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Detection method for embryo chromosomes of embryo culture solution at blastomere stage

A technology of embryo culture medium and detection method, which is applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as no practical application, and achieve the effect of avoiding the problem of sample loss, easy to carry out, and simple to operate.

Pending Publication Date: 2020-05-19
FIRST PEOPLES HOSPITAL OF YUNNAN PROVINCE +1
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Problems solved by technology

[0004] At present, the detection of embryo culture medium is concentrated on the D5 blastocyst stage (fifth day after fertilization), when the number of cells is large, and the DNA content in the culture medium is also high; but for the D3 blastomere stage (the third day after fertilization) ) has not yet been used in practice

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  • Detection method for embryo chromosomes of embryo culture solution at blastomere stage
  • Detection method for embryo chromosomes of embryo culture solution at blastomere stage
  • Detection method for embryo chromosomes of embryo culture solution at blastomere stage

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with accompanying drawing:

[0024] Such as figure 1 and 2 Shown, the present invention comprises the following steps

[0025] A Obtaining of embryo culture medium: ICSI method is used to obtain fertilized eggs by single sperm injection, culture them individually in a culture medium with a volume of no more than 30ul, and transfer them to newly prepared sacs after cultured to the blastomere stage on the third day Blastocyst culture in embryo culture microdrops;

[0026] Collection of B embryo culture fluid: the original embryo culture fluid is collected and transferred to the lysate for centrifugation and mixing;

[0027] C Whole-genome amplification of trace DNA in embryo culture medium: add lyase to the mixture of embryo culture medium and lysate, mix well and incubate, then inactivate the lyase, take out the cleavage product and add it to the PCR reaction tube Genome amplification reaction;

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Abstract

The invention discloses a detection method for embryo chromosomes of an embryo culture solution at the blastomere stage. The embryo culture solution is collected by fertilizing eggs in an ICSI mannerwithout any damage to embryos, the problem of possible sample biopsy trauma in the PGS (preimplantation genetic screening) process is effectively solved, operation is simple, convenient and easy to carry out; and the detection method is suitable for people with high incidence of embryo chromosome abnormality, such as high age, repeated abortion, repeated implantation failure, severe oligozoospermia, chromosome abnormality and the like. According to the detection method, chromosome detection can be carried out at the early stage of embryo culture and prior to the blastula stage, and detection time is brought forward by two days.

Description

technical field [0001] The invention relates to a detection method, in particular to an embryo chromosome detection method in blastomere stage of embryo culture fluid. Background technique [0002] Preimplantation Genetic Diagnosis (PGD) and Preimplantation Genetic Screening (PGS) are genetic testing for embryos cultured in vitro before implantation into the mother. The detection samples of PGD and PGS mainly come from the cells of embryo biopsy. According to the different development stages of in vitro embryos, a single (embryo cleavage stage) or a few cells (blastocyst stage) are selected, and then tested by fluorescence in situ hybridization (Fluorescence in situ hybridization). in Situ Hybridization (FISH) technology or high-throughput sequencing technology (next generation sequencing, NGS) to analyze the genetic material carried by the biopsy tissue, confirm the status of its chromosomes and gene sequences, and select embryos with normal chromosomes for transplantation ...

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Application Information

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IPC IPC(8): C12Q1/6869
CPCC12Q1/6869C12Q2531/113C12Q2535/122C12Q2521/107C12Q2521/101
Inventor 晏家骢李永刚武晓茸李明颖相立峰曾鹏李云秀白云
Owner FIRST PEOPLES HOSPITAL OF YUNNAN PROVINCE
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