A method for continuous batch induction of Atlantic salmon triploid

Abstract

The invention relates to chromosome manipulation technology, in particular to a method for continuous batch induction of Atlantic salmon triploid. Atlantic salmon sperm eggs are collected for artificial insemination, and the fertilized eggs are divided into 2-3 groups, and each group is successively subjected to hydrostatic pressure induction treatment to inhibit the discharge of its second polar body, thereby obtaining Atlantic salmon triploid; Pressure induction is, after fertilization 300-400 degrees (temperature ℃ × time min), the fertilized eggs of the first group are subjected to hydrostatic pressure treatment to obtain triploid Atlantic salmon; after the fertilized eggs of the first group are taken out, immediately The second group of fertilized eggs was induced by hydrostatic pressure according to the above process, so that the same batch of fertilized eggs could be continuously induced and treated. By adopting the method of the invention, 100% Atlantic salmon triploids can be continuously and efficiently induced.

Description

technical field [0001] The invention relates to chromosome manipulation technology, in particular to a method for continuous batch induction of Atlantic salmon triploid. Background technique [0002] Atlantic salmon (Salmo salar), belonging to the order Salmoniformes and the family Salmonidae, commonly known as "salmon", is of high quality and belongs to high-end edible fish. With the development of my country's land-based industrial circulating water, deep-water cages, and farming workboats in recent years, as well as the continuously increasing market demand, the Atlantic salmon farming industry has gradually emerged. Under natural conditions, Atlantic salmon is generally sexually mature at the third age. During the process of sexual maturity, most of the energy is used for gonad development, resulting in slow growth and sharp decline in meat quality, thereby affecting its nutritional and economic value. In the process of artificial breeding, the phenomenon of precocious ...

AI Technical Summary

Problems solved by technology

Artificial induction of Atlantic salmon triploid has not been seen in China

It has been reported abroad in the 1980s that the pressure shock method can stably obtain triploids with a high induction rate, but its induction parameters are limited to several levels of treatment pressure and treatment time, and the survival rate is also low. Therefore, the optimal induction parameters or parameter ranges of Atlantic salmon triploid still need to be perfected

At the same time, the pressure shock method is limited by the volume of the pressure chamber, and the amount of fertilized eggs processed at one time is limited. Increasing the volume of the pressure chamber or increasing the number of presses will greatly increase the cost and difficulty of operation, which is unrealistic

If large-scale production is to be carried out, it can only be carried out by batch insemination and batch induction. The operation is cumbersome and the quality of fertilized eggs cannot be guaranteed, so the stability of the induction effect is poor

Often the same batch of fertilized eggs cannot be processed, and more triploids cannot be induced, resulting in a lot of waste and loss

So far, there have been no relevant technical reports on the same batch of fish eggs grouped and processed multiple times in a row at home and abroad

In addition, the hatching time of Atlantic salmon is long, and it takes more than 60 days to hatch. Most of the existing reports are for ploidy identification of hatched fry, which takes a long time to evaluate the effect of triploid induction, which brings inconvenience to its subsequent production management.

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