Lactobacillus plantarum P _ 17 and method for preparing fermented apple juice by using lactobacillus plantarum P _ 17
A technology of Lactobacillus plantarum and apple juice, applied in the direction of microorganism-based methods, biochemical equipment and methods, Lactobacillus, etc., can solve the problems of high acidity of products, achieve the improvement of antioxidant value, broad development and application prospects, low temperature resistance The effect of outstanding ability
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Embodiment 1
[0036] Embodiment 1 screens good lactic acid bacteria
[0037] (1) Isolation of lactic acid bacteria
[0038] The wine samples in the post-fermentation period were selected, and the strains were isolated by the direct plate separation method. Streak purification was performed on the strains that met the Gram stain positive, contact enzyme test negative and skim milk curd test.
[0039] (2) Comparison of tolerance of isolated strains
[0040] Using the number of viable bacteria as an index, and Lac450, which is used in most wineries, as a control strain, the strains isolated from the post-fermentation period of wine were investigated for their tolerance to pH, osmotic pressure and temperature, so as to screen out strains with strong comprehensive tolerance. strain.
[0041] After the isolated strain was activated, with 10 7 CFU / mL were respectively inoculated in MRS liquid medium with different pH values (4.0, 3.5, 3.0) and different sugar concentrations (20, 40, 60%) and...
Embodiment 2
[0082] Embodiment 2 Lactobacillus plantarum P_17 suitable growth conditions and growth curve
[0083] (1) Determination of suitable growth temperature
[0084] See Figure 7 . Depend on Figure 7 It can be seen that low temperature has an inhibitory effect on the growth of Lactobacillus plantarum P_17. Between 17°C and 37°C, the density of bacteria increases rapidly with the increase of temperature. The amount of bacteria showed a downward trend, and the test showed that 37°C was the suitable growth temperature for Lactobacillus plantarum P_17.
[0085] (2) Determination of suitable pH value for growth
[0086] See Figure 8 . Depend on Figure 8 It can be seen that the lower pH value has a significant inhibitory effect on the growth of Lactobacillus plantarum P_17. Between 3.0 and 6.0, the density of the bacteria increases rapidly with the increase of the pH value, and the bacterial population reaches the maximum value when the pH value is 6.0. , the pH value continue...
Embodiment 3
[0089] Example 3 Lactobacillus plantarum P_17 simulated artificial gastrointestinal digestion in vitro
[0090] (1) Tolerance study on artificial gastric juice
[0091] Probiotics must pass through the harsh environment of the stomach to reach the gut in order to be effective. The food stays in the human gastric juice environment for 1 to 3 hours, and the pH value of the human gastric juice is about 3.0 on average. Therefore, probiotics must have the ability to withstand gastric juice in order to perform their functions in the human body. After the lactic acid bacteria were exposed to the artificial gastric juice with a pH value of 3.0 for 3 hours, the changes in the number of viable bacteria and the survival rate are shown in Table 7.
[0092] Table 7 The tolerance test result of lactic acid bacteria to artificial gastric juice
[0093]
[0094] It can be seen from Table 7 that after the Lactobacillus plantarum P_17 was exposed to artificial gastric juice with a pH valu...
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