Hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and salmonella detection capability and preparation method thereof

A technology of fish skin gelatin and hydroxytyrosol, which is applied in the field of food science, can solve the problems of complex and cumbersome inspection procedures, complex biochemical reaction types, time-consuming and labor-intensive problems, and achieve significant economic benefits, simple and easy operation steps, and improved mechanical properties. Effect

Inactive Publication Date: 2020-06-16
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Salmonella is a Gram-negative short bacillus with blunt ends. There are many types of bacteria and various types of biochemical reactions. The conventional inspection procedures are complicated, time-consuming and labor-intensive. Detection method is the core issue of Salmonella detection research for many years

Method used

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  • Hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and salmonella detection capability and preparation method thereof
  • Hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and salmonella detection capability and preparation method thereof
  • Hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and salmonella detection capability and preparation method thereof

Examples

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Effect test

Embodiment 1

[0037] The present embodiment adopts 706g of olive leaves and 235g of sturgeon skin as starting materials, and processes them through the preparation method of the hydroxytyrosol sturgeon skin gelatin film of the present invention, comprising the following steps:

[0038] (1) Preparation of hydroxytyrosol: Add 706g of olive leaf powder to 3530mL of 0.2mol / L hydrochloric acid solution, place it in a constant temperature water bath at 80°C, take it out after 4 hours of reaction, and dissolve it in 2mol / L sodium hydroxide And to neutrality, collect the filtrate after centrifugal filtration, the concentrated solution obtained after the filtrate is concentrated is diluted with 4 times of warm water and purified through the macroporous resin, the eluent is 45% ethanol, the flow rate is 1.5BV, and the eluent is rotary evaporated After concentration, add 4 times the amount of methanol, recrystallize at 60°C to obtain the finished product of hydroxytyrosol, dry under reduced pressure to...

Embodiment 2

[0042] Commercially available hydroxytyrosol and sturgeon skin gelatin were used as raw materials. Add 8mL of neutral red indicator solution into 500mL of water, add 1mol / L NaHCO after it changes color 3Solution until the pH is 7.5, add 60g of fish skin gelatin into the solution and stir evenly, cover with a layer of plastic wrap and let it stand for 3 hours, after fully swelling, add 25g of hydroxytyrosol, 16mL of glycerin and 2mL of propylene glycol, continue stirring at 50°C for 30min, wait After fully dissolving, use double-layer filter paper to suction filter, and the obtained filtrate is treated with ultrasonic defoaming, and spread on a clean plexiglass plate (15 × 15 cm 2 ), spread it evenly, and dry it in a ventilated oven at 25°C and 50±5% relative humidity (RH) for 24h. The dried film samples were manually peeled off and stored in a desiccator to obtain the hydroxytyrosol sturgeon skin gelatin film.

Embodiment 3

[0044] Commercially available hydroxytyrosol and sturgeon skin gelatin were used as raw materials. Add 7mL of neutral red indicator solution into 500mL of water, add 1mol / L NaHCO after it changes color 3 Solution to pH 8.5, add 45g of fish skin gelatin obtained in step (2) into the solution and stir evenly, cover with a layer of plastic wrap and let stand for 4 hours, add 20g of hydroxytyrosol, 15mL of glycerin and 1.5mL of propylene glycol after fully swelling, 65°C Continue to stir for 30 min under the hood, and after fully dissolving, filter with double-layer filter paper. After ultrasonic defoaming treatment, the filtrate is spread on a clean plexiglass plate (15 × 15 cm 2 ), spread it evenly, and dry it in a ventilated oven at 25°C and 50±5% relative humidity (RH) for 24h. The dried film samples were manually peeled off and stored in a desiccator to obtain the hydroxytyrosol sturgeon skin gelatin film.

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Abstract

The invention discloses a hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and salmonella detection capability and a preparation method of the hydroxytyrosol sturgeon skin gelatin film. According to the method, sturgeon skin waste is used for preparing fish skin gelatin, then the fish skin gelatin is mixed with hydroxytyrosol to be processed into the hydroxytyrosol sturgeon skingelatin film, and the purposes of environmental protection and full utilization of resources are achieved. More importantly, propylene glycol and neutral red are added into a culture medium based onthe principle of a propylene glycol decomposition acid production test, salmonella can decompose propylene glycol to produce acid, a neutral red indicator becomes red, salmonella pollution is preliminarily detected, and accuracy and sensitivity are high. The hydroxytyrosol sturgeon skin gelatin film can be used as a food packaging material.

Description

technical field [0001] The invention relates to a hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and salmonella detection ability and a preparation method thereof, belonging to the field of food science and technology. Background technique [0002] With the concept of sustainable development, environmental protection and effective use of resources have become hot topics in today's society. The packaging safety of food and medicine has also attracted people's attention. The use of non-toxic and degradable green packaging materials has become the field of food packaging materials. research hotspots. Gelatin is obtained by the hydrolysis of collagen, has good film-forming ability and gas barrier properties, and has the advantages of anti-oxidation, biocompatibility and biodegradability, and can be used as an ideal packaging film material. At the same time, the development of the aquatic product processing industry has led to an increasing number of waste m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/06C08K5/13C08K5/053C08J5/18G01N21/78
CPCC08J5/18G01N21/78C08J2389/06C08K5/13C08K5/053Y02A50/30C07K14/78C07K14/461C12Q1/10Y02W90/10C12Q1/04C12Q2304/20
Inventor 梁承远辛亮乔乖萍李京忆武康雄李菡田斌
Owner SHAANXI UNIV OF SCI & TECH
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