Primer probe composition, kit and detection method for detecting methylation of human ADD3 and CDH23 genes

A CDH23, primer-probe technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial assay/inspection, etc., can solve the problem of increasing the cost of methylated DNA detection and detection, and is not suitable for rapid screening and diagnosis of prostate cancer and other problems, to achieve the effect of short experimental period, low cost of genetic testing, and high accuracy

Active Publication Date: 2020-05-15
WUXI SHENRUI BIO PHARMA
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Problems solved by technology

This existing technology relies on the exchange chromatographic column to achieve peak separation of methylated DNA detection signal, which is tantamount to increasing the detection cost of meth

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  • Primer probe composition, kit and detection method for detecting methylation of human ADD3 and CDH23 genes
  • Primer probe composition, kit and detection method for detecting methylation of human ADD3 and CDH23 genes
  • Primer probe composition, kit and detection method for detecting methylation of human ADD3 and CDH23 genes

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Embodiment Construction

[0044]The present invention will be described in detail below in conjunction with the implementations shown in the drawings, but it should be noted that these implementations are not limitations of the present invention, and those of ordinary skill in the art based on the functions, methods, or structural changes made by these implementations Equivalent transformations or substitutions all fall within the protection scope of the present invention.

[0045] Briefly speaking, a primer probe composition, kit and detection method for detecting human ADD3 and CDH23 gene methylation disclosed in this application can detect DNA methylation of human ADD3 and CDH23 gene methylation Screening markers for prostate cancer, avoiding biopsy and relying on PSA indicators for prostate cancer screening, and various problems and drawbacks, and can be used for early auxiliary screening of prostate cancer, so as to provide targeted drug therapy or Surgical treatment provides an accurate basis. M...

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Abstract

The invention provides a primer probe composition, a kit and a detection method for detecting methylation of human ADD3 and CDH23 genes. The primer probe composition comprises a first specific primerand a first specific probe for detecting methylation of the human ADD3 gene, a second specific primer and a second specific probe for detecting methylation of the human CDH23 gene, and a third specific primer and a third specific probe for detecting a reference gene beta-actin. According to the primer probe composition, the kit and the detection method disclosed by the invention, specific detection of the methylation of the human ADD3 and CDH23 genes can be carried out simultaneously, accuracy of early screening and diagnosis of human prostate cancers can be realized, and the primer probe composition, the kit and the detection method are technologically advantaged in high sensitivity, high specificity, low cost, high throughput, simple and convenient operation, short experimental period and lower detection cost of genes.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a primer probe composition, a kit and a detection method for detecting the methylation of human ADD3 and CDH23 genes. Background technique [0002] Prostate cancer is a malignant tumor with high incidence in men. In the United States, the incidence of prostate cancer ranks first among male malignant tumors, and its mortality rate is second only to lung cancer. With changes in life, eating habits, and the environment, the incidence of prostate cancer in my country has increased rapidly in recent years. In developed cities such as Shanghai and Beijing, prostate cancer has become the number one malignant tumor of the male urinary system. [0003] At present, the early detection methods of prostate cancer are mainly the detection of the concentration of prostate cancer antigen PSA in serum and digital rectal examination. Patients with abnormal tests require further prostate ...

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 盛青松朱桂春姚鲁帅于垚垚
Owner WUXI SHENRUI BIO PHARMA
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