Method for detecting Sudan red dye in fruit and vegetable juice

A detection method, fruit and vegetable juice technology, applied in the field of food safety, can solve the problems of long time-consuming solid phase extraction column, low extraction efficiency of n-hexane, low recovery rate, etc., achieve low detection limit, easy operation, and short analysis time Effect

Active Publication Date: 2020-06-09
GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low extraction efficiency of n-hexane during processing, the use of a large amount of organic solvents, and the time-consuming SPE cartridges, this method is cumbersome to operate and usually has a low recovery rate

Method used

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  • Method for detecting Sudan red dye in fruit and vegetable juice
  • Method for detecting Sudan red dye in fruit and vegetable juice
  • Method for detecting Sudan red dye in fruit and vegetable juice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of Magnetic Porous Carbon Nanofibers

[0020] (1) Dissolve 2.0g PAN and 0.4g PMMA in DMF, heat the solution to 60°C, stir and react for 12 hours, spin the solution obtained by the reaction by electrospinning to obtain nanofiber materials, and spin the obtained The nanofibers were dried in a vacuum drying oven at 60°C for 24 hours, and the dried nanofiber materials were placed in a tube furnace. The temperature of the tube furnace was set at 280°C and kept for 4 hours. Argon gas was introduced, and the temperature was programmed to 1000°C, obtain porous carbon nanofiber material, grind for later use;

[0021] (2) Get the porous carbon nanofiber material prepared in step (1), ultrasonically disperse it in aqueous solution, keep the nitrogen environment, add FeCl 3 ·6H 2 O and FeCl 2 4H 2 O, and after heating to 70°C, add ammonia solution dropwise to adjust the pH value to 10, keep the temperature at 70°C and stir for 1 hour, collect magnetic porous carbon ...

Embodiment 2

[0024] Application of magnetic porous carbon nanofibers in the detection of Sudan red dyes in fruit and vegetable juices, refer to Figure 2-Figure 3 .

[0025] (1) Take 10 mL of commercially available fruit and vegetable juice in a centrifuge tube, add 3 mg of the magnetic porous carbon nanofiber, add 0.8 g of NaCl, shake and mix for 15 min, use a magnet to adsorb the solid-phase adsorbent, pour the extracted liquid, and use 4 mL of ethanol eluting the adsorbent;

[0026] (2) Evaporating the eluent to dryness under reduced pressure to obtain a residue;

[0027] (3) The residue was redissolved with 200 μL of acetonitrile, filtered through a 0.22 μm organic filter, and then injected into HPLC for determination.

[0028] The fruit and vegetable juice samples without any treatment and the fruit and vegetable juice samples after magnetic solid phase extraction were detected at wavelengths of 478nm, 520nm, and 538nm, and the detection results were as shown, image 3 1 to 5 repre...

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Abstract

The invention relates to the field of food safety, in particular to a method for detecting Sudan red dye in fruit and vegetable juice. A magnetic porous carbon nanofiber material is prepared through the method, and the magnetic porous carbon nanofiber material is large in specific surface area and has a porous structure; the magnetic porous carbon nanofiber material is used for magnetic solid-phase adsorption extraction of the Sudan red dye in the fruit and vegetable juice, and finally the Sudan dye is analyzed and detected by using a high performance liquid chromatography tandem PDA detector.The method has the advantages of simplicity in operation, high extraction efficiency, low organic solvent consumption, high sensitivity and the like.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a method for detecting Sudan red dye in fruit and vegetable juices. Background technique [0002] In recent years, my country's economic level has improved significantly, people's quality of life has also been greatly improved, and the emphasis on food safety has also increased year by year. [0003] Sudan red dye is a synthetic azo dye. Because of its special chemical structure, the metabolites of this compound have obvious toxicity to the skin, liver, kidney and other organs of the body, so it is listed as a third-level carcinogen. Sudan red dye is often used in industrial printing and dyeing, such as shoe polish, floor color enhancement, etc. In recent years, unscrupulous merchants have taken advantage of the bright, long-lasting color and low price of Sudan red dye to add it to foods such as chili powder, seasoning sauce, fermented bean curd and sausage. [0004] Due to the compl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08
CPCG01N30/02G01N30/06G01N30/08
Inventor 黎鹏孟飞聂梅黄俊生刘泽槟汤静洁黄东婷
Owner GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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