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Application of DIO2 in preparation of medicine for predicting or treating intrauterine adhesion

An intrauterine adhesion and drug technology, applied in the field of biomedicine, can solve problems affecting endometrial fibrosis and the like

Active Publication Date: 2020-06-12
NANJING DRUM TOWER HOSPITAL +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] DIO2 is an enzyme that converts thyroxine T4 into active thyroxine T3. In the lungs of patients with idiopathic pulmonary fibrosis, the activity and expression level of DIO2 was significantly higher than that of controls and correlated with the severity of the disease. However, Whether there is a correlation between DIO2 and intrauterine adhesions, and whether it affects endometrial fibrosis has not been reported

Method used

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  • Application of DIO2 in preparation of medicine for predicting or treating intrauterine adhesion
  • Application of DIO2 in preparation of medicine for predicting or treating intrauterine adhesion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2DI

[0035] Example 2 Effect of DIO2 on fibrosis of endometrial epithelial cells

[0036] 1. Materials, reagents, equipment

[0037] 1.1 Main reagents

[0038] Type I collagenase (Sigma), hyaluronidase (Sigma), DNAase (Roche), epithelial cell culture medium (Gibco), DMEM / F12 medium (Wisent Inc), serum (Gibco), Trizol (Invitrogen). TGFβ1 (PeproTech), thyroxine T4 / T3 (MCE), FN / N-cad / E-cad / a-SMA / DIO2 antibody (abcam), electrophoresis buffer, transfer buffer, skimmed milk (bio-rad), TBST

[0039] 1.2 Main instruments

[0040] Cell incubator, shaker, PVDF nitrocellulose membrane, double vertical electrophoresis tank, gel imaging analysis system

[0041] 1.3 Main method

[0042] 1.3.1 Isolation and culture of primary endometrial epithelial cells

[0043] Place the endometrial tissue in a new 60mm petri dish, cut the tissue until no lumps are visible to the naked eye, add the prepared digestive solution, blow and mix, place in a 37°C incubator for 3 minutes; take out the petri dish, ...

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Abstract

The invention discloses application of DIO2 in preparation of a medicine for predicting or treating intrauterine adhesion. It is found that DIO2 expression in endometrium of a patient suffering from intrauterine adhesion is reduced, and particularly, epithelial cell reduction is most remarkable. In-vitro results show that DIO2 can effectively reverse EMT of endometrial epithelial cells by converting thyroxine T4 into T3, and fibrosis of the epithelial cells is inhibited. Therefore, DIO2 may play an important role in the aspects of intrauterine adhesion diagnosis and treatment, and can be usedfor clinical diagnosis of intrauterine adhesion and preparation of drugs for treating intrauterine adhesion and other diseases related to uterine fibrosis.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of DIO2 in the preparation of medicines for predicting or treating intrauterine adhesions. Background technique [0002] Intrauterine adhesion (IUA) is severe damage to the endometrium caused by a variety of factors, resulting in adhesion between the inner walls of the uterus, complete or partial closure of the uterine cavity, and the epithelial and stromal cells that constitute the endometrium are surrounded by fibrotic stroma and Epithelial replacement of proliferation and differentiation disorders, endometrium unresponsive to sex hormone stimulation. IUA is the most common cause of secondary infertility, accounting for as high as 25-30% of infertile women. The incidence of membrane fibrosis was significantly increased. At present, the main treatment method is hysteroscopic adhesion separation operation, which is not effective for severe extensive endometrial injury, a...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61P15/00C12Q1/6883G01N33/573
CPCA61K38/44A61P15/00C12Q1/6883C12Q2600/158G01N33/573G01N2800/36
Inventor 胡娅莉赵光锋周振华李若天戴建武
Owner NANJING DRUM TOWER HOSPITAL
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