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Lactobacillus plantarum P _ 17 and method for preparing fermented apple juice by using lactobacillus plantarum P _ 17

A technology of Lactobacillus plantarum and apple juice, applied in the direction of microorganism-based methods, biochemical equipment and methods, Lactobacillus, etc., can solve the problems of high acidity of products, achieve the improvement of antioxidant value, broad development and application prospects, low temperature resistance The effect of outstanding ability

Active Publication Date: 2020-06-16
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem in the fermentation process of apple juice is that the acidity of the obtained product is too high or because the apple juice itself contains less directly usable carbon and nitrogen sources, it needs specific microorganisms to ferment
In addition, apple itself contains antioxidant substances, but the technology of using specific lactobacillus to ferment apple juice to improve its antioxidant activity has not been effectively implemented and reported.

Method used

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  • Lactobacillus plantarum P _ 17 and method for preparing fermented apple juice by using lactobacillus plantarum P _ 17
  • Lactobacillus plantarum P _ 17 and method for preparing fermented apple juice by using lactobacillus plantarum P _ 17
  • Lactobacillus plantarum P _ 17 and method for preparing fermented apple juice by using lactobacillus plantarum P _ 17

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 screens good lactic acid bacteria

[0037] (1) Isolation of lactic acid bacteria

[0038] The wine samples in the post-fermentation period were selected, and the strains were isolated by the direct plate separation method. Streak purification was performed on the strains that met the Gram stain positive, contact enzyme test negative and skim milk curd test.

[0039] (2) Comparison of tolerance of isolated strains

[0040] Using the number of viable bacteria as an index, and Lac450, which is used in most wineries, as a control strain, the strains isolated from the post-fermentation period of wine were investigated for their tolerance to pH, osmotic pressure and temperature, so as to screen out strains with strong comprehensive tolerance. strain.

[0041] After the isolated strain was activated, with 10 7 CFU / mL were respectively inoculated in MRS liquid medium with different pH values ​​(4.0, 3.5, 3.0) and different sugar concentrations (20, 40, 60%) and...

Embodiment 2

[0082] Embodiment 2 Lactobacillus plantarum P_17 suitable growth conditions and growth curve

[0083] (1) Determination of suitable growth temperature

[0084] See Figure 7 . Depend on Figure 7 It can be seen that low temperature has an inhibitory effect on the growth of Lactobacillus plantarum P_17. Between 17°C and 37°C, the density of bacteria increases rapidly with the increase of temperature. The amount of bacteria showed a downward trend, and the test showed that 37°C was the suitable growth temperature for Lactobacillus plantarum P_17.

[0085] (2) Determination of suitable pH value for growth

[0086] See Figure 8 . Depend on Figure 8 It can be seen that the lower pH value has a significant inhibitory effect on the growth of Lactobacillus plantarum P_17. Between 3.0 and 6.0, the density of the bacteria increases rapidly with the increase of the pH value, and the bacterial population reaches the maximum value when the pH value is 6.0. , the pH value continue...

Embodiment 3

[0089] Example 3 Lactobacillus plantarum P_17 simulated artificial gastrointestinal digestion in vitro

[0090] (1) Tolerance study on artificial gastric juice

[0091] Probiotics must pass through the harsh environment of the stomach to reach the gut in order to be effective. The food stays in the human gastric juice environment for 1 to 3 hours, and the pH value of the human gastric juice is about 3.0 on average. Therefore, probiotics must have the ability to withstand gastric juice in order to perform their functions in the human body. After the lactic acid bacteria were exposed to the artificial gastric juice with a pH value of 3.0 for 3 hours, the changes in the number of viable bacteria and the survival rate are shown in Table 7.

[0092] Table 7 The tolerance test result of lactic acid bacteria to artificial gastric juice

[0093]

[0094] It can be seen from Table 7 that after the Lactobacillus plantarum P_17 was exposed to artificial gastric juice with a pH valu...

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Abstract

The invention discloses lactobacillus plantarum P _ 17 and a method for preparing fermented apple juice by using the lactobacillus plantarum P _ 17. According to the method, apple juice is used as a raw material, and lactobacillus plantarum P _ 17 with the preservation number of CGMCC NO.19251 is used for fermentation, so that the product is prepared. The lactobacillus plantarum P _ 17 has outstanding acid resistance, sugar resistance and low temperature resistance, has strong pepsase resistance and acid resistance, can resist an artificial intestinal juice stress environment, and has bile salt resistance and high osmotic pressure resistance. The fermented apple juice is uniform and consistent in color and luster and uniform and fine in texture, and has unique aroma and apple aroma of fermentation. The viable count of the fermented apple juice prepared by the method is higher than that of other bacteria, and the gamma-aminobutyric acid, the total acid and the antioxidant value are obviously improved compared with those of common apple juice. The method is simple in production process and rich and easily available in raw materials, and has far-reaching significance in industrial production of the fruit and vegetable juice lactic acid fermented beverage.

Description

technical field [0001] The invention relates to the technical field of fruit juice fermentation, in particular to a plant lactobacillus P_17 and a method for preparing fermented apple juice by using the plant lactobacillus P_17. Background technique [0002] Apple, belonging to the Rosaceae apple plant, is the most widely planted and most productive fruit. It tastes sweet and sour, and is rich in nutrition. According to determination, every hectogram apple contains 6.5-11.2g of fructose, 2.5-3.5g of glucose, and 1.0-5.2g of sucrose. Apple can not only regulate gastrointestinal function, lower cholesterol, lower blood pressure, prevent cancer, lose weight, but also enhance children's memory. Apples not only contain a variety of vitamins, lipids, minerals, sugars and other nutrients necessary for the brain, but also contain fine fibers that are beneficial to children's growth and development and zinc that can enhance children's memory. [0003] Fermented fruit and vegetable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L2/52C12N1/20C12R1/25
CPCA23L2/382A23L2/52A23V2002/00C12R2001/25C12N1/205A23V2200/15A23V2200/30A23V2400/169
Inventor 田洪涛李晨张波张娜牛志华赵丽娜
Owner HEBEI AGRICULTURAL UNIV.
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