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Grading model for detecting benign/malignant degrees of lymphoma and lymphatic metastatic cancer and application of grading model

A technology for lymphatic metastasis and lymphoma, applied in the field of gene diagnosis and biology, which can solve the problems of reducing recurrence rate, prolonging survival period, omission, etc.

Pending Publication Date: 2020-05-05
LISEN IMPRINTING DIAGNOSTICS (WUXI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, pathologically judging whether there are metastatic cancer cells in lymph nodes is usually based on morphological observation, but in the early stage of cancer metastasis, the number of cancer cells in lymph nodes is still small, and may be missed by morphological observation. Whether there is a technology for cancer cell metastasis can help doctors determine the extent of surgical resection and lymph node dissection, and know the choice of follow-up treatment options, which is of great significance to reducing the recurrence rate after surgery and prolonging survival

Method used

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  • Grading model for detecting benign/malignant degrees of lymphoma and lymphatic metastatic cancer and application of grading model
  • Grading model for detecting benign/malignant degrees of lymphoma and lymphatic metastatic cancer and application of grading model
  • Grading model for detecting benign/malignant degrees of lymphoma and lymphatic metastatic cancer and application of grading model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0159] Example 1 Imprinted gene analysis of malignant lymphoma

[0160] The detection method of the imprinted gene comprises the following steps:

[0161] (1) Obtain the tissue cell section (10 microns) of malignant lymphoma, put into 10% neutral formalin solution and fix, in case RNA degradation, fixation time is 24 hours, paraffin embedding (FFPE), so The above-mentioned slides need to be detached with positive charges, and the slices are baked in a 40°C oven for more than 3 hours;

[0162] (2) Carry out dewaxing treatment according to the sample processing method of RNASCope to block the endogenous peroxidase activity in the sample, enhance permeability and expose RNA molecules;

[0163] (3) Design probes: design specific primers according to the imprinted gene sequence;

[0164] The design probe is based on imprinted genes Z1 (Gnas), Z4 (Igf2r), Z5 (Mest), Z6 (Plagl1), Z8 (Dcn), Z11 (Grb10), Z13 (Sgce) and Z16 (Snrpn / Snurf) To design, specifically select a sequence in t...

Embodiment 2

[0174] Example 2 Imprinted gene analysis of lymph node biopsy samples from patients with lymphoma

[0175] The lymph node biopsy sample is to take out suspicious lymph node tissue by puncture, fix with 10% neutral formalin solution for more than 24 hours, and other detection methods are the same as in Example 1.

[0176] From Figure 3(a)-Figure 3(f) It can be seen that each gene of Z1, Z4, Z5, Z6, Z8, Z11, Z13, and Z16 is different in its response sensitivity to malignant lymphoma or in other words, the strength and status of the imprint deletion corresponding to the expression of malignant lymphoma are different.

[0177] Specifically, the sensitivity of each imprinted gene to malignant lymphoma is as follows: Figure 4(a)-Figure 4(h) ,From Figure 4(a)-Figure 4(c) It can be seen that the imprinted gene Z1's imprinted deletion, abnormal copy number and increased expression increase rapidly in the malignant potential stage, slow down in the early and middle malignant lympho...

Embodiment 377

[0179] Example 3 Imprinted Gene Analysis of 77 Lymphoma Samples

[0180] Tissues including lymph node biopsy samples were obtained from 77 patients with malignant lymphoma, and the detection method was the same as in Example 1.

[0181] From Figure 5(a)-Figure 5(h) It can be seen that the proportions of imprinted deletions and copy number abnormalities of 8 probes in 77 lymphoma tissue samples showed a distribution from low to high. According to the distribution trends of different probes, we calculated the dotted line in the figure. Grading standard, the imprint deletion, copy number abnormality and total expression of each probe can be divided into 5 grades from low to high.

[0182] The specific classification is as follows:

[0183] It can be seen from Figure 5(a) that for the imprinted gene Z1, any one of the missing expression of the imprinted gene is less than 16%, the abnormal expression of the copy number of the imprinted gene is less than 1.5%, or the total expres...

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Abstract

The invention relates to a grading model for detecting lymphoma and lymphatic metastatic cancer and application of the grading model. According to the model, change of imprinting genes in lymphoma andlymphatic metastatic cancer is graded by calculating imprinting gene deletion expression quantity, imprinting gene copy number abnormal expression quantity and imprinting gene total expression quantity. The expression of imprint deletion on tissues and cell samples of lymphoma and cancer lymphatic metastasis patients is represented by an intuitive method; through an imprinting gene in-situ labeling method, the change of imprinting genes can be objectively, visually, early and accurately detected, the quantitative model can be provided, and great contribution is made to diagnosis of lymphoma and lymphatic metastatic cancer.

Description

technical field [0001] The present invention relates to the field of biotechnology, to the field of gene diagnosis, to a grading model and its application, to a grading model for detecting the benign and malignant degree of lymphoma and lymphatic metastatic cancer and its application, in particular to a group of imprinted genes in A grading model for detecting the degree of benignity and malignancy of lymphoma and lymphatic metastases and a device composed of the same. Background technique [0002] Lymphoma is a malignant tumor originating from the lymphatic hematopoietic system, mainly caused by viral infection, immunodeficiency, ionizing radiation and chemical drugs, etc., and usually occurs in young adults aged 20-40. In 2012, there were 386,000 new cases and 200,000 deaths of malignant lymphoma in the world, and about 88,000 newly diagnosed cases and 52,000 deaths in China, and it is still growing at an annual rate of 4% in recent years. Lymphoma can occur in any part o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B5/00G16B25/10G16B20/20G16B40/00
Inventor 成彤周宁
Owner LISEN IMPRINTING DIAGNOSTICS (WUXI) CO LTD
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