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Visual background device, fluorescence microscopic optical system and scanning analysis system

A visual background and optical system technology, applied in the direction of material analysis, fluorescence/phosphorescence, measuring devices, etc. through optical means, to achieve the effect of small reflection phenomenon and improve quality

Pending Publication Date: 2020-06-05
BEIJING LINJIN SPACE AIRCRAFT SYST ENG INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the embodiment of the present application, a visual background device of a fluorescent microscopic optical system, a fluorescent microscopic optical system, and a fluorescent dyed cell scanning and analysis system are provided to solve the problem that the visual background device of a fluorescent microscopic optical system reflects excitation light technical problem

Method used

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  • Visual background device, fluorescence microscopic optical system and scanning analysis system
  • Visual background device, fluorescence microscopic optical system and scanning analysis system
  • Visual background device, fluorescence microscopic optical system and scanning analysis system

Examples

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Embodiment 1

[0030] figure 2 It is a schematic diagram of a visual background device of a fluorescence micro-optical system according to an embodiment of the present application; image 3 for figure 2 A schematic diagram of the visual background setup and the objective lens of the fluorescence microscopy optical system is shown.

[0031] like figure 2 and image 3 As shown, the visual background device of the fluorescence micro-optic system of the embodiment of the present application is used for the fluorescence micro-optic system of the fluorescence staining cell scanning and analysis system. One side of the visual background device can provide fluorescence as the background light of the observed object 332, and the side of the visual background device that can provide fluorescence has a non-reflective area 310, and the non-reflective area 310 transmits the excitation light. over or absorbed;

[0032] Wherein, the non-reflection area 310 is used to face the objective lens of the ...

Embodiment 2

[0064] Figure 4 It is a schematic diagram of the fluorescence micro-optic system of the embodiment of the present application; Figure 5 for Figure 4 Schematic diagram of the PWM dimming setup of the fluorescence micro-optical system shown; Image 6 for Figure 5 Schematic diagram of the pulse voltage output by the PWM controller of the shown PWM dimming device.

[0065] Such as Figure 4 As shown, the fluorescence micro-optical system of the embodiment of the present application is used in the scanning and analysis system of fluorescent stained cells, and includes the visual background device described in the first embodiment.

[0066] implementation, such as Figure 4 As shown, the fluorescence micro-optical system further includes an objective lens 331 , a PWM dimming device 333 , and a filter device 334 . Next, the PWM dimming device will be described.

[0067] Such as Figure 5 with Image 6 As shown, the PWM dimming device includes:

[0068] voltage source 22...

Embodiment 3

[0095] A fluorescent staining cell scanning and analysis system according to an embodiment of the present application includes the visual background device described in Embodiment 1.

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Abstract

The embodiment of the invention provides a visual background device, a fluorescence microscopic optical system and a fluorescence staining cell scanning and analyzing system. One side of the visual background device can provide fluorescent light as background light of an observed object, the side, capable of providing the fluorescent light, of the visual background device is provided with a non-reflection area, and the non-reflection area penetrates through or absorbs exciting light, wherein the non-reflection area is used for facing an objective lens of the fluorescence microscopic optical system so as to reduce reflection of the visual background device to the exciting light, and the exciting light is exciting light penetrating through the objective lens of the fluorescence microscopic optical system. The fluorescence microscopic optical system comprises the visual background device. The fluorescence staining cell scanning and analyzing system comprises the visual background device.The fluorescence staining cell scanning and analyzing system comprises the fluorescence microscopic optical system. The technical problem that the visual background device of the fluorescence microscopic optical system reflects the exciting light is solved.

Description

technical field [0001] The present application relates to the technical field of fluorescence microscopy, in particular, to a visual background device of a fluorescence micro-optic system, a fluorescence micro-optic system, and a fluorescent staining cell scanning and analysis system. Background technique [0002] The traditional fluorescent micro-optical system in the fluorescent staining cell scanning and analysis system uses a passive fluorescent lining as the visual background of the observed object, that is, the passive fluorescent lining is used as the visual background device of the fluorescent micro-optical system. principle such as figure 1 As shown, the excitation light 11 passing through the objective lens 10 excites the observed object 20 to generate fluorescence, and the objective lens 10 realizes the research of various organisms by detecting the fluorescence, wherein the objective lens 10 is an epi-fluorescent microscope objective lens. In order to identify t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/6458G01N21/01G01N2021/0112
Inventor 张志龙潘红九商学谦张鹏宇郑宇马鸣李霄吴晓蕊杨晨许宝芝李勇董元洪
Owner BEIJING LINJIN SPACE AIRCRAFT SYST ENG INST