Cisplatin-containing medicine, preparation method of cisplatin-containing medicine, medicine composition and application of cisplatin-containing medicine
A drug, cisplatin technology, applied in the field of medicine, can solve the problems of impact, reliability of small molecule drug cisplatin delivery, limited clinical application of cisplatin, toxicity and self-design contradiction of transfection activity, etc.
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[0156] According to the second aspect of the present application, there is also provided a method for preparing the above-mentioned cisplatin-containing drug, which includes the following steps: providing any one of the above-mentioned nucleic acid nanoparticles; Platinum is mounted on nucleic acid nanoparticles to obtain cisplatin-containing drugs.
[0157] When physically linked, cisplatin typically intercalates physically between GC base pairs. When the covalent link is used for linking, cisplatin usually reacts with the amino group outside the G ring to form a covalent link. The cisplatin-containing drug prepared by the above method can have better targeting after the target head is modified, can stably deliver cisplatin, and has high reliability.
[0158] In a preferred embodiment, the step of mounting cisplatin through physical connection includes: mixing and stirring cisplatin, nucleic acid nanoparticles and the first solvent to obtain a premixed system; precipitating ...
Embodiment 1
[0181] 1. RNA and DNA nanoparticle carriers:
[0182] (1) The base sequences of the three polynucleotides that make up the RNA nanoparticles are shown in Table 1:
[0183] Table 1:
[0184]
[0185]
[0186] (2) Three polynucleotide base sequences of DNA nanoparticles
[0187] The DNA uses the same sequence as the above RNA, except that T is substituted for U. Among them, the molecular weight of chain a is 8802.66, the molecular weight of chain b is 8280.33, and the molecular weight of chain c is 9605.2.
[0188] The a, b, and c strands of the above-mentioned RNA nanoparticles and DNA nanoparticles were all synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.
[0189] 2. Self-assembly experimental steps:
[0190] (1) RNA or DNA single strands a, b, and c are simultaneously mixed and dissolved in DEPC water or TMS buffer at a molar ratio of 1:1:1;
[0191] (2) Heat the mixed solution to 80°C / 95°C (the RNA assembly temperature is 80°C, and the DNA assembly temper...
Embodiment 2
[0202] 1. Seven groups of short-sequence RNA nanoparticle carriers:
[0203] (1) See Table 2 to Table 8 for the base sequences of the three polynucleotides that make up the RNA nanoparticles in seven groups:
[0204] Table 2: R-1
[0205]
[0206] Table 3: R-2
[0207]
[0208] Table 4: R-3
[0209]
[0210] Table 5: R-4
[0211]
[0212] Table 6: R-5
[0213]
[0214] Table 7: R-6
[0215]
[0216] Table 8: R-7
[0217]
[0218] The single strands of the above seven groups of short-sequence RNA nanoparticle carriers were all synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.
[0219] 2. Self-assembly experimental steps:
[0220] (1) RNA single strands a, b, and c are simultaneously mixed and dissolved in DEPC water or TMS buffer at a molar ratio of 1:1:1;
[0221] (2) Heat the mixed solution to 80°C, keep it for 5min and then cool down slowly to room temperature at a rate of 2°C / min;
[0222] (3) Load the product onto an 8% (m / v) non-denat...
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