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A kind of chitosan drug loading system and its preparation method and application

A drug-carrying system, chitosan technology, applied in the field of preparation of tumor treatment materials, can solve the problems of chemotherapy failure, drug tolerance, etc., and achieve a strong inhibitory effect

Active Publication Date: 2022-03-22
SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Pancreatic cancer is a common malignant gastrointestinal tumor. The current standard drug for clinical treatment of pancreatic cancer is gemcitabine, although gemcitabine and gemcitabine-based combination therapy have played a certain role in prolonging the survival of patients and improving their survival. , but most patients develop drug resistance after receiving treatment for several weeks, leading to chemotherapy failure

Method used

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  • A kind of chitosan drug loading system and its preparation method and application
  • A kind of chitosan drug loading system and its preparation method and application
  • A kind of chitosan drug loading system and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 Modified Chitosan Loading CPI-613 and Gemcitabine

[0040] 1. PLLA loaded with CPI-613

[0041] 1) Dissolve 100mg of PLLA in 1mL of dichloromethane, place in a shaker, and shake at 150rpm for 30min; then add 10mg of CPI-613 to form a primary emulsifier, and shake for 2min. Then add 3 mL of 3% polyvinyl alcohol aqueous solution to form a secondary emulsifier, and shake for 2 minutes. Continue to add 20mL of 0.3% PVA aqueous solution, place it in an ultrasonic machine, and after ultrasonication at 450W for 10min, dilute the solution to 50mL, centrifuge to remove the supernatant, and collect microspheres.

[0042] 2. PLLA-CPI613 was further loaded into modified chitosan

[0043]1) Weigh 100mg modified chitosan

[0044] 2) Add 10 mL of 0.5% acetic acid, place in a shaker, 150 rpm, and shake overnight to obtain a modified chitosan acetic acid solution.

[0045] 3) Take 10mg of PLLA particles containing CPI-613, add it to 10mL modified chitosan acetic acid so...

Embodiment 2

[0047] Example 2 Effect of CPI-613 on the proliferation of pancreatic cancer cells

[0048] 1. Experimental materials

[0049] (1) Drug: CPI-613 in this example is the experimental drug.

[0050] The chemical structural formula of CPI-613 is as follows:

[0051]

[0052] (2) Pancreatic cancer cells: human pancreatic cancer cells BxPC-3 and PANC-1.

[0053] (3) CellTiter-Glo, a commercially available cell proliferation detection kit.

[0054] 2. Experimental grouping

[0055] (1) Control group: blank control, that is, the cancer cells were not treated with any drugs.

[0056] (2) Experimental group: Pancreatic cancer cells were treated with CPI-613 at different concentrations and at different times.

[0057] 3. CellTiter-Glo kit detects the effect of CPI-613 on the proliferation of pancreatic cancer cells

[0058] (1) Spread 8,000 pancreatic cancer cells in a 96-well plate, add 0 μM, 50 μM, 70 μM, and 90 μM CPI-613 to treat the cells for 24 h, 48 h, and 72 h, and then ...

Embodiment 3

[0073] Example 3 Effect of CPI-613 combined with gemcitabine on the proliferation of pancreatic cancer cells

[0074] 1. Experimental materials

[0075] (1) medicine: with embodiment 1

[0076] (2) cancer cell: with embodiment 1

[0077] (3) CellTiter-Glo, a commercially available cell proliferation detection kit.

[0078] 2. Experimental grouping

[0079] (1) Control group: blank control, that is, pancreatic cancer without any drug treatment.

[0080] (2) Single medication group 1: use gemcitabine to treat pancreatic cancer cells.

[0081] (3) Single medication group 2: Pancreatic cancer cells were treated with CPI-613.

[0082] (4) Combined drug group: use CPI-613 and gemcitabine to treat pancreatic cancer cells.

[0083] 3. The effect of CPI-613 combined with gemcitabine on the proliferation of BxPC-3 and PANC-1 cells was detected by CellTiter-Glo method.

[0084] (1) Pancreatic cancer cells were spread in a 96-well plate, and after they adhered to the wall, the cont...

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Abstract

The invention belongs to the technical field of preparation of tumor treatment materials, and specifically discloses a chitosan drug-carrying system, a preparation method and an application thereof. The chitosan drug-carrying system specifically includes a chitosan drug carrier and loaded gemcitabine and CPI-613. In the present invention, CPI-613 combined with gemcitabine significantly inhibits the proliferation of pancreatic cancer cells and the release of mitochondrial ATP, has a significant curative effect on pancreatic cancer, and can be applied to the prevention and treatment of pancreatic cancer. At the same time, gemcitabine combined with CPI-613 chitosan contains The drug system has a stronger inhibitory effect on the proliferation of pancreatic cancer cells than the free state. The invention provides a new drug dosage form and treatment approach for the treatment of pancreatic cancer.

Description

technical field [0001] The invention belongs to the technical field of preparation of tumor treatment materials, in particular to a chitosan drug-carrying system and its preparation method and application. Background technique [0002] Pancreatic cancer is a common malignant gastrointestinal tumor. The current standard drug for clinical treatment of pancreatic cancer is gemcitabine, although gemcitabine and gemcitabine-based combination therapy have played a certain role in prolonging the survival of patients and improving their survival. , but most patients develop drug resistance after receiving treatment for several weeks, leading to chemotherapy failure. On the one hand, 90% of pancreatic cancer tissue is surrounded by a large amount of connective tissue, the cancer tissue area is highly fibrotic, and the blood supply of cancer cells is insufficient, resulting in a low concentration of drugs reaching the local area of ​​pancreatic cancer tissue. Types and growth factors...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/52A61K47/36A61K31/7068A61K31/20A61P35/00
CPCA61K9/5036A61K31/7068A61K31/20A61P35/00A61K2300/00
Inventor 黄林旋石松庆凌世长郭东波张洋何佳鹏游柏浩缑泽明龚军松
Owner SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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