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A g-6-pd detection kit and its detection method not affected by anemia

A G-6-PD, kit technology, applied in the direction of material analysis, measuring device, instrument, etc. by observing the influence on chemical indicator, can solve the problem of inaccurate test results, achieve flexible and convenient operation, save manpower The effect of material resources and low cost

Active Publication Date: 2022-02-01
QINGDAO HIGHTOP BIOTECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of inaccurate detection results of anemia patients in the prior art, and propose a G-6-PD detection kit that can conveniently measure the degree of anemia of the subject and quickly confirm the amount of sample added and its The detection method is used for the screening detection of red blood cell glucose-6-phosphate dehydrogenase (G-6-PD) deficiency. The detection kit can compensate the sample amount according to the hemoglobin test result. Flexible and convenient, while improving the accuracy of test results

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  • A g-6-pd detection kit and its detection method not affected by anemia

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Embodiment

[0027] Preparation of G-6-PD test paper: soak the reaction film in the G-6-PD detection solution to saturation, cut it into shape after drying to obtain the G-6-PD detection block, and fix the G-6-PD detection block Paste on one end of the carrier to make a test paper;

[0028] Preparation of G-6-PD detection solution: 0.20mol / L Tris-HCl buffer solution (pH7.4); 0.15% phenazine dimethyl sulfate (M-PMS); 0.75% nitro blue tetrazolium (NBT); Glucose-6-phosphate disodium (G6P-Na 2 ); 12mmol / L Coenzyme II (NADP); 0.6mol / L MgCl 2 Mix in proportion to obtain G-6-PD detection solution.

[0029] Preparation of hemoglobin test paper: soak the reaction membrane in the hemoglobin detection solution to saturation, cut it into shape after drying to obtain a hemoglobin detection block, fix and paste the hemoglobin detection block on one end of the carrier, and prepare the test paper;

[0030] Preparation of hemoglobin detection solution: 0.1mol / L PBS buffer solution (pH6.5), 0.15% sodium ...

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Abstract

The invention belongs to the technical field of biochemical detection, and in particular relates to a G-6-PD detection kit which is not affected by anemia and a detection method thereof. , G-6-PD test paper and detection color comparison card; the sample detection device includes a sample tube, a first card slot and a second card slot. The kit and detection method provided by the present invention can conveniently measure the anemia degree of the subject, quickly confirm the sample amount, and be used for the screening detection of red blood cell glucose-6-phosphate dehydrogenase (G-6-PD) deficiency, and The amount of sample added can be compensated according to the hemoglobin test result, the test method uses less blood, is flexible and convenient to test, and improves the accuracy of the test result at the same time.

Description

technical field [0001] The invention belongs to the technical field of detection of in vitro diagnostic reagents, and in particular relates to a G-6-PD detection kit and a detection method thereof which are not affected by anemia. Background technique [0002] Anemia is a clinical syndrome in which the volume of peripheral blood red blood cells is lower than normal due to various reasons. (Hb) is most important. Chinese hematologists believe that in China's sea level areas, adult male Hb<120g / L (12.0g / dl), adult female Hb<110g / L (11.0g / dl), pregnant woman's Hb<120g / L, have anemia. Erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) is derived from erythrocytes, catalyzes glucose-6-phosphate, and the NAD-PH generated is the coenzyme of glutathione reductase, reduced glutathione (GSH ) is a necessary condition for maintaining the stability of hemoglobin and the integrity of the red blood cell membrane. In the absence of red blood cell G-6-PD, the function of th...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/01
CPCG01N21/78G01N21/01
Inventor 杨帆杨勤德邓旭崔欣顾晓云宋金玲刘秀艳
Owner QINGDAO HIGHTOP BIOTECH