Lactobacillus plantarum p-17 and method for preparing fermented apple juice using the same

A technology of Lactobacillus plantarum and apple juice, applied in the direction of microorganism-based methods, biochemical equipment and methods, Lactobacillus, etc., can solve the problem of high acidity of the product, achieve an increase in antioxidant value, simple production methods, and easy-to-obtain raw material sources Effect

Active Publication Date: 2022-08-02
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem in the fermentation process of apple juice is that the acidity of the obtained product is too high or because the apple juice itself contains less directly usable carbon and nitrogen sources, it needs specific microorganisms to ferment
In addition, apple itself contains antioxidant substances, but the technology of using specific lactobacillus to ferment apple juice to improve its antioxidant activity has not been effectively implemented and reported.

Method used

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  • Lactobacillus plantarum p-17 and method for preparing fermented apple juice using the same
  • Lactobacillus plantarum p-17 and method for preparing fermented apple juice using the same
  • Lactobacillus plantarum p-17 and method for preparing fermented apple juice using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Screening excellent lactic acid bacteria

[0037] (1) Isolation of lactic acid bacteria

[0038] The wine samples during the post-fermentation period were selected, and the strains were isolated by the direct plate separation method. Streak purification was performed on strains that met Gram positive, contact enzyme negative and skim milk curd tests.

[0039] (2) Tolerance comparison of isolated strains

[0040] Using the number of viable bacteria as an indicator, and using Lac450 as a control strain for most wineries, the tolerance to pH, osmotic pressure and temperature of the strains isolated from the post-fermentation period of wine was investigated, so as to screen out the strains with stronger comprehensive tolerance. strains.

[0041] After the isolated strains were activated, 10 7 CFU / mL were respectively inoculated into MRS liquid medium with different pH values ​​(4.0, 3.5, 3.0) and different sugar concentrations (20, 40, 60%) and cultured at 30 ...

Embodiment 2

[0082] Embodiment 2 Lactobacillus plantarum P_17 suitable growth condition and growth curve

[0083] (1) Determination of suitable growth temperature

[0084] The changes of viable bacteria of Lactobacillus plantarum P_17 after culturing for 24h under different temperature conditions are shown in Figure 7 . Depend on Figure 7 It can be seen that low temperature has an inhibitory effect on the growth of Lactobacillus plantarum P_17. Between 17 and 37 °C, the density of bacteria increases rapidly with the increase of temperature. The amount of bacteria showed a downward trend. The test showed that 37℃ was the suitable growth temperature of Lactobacillus plantarum P_17.

[0085] (2) Determination of suitable growth pH

[0086] The changes of viable bacteria of Lactobacillus plantarum P_17 after culturing for 24h under different pH values ​​are shown in Figure 8 . Depend on Figure 8 It can be seen that the lower pH value has a significant inhibitory effect on the growth...

Embodiment 3

[0089] Example 3 In vitro simulation of artificial gastrointestinal digestion by Lactobacillus plantarum P_17

[0090] (1) Tolerance study on artificial gastric juice

[0091] Probiotics must travel through the harsh stomach environment to reach the gut in order to be effective. Food stays in the human gastric juice environment for 1 to 3 hours, and the pH value of human gastric juice is about 3.0 on average. Therefore, probiotics must be resistant to gastric juice in order to exert their functions in the human body. After the lactic acid bacteria were exposed to the artificial gastric juice with pH value of 3.0 for 3 hours, the number of viable bacteria and the survival rate were shown in Table 7.

[0092] Table 7 Test results of tolerance of lactic acid bacteria to artificial gastric juice

[0093]

[0094] It can be seen from Table 7 that after Lactobacillus plantarum P_17 acts in artificial gastric juice with pH value of 3.0 for 3h, the number of viable bacteria is (...

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Abstract

The invention discloses a Lactobacillus plantarum P_17 and a method for preparing fermented apple juice by using the bacteria. The method uses apple juice as a raw material, and is prepared by fermenting Lactobacillus plantarum P_17 whose deposit number is CGMCC NO.19251. Lactobacillus plantarum P_17 has outstanding acid resistance, sugar resistance and low temperature resistance. It has strong pepsin resistance and acid resistance, can resist the stress environment of artificial intestinal juice, and has the ability to tolerate bile salts and high osmotic pressure. The fermented apple juice has uniform color, uniform and delicate organization, and has the unique aroma and apple flavor of fermentation. The fermented apple juice prepared by the method has a higher number of viable bacteria than other bacteria, and the γ-aminobutyric acid, total acid and antioxidant value are obviously improved compared with ordinary apple juice. The method has simple production process, abundant and easily available raw materials, and has far-reaching significance for the industrial production of fruit and vegetable juice lactic acid fermented beverages.

Description

technical field [0001] The invention relates to the technical field of fruit juice fermentation, in particular to a Lactobacillus plantarum P_17 and a method for preparing fermented apple juice by using the Lactobacillus plantarum P_17. Background technique [0002] Apple, belonging to the Rosaceae apple genus, is the most widely planted and most productive fruit. Its taste is sweet and sour, and it is rich in nutrients. According to the determination, every hectogram of apples contains 6.5-11.2g fructose, 2.5-3.5g glucose and 1.0-5.2g sucrose. Apple can not only regulate gastrointestinal function, lower cholesterol, lower blood pressure, prevent cancer, lose weight, but also enhance children's memory. Apple not only contains a variety of vitamins, lipids, minerals, carbohydrates and other nutrients necessary for the formation of the brain, but also contains fine fibers that are conducive to children's growth and development and zinc that can enhance children's memory. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L2/38A23L2/52C12R1/25
CPCA23L2/382A23L2/52A23V2002/00C12R2001/25C12N1/205A23V2200/15A23V2200/30A23V2400/169
Inventor 田洪涛李晨张波张娜牛志华赵丽娜
Owner HEBEI AGRICULTURAL UNIV.
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