dots (N / I-CDs) simulated oxidase: Weigh 2 parts of iopromide, add 120 parts of ultra-pure water, mix and dissolve, add 0.5 part of ethylenediamine, and sonicate for 15 minutes , and transferred to a polytetrafluoroethylene reactor, heated at 180 ° C for 10 h, after natural cooling, first filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3000 D for 24 h to obtain water-soluble iodine nitrogen Doped with carbon dots, it is a simulated oxidase.

[0022] 2. Antibacterial and bactericidal effect of N / I-CDs simulated oxidase system on Escherichia coli:

[0023] (1) Culture of strains: Escherichia coli ( E. coli ATCC 25922) was purchased from the Institute of Microbiology, Yunnan University. The activated cells were inoculated into 5 mL of LB medium at an inoculum size of 2%, and cultured with shaking at 37 °C for 12 h. Dilute to 10 with 0...

Embodiment 2

[0031] 1. Preparation of nitrogen- and iodine-doped carbon dots (N / I-CDs) simulated oxidase: Weigh 1 part of iopromide, add 100 parts of ultra-pure water, mix and dissolve, add 0.3 part of ethylenediamine, and sonicate for 10 minutes , and transferred to a polytetrafluoroethylene reactor, heated at 180°C for 8h, and after natural cooling, first filtered with a filter membrane with a pore size of 0.22μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3000D for 24h to obtain water-soluble iodine nitrogen Doped with carbon dots, it is a simulated oxidase.

[0032] 2. Antibacterial and bactericidal effect of N / I-CDs simulated oxidase system Salmonella:

[0033](1) Culture of strains: Salmonella (ATCC14028) was purchased from the Institute of Microbiology, Yunnan University. The activated cells were inoculated into 5 mL of LB medium at an inoculum size of 2%, and cultured with shaking at 37 °C for 12 h. Dilute to 10 with 0.85% normal saline -1 、10 -2 、...

Embodiment 3

[0039] 1. Preparation of nitrogen- and iodine-doped carbon dots (N / I-CDs) simulated oxidase: Weigh 3 parts of iopromide, add 150 parts of ultra-pure water, mix and dissolve, add 1 part of ethylenediamine, and sonicate for 20 minutes , and transferred to a polytetrafluoroethylene reaction kettle, heated at 180 ° C for 12 h, after natural cooling, first filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3000D for 24 hours to obtain water-soluble iodine Nitrogen-doped carbon dots are simulated oxidases.

[0040] 2. Antibacterial and bactericidal effect of N / I-CDs simulated oxidase system on Staphylococcus aureus:

[0041] (1) Culture of strains: Staphylococcus aureus (ATCC25923) was purchased from the Institute of Microbiology, Yunnan University. The activated cells were inoculated into 5 mL of LB medium at an inoculum size of 2%, and cultured with shaking at 37 °C for 12 h. Dilute to 10 with 0.8...

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Abstract

The invention belongs to the technical field of preparation of nano materials, and particularly relates to preparation of a mimic oxidase and application of the mimic oxidase in photocatalysis, bacteriostasis and sterilization. According to the mimic oxidase, iopromide and ethylenediamine are used as raw materials; nitrogen-doped and iodine-doped carbon dots are synthesized by a one-step hydrothermal method; the enzyme characteristics are simulated based on synthetic carbon dots; the nitrogen-doped and iodine-doped carbon dots are mixed with hydrogen peroxide to form a novel antibacterial agent; coexistence of carbon dot nano-enzyme and hydrogen peroxide is utilized; under visible light irradiation, the oxidation activity of the nano-enzyme can promote the oxidation process of bacterial cells and the consumption of antioxidant biomolecules; the antibacterial activity of oxygen products including H2O2 and other accumulated antibacterial activities from reactive oxygen species (ROS) is reduced, a good antibacterial effect is generated, and due to the characteristics of safety, good biocompatibility and the like of the developed novel antibacterial agent, a great application prospectis achieved.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation, and in particular relates to the preparation of a simulated oxidase and its photocatalytic antibacterial and antibacterial application. Background technique [0002] With the increasingly serious problem of bacterial drug resistance and the increasing number of multi-drug resistant bacterial infections, bacterial drug resistance has become a thorny problem in the treatment of clinical diseases. The antibacterial effect of antibiotics has been widely recognized, but the continuous emergence and rapid spread of antibiotic-resistant bacteria has become one of the most serious threats to human health worldwide, and the research on new antibacterial agents has become a hot spot. The development of nanomaterial technology provides an opportunity for the development of new antibacterial agents. Due to the diversity of the mechanism of action on bacteria, bacterial cells are less likely ...

Claims

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Application Information

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IPC IPC(8): A01N59/00A01N59/12A61K33/40A61K41/00A61P31/04B01J21/18B01J27/24C01B32/15B82Y40/00B82Y20/00A01P1/00
CPCA01N59/00A01N59/12A61K33/40A61K41/0057A61P31/04B01J21/18B01J27/24B01J35/004B82Y20/00B82Y40/00C01B32/15A61K2300/00Y02A50/30
Inventor 陈子昭杨亚玲
Owner 云南伦扬科技有限公司
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