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Markers for detecting malignant tumors, tumor metastasis and tumor types and application of markers

A technology of malignant tumors and markers, which is applied in the field of detection of malignant tumors, markers of tumor metastasis and types, and detection of tumor markers. It can solve the problems of not clearly distinguishing diagnostic markers and achieve high correlation effects

Pending Publication Date: 2020-05-29
LISEN IMPRINTING DIAGNOSTICS WUXI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above reasons, there are currently no diagnostic markers for tumor metastasis and tumor types that can be clearly distinguished. Analyzing the changes in molecular markers of tumor metastasis and tumor types at the cellular level can provide more accurate prediagnosis and diagnostic information. , and guide the selection of treatment options

Method used

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  • Markers for detecting malignant tumors, tumor metastasis and tumor types and application of markers
  • Markers for detecting malignant tumors, tumor metastasis and tumor types and application of markers
  • Markers for detecting malignant tumors, tumor metastasis and tumor types and application of markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 118

[0082] Example 1 Imprinted gene detection of 18 cases of metastatic and non-metastatic thyroid cancer samples

[0083] 18 thyroid cancer samples, including metastatic and non-metastatic samples, were obtained by puncture, and imprinted gene detection was performed as follows:

[0084] (1) Thyroid cancer samples were obtained by puncture, fixed in 10% neutral formalin solution to prevent RNA degradation, the fixation time was 24 hours, and adhered to a glass slide, which needs to be positively charged Anti-shedding glass slides, bake in a 37°C oven for more than 3 hours;

[0085] (2) Follow the sample processing method of RNASCope to block the endogenous peroxidase activity in the sample, enhance permeability and expose RNA molecules;

[0086] (3) Design probes: design specific primers according to the imprinted gene sequence;

[0087] The design probe is designed according to the imprinted gene Grb10, specifically, a sequence is selected in the inner rotor of the gene as the...

Embodiment 216

[0097] Example 2 Imprinted gene detection of sentinel lymph node aspiration samples from 16 cases of metastatic and non-metastatic breast cancer

[0098] The sentinel lymph node samples of 16 cases of breast cancer were obtained by puncture, including metastatic and non-metastatic samples, and other detection methods were the same as in Example 1.

[0099] It can be seen from Figure 2(a) that the imprinted gene Grb10 imprinted deletion expression in sentinel lymph node samples of non-metastatic breast cancer is mostly lower than 5%, and the imprinted gene Grb10 imprinted deletion expression in sentinel lymph node samples of metastatic breast cancer is mostly higher than 5%; as can be seen from Figure 2(b), the abnormal expression of the copy number of the imprinted gene Grb10 in the sentinel lymph node samples of non-metastatic breast cancer is lower than 0.5%, and the abnormal expression of the copy number of the imprinted gene Grb10 in the sentinel lymph node samples of metas...

Embodiment 3

[0100] Example 3 Imprinted gene detection of small cell lung cancer cell lines and non-small cell lung cancer samples

[0101] Obtain three cultured small cell lung cancer cell lines, H446, H69 and DMS114, obtain non-small cell lung cancer samples by puncture, design probes based on imprinted genes Peg3 and Slc38a4, and other detection methods are the same as

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Abstract

The invention relates to markers for detecting malignant tumors, tumor metastasis and tumor types and application of the markers. The tumor markers comprise thyroid tumor markers, breast cancer sentinel lymph node metastasis markers, tumor markers for distinguishing lung cancer types, malignant tumor markers, early gastric cancer diagnosis markers and non-solid tumor markers. By adopting the markers provided by the invention, metastasis of thyroid cancer and breast cancer can be accurately detected, small cell lung cancer and non-small cell lung cancer can be distinguished, early gastric cancer can be accurately detected, non-entity tumors can be detected, an instruction function on operative therapy method and medicine selection for thyroid cancer, breast cancer, lung cancer, gastric cancer and non-entity tumors can be achieved, and the life of a patient can be prolonged.

Description

technical field [0001] The invention relates to the field of biotechnology, to the field of gene diagnosis, to a marker for detecting tumors, in particular to a marker for detecting malignant tumors, tumor metastasis and types and applications thereof. Background technique [0002] Cancer is a major threat to human life. There are 14 million new cancer patients worldwide every year, and 8.2 million patients die of cancer at the same time. Unlimited proliferation and metastasis are important characteristics of cancer cells. Most cancer patients die due to extensive metastasis of cancer cells, causing complications such as organ failure. Many cancers will begin to metastasize at an early stage. At this time, the number of cancer cells metastasized from the cancer focus is still small, and stable metastases have not yet formed. If effective chemotherapy drugs can be used in time, the metastatic cancer cells will be eliminated. It is of great significance to prevent postoperati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/136
Inventor 成彤周宁
Owner LISEN IMPRINTING DIAGNOSTICS WUXI CO LTD