The invention discloses portable full nutritional yoghurt for spaceflight, a yoghurt sheet, and a preparation method thereof. The yoghurt comprises proteins originated from plants and animals, multiple plant oils, carbohydrates, vitamins, mineral substances, spirulina, wheat dietary fiber, coenzyme Q10, and xylo-oligosaccharide. The portable full nutritional yoghourt is prepared by the following steps: (1) mixing skim milk and a plurality of saccharides, homogenizing, sterilizing, carrying out enzymatic hydrolysis by using lactase, and cooling; (2) carrying out inoculated fermentation to obtain yoghurt; and (3) evenly mixing the yoghurt with other plant oils, proteins, carbohydrates, vitamins, and mineral substances, homogenizing, pre-freezing, freeze-drying, and sieving to obtain the portable full nutritional yoghurt. The portable full nutritional yoghourt is mixed with D-sorbitol, maltitol, and pre-gelatinized starch, the mixture is pressed into tablets, and finally the tablets are coated to obtain yoghurt sheets. Compared with the common low temperature yoghurt, the provided portable full nutritional yoghurt has the advantages of long shelf life, portability, suitability for spaceflight, full nutrients, low lactose content, and multiple flavors, and can promote the health.