Bionic alveolus culture method and bionic alveolus culture device

Abstract

The invention provides a bionic alveolus culture method and a bionic alveolus culture device. The bionic alveolus culture device comprises an incubator, a cell growth membrane and a first plugging piece; the incubator is provided with an accommodating chamber; the cell growth membrane is arranged in the accommodating chamber, an accommodating space for accommodating a cell culture solution is formed between the cell growth membrane and the wall of the accommodating chamber, the cell growth membrane comprises a sphere and a pipe body communicating with the sphere, the sphere accommodates cell suspension, and the pipe body is in fixed seal joint with the incubator; the first plugging piece is used for plugging a pipe orifice of the pipe body; a standing structure facilitating standing is arranged at the bottom of the incubator, the incubator is also provided with at least three flat side faces facilitating flat placement, and the standing structure is combined with the flat side faces toensure that the incubator is in a vertical posture and a horizontal posture with multiple angles in the circumferential direction, so that the inner wall of the sphere are evenly inoculated with cells. The bionic alveolus culture method and the bionic alveolus culture device have the advantages that even if the influence of gravity factor exists, the cells can intensively grow at different positions; and compared with the prior art, the cells are more uniform, a more real three-dimensional structure of alveolar vesicular cells can be simulated, and the accuracy of subsequent experimental results is improved.

Description

technical field [0001] The invention relates to a bionic alveolar culture method and a culture device. Background technique [0002] As an intervention to rescue dyspnea caused by lung injury diseases, mechanical ventilation can be divided into invasive mechanical ventilation and non-invasive mechanical ventilation in clinical practice. Invasive mechanical ventilation can also cause adverse effects in the treatment of some diseases. It was found that invasive mechanical ventilation is an independent risk factor for the death of COPD patients admitted to the acute respiratory failure intensive care unit (ICU). Studies have shown that the main reason for this result is that the tidal volume generated by traumatic mechanical ventilation acts on the alveoli and causes lung injury by forming a certain mechanical force. [0003] Alveoli are sacs composed of a single layer of epithelial cells that surround the enlarged sacs at the ends of the pulmonary bronchioles. As the basic v...

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Problems solved by technology

However, in practical applications, due to the existence of gravity, the cells usually grow concentratedly at the bottom of the cell growth membrane, and the cell growth is uneven, which cannot simulate the most realistic alveolar vesicle-like three-dimensional cell structure, which will affect the follow-up experimental results. accuracy

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