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Method for improving saline-alkali tolerance of corn seedlings and improving physical and chemical properties of rhizosphere soil of corn seedlings

A technology of rhizosphere soil and salt-alkali tolerance, applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc., can solve problems that are unfavorable to sustainable agricultural development, safety disputes, and serious chemical residues problems such as avoiding residue and enrichment, improving saline-alkali tolerance, invertase activity and catalase activity

Active Publication Date: 2020-06-19
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, people have used chemical regulation to improve the salt-alkali tolerance of corn seedlings. This method has serious chemical residues, which deteriorates the soil environment and is not conducive to the sustainable development of agriculture.
In recent years, with the development of molecular biology, the research on improving the salt-alkali tolerance of maize seedlings through molecular breeding technology has gradually increased, but some molecular breeding methods, such as transgenic technology, are controversial about their safety, and their prospects need to be investigated

Method used

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  • Method for improving saline-alkali tolerance of corn seedlings and improving physical and chemical properties of rhizosphere soil of corn seedlings
  • Method for improving saline-alkali tolerance of corn seedlings and improving physical and chemical properties of rhizosphere soil of corn seedlings
  • Method for improving saline-alkali tolerance of corn seedlings and improving physical and chemical properties of rhizosphere soil of corn seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Klebsiella xenobiotics are used to improve the salt-alkali tolerance of corn seedlings and improve the physical and chemical characteristics of corn seedling rhizosphere soil

[0059] 1. Preparation of bacterial suspension

[0060] Inoculate Klebsiella xenophila in LB liquid medium, cultivate at 30°C and 180rpm until a bacterial suspension containing sufficient bacteria is obtained, and inoculate the bacterial suspension at 6000r·min -1 Centrifuge for 15min, discard the supernatant after centrifugation, wash the precipitate with sterile distilled water and resuspend, repeat 3 times and prepare 1×10 8 cfu / ml bacterial suspension, and then diluted to 1×10 according to the experimental needs 2 cfu / ml, 1×10 4 cfu / ml, 1×10 6 cfu / ml, for use.

[0061] Second, corn germination treatment

[0062] Screen Xianyu 335 corn seeds, select corn seeds with the same size and no damage on the surface, soak them in sodium hypochlorite (concentration 10%) for 10 minutes, an...

Embodiment 2

[0078] Example 2. Klebsiella and Bacillus licheniformis in combination are used to improve the salt-alkali tolerance of corn seedlings and improve the physical and chemical properties of corn seedling rhizosphere soil

[0079] 1. Preparation of bacterial suspension

[0080] Inoculate Klebsiella xenophila in LB liquid medium, cultivate at 30°C and 180rpm until a bacterial suspension containing sufficient bacteria is obtained, and inoculate the bacterial suspension at 6000r·min -1 Centrifuge for 15min, discard the supernatant after centrifugation, wash the precipitate with sterile distilled water and resuspend, repeat 3 times and prepare 1×10 6 cfu / ml bacterial suspension.

[0081] Inoculate Bacillus licheniformis in LB liquid medium, cultivate at 30°C and 180rpm until a bacterial suspension containing sufficient bacteria is obtained, and incubate the bacterial suspension at 6000r·min -1 Centrifuge for 15min, discard the supernatant after centrifugation, wash the precipitate w...

Embodiment 3

[0102] Embodiment 3, field experiment

[0103] 1. Experimental location

[0104] Heilongjiang Bayi Agricultural University Experimental Practice Base, the soil of the experimental site is saline-alkali soil (pH8.9, Na + Content 0.891g·kg -1 ).

[0105] 2. Experimental time

[0106] A total of two batches of experiments were carried out.

[0107] The first batch of sowing time: May 25, 2018, harvest time: October 6, 2018.

[0108] The second batch of sowing time: May 20, 2019, harvest time: October 3, 2019.

[0109] 3. Experimental method

[0110] 1. Carry out conventional continuous field operations such as rotary tillage and stubble removal, post-turning harrowing, fertilization, ridging, and suppression on the plot before sowing.

[0111] 2. Operate according to the following groups:

[0112] Control group (CK): Sow Xianyu 335 corn seeds, add 150ml of water to the soil when sowing, and then cover 200g of soil above the sowing place.

[0113] Experimental group (T1):...

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Abstract

The invention discloses a method for improving saline-alkali tolerance of corn seedlings and improving physical and chemical properties of rhizosphere soil of the corn seedlings. The application of Klebsiella oxytoca in any one of the following (a1)-(a6) is as follows: (a1) promoting corn growth, (a2) promoting corn growth in a saline-alkali environment, (a3) improving the enzymatic activity of corn rhizosphere soil, (a4) improving the enzymatic activity of corn rhizosphere soil in a saline-alkali environment, (a5) improving the saline-alkali tolerance of the corn, and (a6) improving the saline-alkaline tolerance of the corn by improving the enzymatic activity of the rhizosphere soil of the corn in the saline-alkaline environment. According to the invention, exogenous beneficial microorganisms are used in a corn seedling cultivation process, the method improves the saline-alkali tolerance of the corn seedlings, avoids the residue and enrichment of chemical components, meets the development requirements of green agriculture, is high in safety, is suitable for popularization and transformation, and has important significance for improving the root system micro-ecological environmentand promoting the growth of the corn seedlings under saline-alkali stress.

Description

technical field [0001] The invention relates to a method for improving the salt-alkali tolerance ability of corn seedlings and improving the physical and chemical characteristics of rhizosphere soil of corn seedlings. Background technique [0002] my country's saline-alkali land area is huge, which seriously limits the development of planting industry. Maize, as a crop sensitive to saline-alkali components, is also deeply affected. How to improve the rhizosphere soil microenvironment of maize, especially maize seedling stage, and improve the saline-alkali tolerance of maize seedlings will be of great significance to the sustainable development of agriculture in saline-alkaline areas. For a long time, people have used chemical regulation methods to improve the salt-alkali tolerance of corn seedlings. This method has serious chemical residues, which deteriorates the soil environment and is not conducive to the sustainable development of agriculture. In recent years, with the ...

Claims

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Application Information

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IPC IPC(8): A01N63/20A01N63/22A01P21/00A01G22/20A01G7/06
CPCA01G7/06A01N63/00A01G22/20
Inventor 杨丽娟杨克军
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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