Trichoderma harzianum JSNL1404 and application thereof

A technology of microbial strains and bacterial agents, applied in applications, chemicals for biological control, biocides, etc., can solve problems such as root rot, economic loss, leaves, young shoots withering and dying, and achieve easy preservation and anti-corrosion. The effect of strong reverse ability and simple cultivation conditions

Active Publication Date: 2015-10-21
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, for more than ten years, with the continuous expansion of the artificial cultivation area of ​​Dendrobium candidum, the occurrence of diseases is common and serious. Under warm and humid environmental conditions, the occurrence of Sclerotium rolfssii is more common, which can cause root rot. Leaves and young shoots wither and die, which is a devastating disease that causes huge economic losses. For this reason, it is of practical significance to carry out the prevention and treatment of Dendrobium officinale

Method used

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  • Trichoderma harzianum JSNL1404 and application thereof
  • Trichoderma harzianum JSNL1404 and application thereof
  • Trichoderma harzianum JSNL1404 and application thereof

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Effect test

Embodiment 1

[0040] The characteristics of embodiment 1 Trichoderma harzianum (Trichoderma harzianum) JSNL1404 bacterial strain

[0041] The morphology and physiological activity characteristics of Trichoderma harzianum (Trichoderma harzianum) JSNL1404 described in the present invention are as follows:

[0042] Trichoderma harzianum (Trichoderma harzianum) JSNL1404 grows fast on the PDA medium plate of the PRX-250A type intelligent artificial climate box at 25 ± 1 ℃ (RH70%, dark), and the average daily growth is 2.7cm. White, fading to light green. The optimum temperature is 30°C, and it grows better at 35°C. Conidia are subspherical to ovoid, smooth surface, light green, 2.8-3.2×2.5-2.8μm in size.

[0043] The mycelia of Trichoderma harzianum JSNL1404 grew best at 25±1°C and pH 4-6, with an average daily growth of 2.6 cm; at 30°C, the sporulation was the highest at pH 6-8. The optimum growth temperature of the bacteria is 25±1℃.

[0044] The rDNA-ITS sequence of Trichoderma harzianum ...

Embodiment 2

[0046] The preparation method of Trichoderma harzianum (Trichoderma harzianum) JSNL1404 inoculum: Inoculate Trichoderma harzianum JSNL1404 in liquid fermentation medium, pH 4.5, liquid volume in 500mL Erlenmeyer flask is 250mL, 25°C-30°C, shaker speed 120 revs / min, 72 hours, the fermented liquid that obtains is the original bacterium liquid of preventing and treating Dendrobium candidum candidum.

[0047] Wherein the formula of liquid fermentation medium is: 1000mL distilled water, 120g potato, 15g sucrose, 0.5g ammonium sulfate, pH4.5.

Embodiment 3

[0049] The preparation method of Trichoderma harzianum (Trichoderma harzianum) JSNL1404 inoculum: inoculate Trichoderma harzianum JSNL1404 in liquid fermentation medium, pH 5.5, liquid volume in 500mL Erlenmeyer flask is 250mL, 25°C-30°C, shaker speed 160 revs / min, 120 hours, the fermented liquid that obtains is the original bacterium liquid of preventing and treating Dendrobium candidum candidum.

[0050] Wherein the formula of liquid fermentation medium is: 1000mL distilled water, 150g potato, 18g sucrose, 1.0g ammonium sulfate, pH5.5.

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Abstract

The invention discloses trichoderma harzianum JSNL1404. The type of the trichoderma harzianum JSNL1404 is named as trichoderma harzianum. The trichoderma harzianum JSNL1404 is preserved in the general microbiological center of China General Microbiological Culture Collection Center on June 9th, 2014, and the preservation number is CGMCC No.9281. The invention further discloses the application of the trichoderma harzianum JSNL1404. The trichoderma harzianum JSNL1404 is mainly used for preventing and controlling dendrobium officinale southern blight germs, has the restraining function on multiple kinds of phytopathogen and plays an important role in antagonistic fungi plant disease biological control. The trichoderma harzianum JSNL1404 has the advantages that the number of types is large, the amount of the trichoderma harzianum is large, multiple action modes on pathogenic bacteriae are provided, the breeding speed is high, artificial culturing can be achieved, operation is easy, production and application are convenient, and the adversity resistance is high. Especially, the trichoderma harzianum JSNL1404 has a good preventing and controlling effect on southern blight caused by fungus.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a Trichoderma harzianum JSNL1404 and application thereof. Background technique [0002] Trichoderma is a fungus that widely exists in nature, and Trichoderma harzianum is the most widely used species of Trichoderma. Trichoderma (Trichoderma spp.) belongs to the subphylum Deuteromycetes, the class Hyphomycetes, the order Polychoderma, and the family Polypodiaceae. Widely present in soil, root circle, leaf circle, seeds and other ecological environments, it is also an excellent biocontrol bacteria. In 1932, Weindling discovered that Trichoderma lignorum can parasitize a variety of plant pathogenic fungi, and suggested that the fungus be used for the biological control of soil-borne plant diseases, and the research on the biocontrol of Trichoderma began from then on. Due to its wide adaptability, broad spectrum and multi-mechanism, Trichoderma has always been the focus of research ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 赵桂华谢春芹赵楠杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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