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Bionic antibacterial high-adhesion dual-network hydrogel and preparation method and application thereof

A double-network, hydrogel technology, applied in bandages, medical science, etc., can solve skin wounds, inappropriate problems, etc., and achieve the effects of improved antibacterial properties, controllable speed, and strong toughness

Active Publication Date: 2020-06-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, autologous or allogeneic skin was usually used for transplantation. The former transplanted skin from other parts of the body to the wound, which would cause new skin wounds, so it was not suitable for skin grafting of large-scale trauma; the latter used other people's skin for transplantation. Transplantation, there are also some problems, including immune response and ethical issues

Method used

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  • Bionic antibacterial high-adhesion dual-network hydrogel and preparation method and application thereof
  • Bionic antibacterial high-adhesion dual-network hydrogel and preparation method and application thereof
  • Bionic antibacterial high-adhesion dual-network hydrogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Preparation of oxidized mucopolysaccharide:

[0056] Dissolve hyaluronic acid with a molecular weight of 10KDa in ddH 2 O to form a 1.0 wt% hyaluronic acid aqueous solution, and then add 1% w / v NaIO 4 Add the aqueous solution into the hyaluronic acid aqueous solution, the molar ratio of sodium periodate to hyaluronic acid is 0.1:1, stir and react at 60°C for 2h, then add 1.5mL ethylene glycol, and continue stirring for 1h to terminate the reaction. The reaction was protected from light throughout. The reaction liquid was used for dialysis purification, and the dialysis time was 7 days, and then the dialysate was freeze-dried, and the lyophilization time was 5 days.

[0057] (2) Preparation of polylysine modified by dopa:

[0058] Add polylysine with a molecular weight of 2KDa to ddH 2 O forms a 1.0% w / v aqueous solution of polylysine, dopa dissolved in ddH 2 O forms 1.5% w / v dopa aqueous solution, at 0~4 ℃, adds NHS to the dopa aqueous solution, the molar ratio...

Embodiment 2

[0062] (1) Preparation of oxidized mucopolysaccharide:

[0063] Cellulose with a molecular weight of 100KDa was dissolved in ddH 2 O to form a 0.1 wt% cellulose aqueous solution, and then 5% w / v NaIO 4 The aqueous solution was added to the cellulose aqueous solution, the molar ratio of sodium periodate to cellulose was 1:1, stirred and reacted at 30°C for 5h, then 1.5mL of ethylene glycol was added, and stirred for 1h to terminate the reaction. The reaction was protected from light throughout. The reaction solution was used for dialysis purification, and the dialysis time was 7 days, and then the dialysate was freeze-dried, and the lyophilization time was 1 day.

[0064] (2) Preparation of polylysine modified by dopa:

[0065] Add polylysine with a molecular weight of 3KDa to ddH 2 O forms a 2% w / v aqueous solution of polylysine, dopa dissolved in ddH 2 O forms a 10% w / v dopa aqueous solution, at 0-4°C, add NHS to the dopa aqueous solution, the molar ratio of NHS to dopa ...

Embodiment 3

[0069] (1) Preparation of oxidized mucopolysaccharide:

[0070] Chondroitin sulfate with a molecular weight of 2000KDa was dissolved in ddH 2 O to form 2wt% chondroitin sulfate aqueous solution, and then 10% w / v NaIO 4 The aqueous solution was added to the chondroitin sulfate aqueous solution, the molar ratio of sodium periodate to chondroitin sulfate was 2:1, stirred and reacted at 4°C for 3h, then 1.5mL of ethylene glycol was added, and stirred for 1h to terminate the reaction. The reaction was protected from light throughout. The reaction solution was used for dialysis purification, and the dialysis time was 7 days, and then the dialysis solution was freeze-dried, and the lyophilization time was 3 days.

[0071] (2) Preparation of polylysine modified by dopa:

[0072] Add polylysine with a molecular weight of 4KDa to ddH 2 O forms a 5% w / v aqueous solution of polylysine, dopa dissolved in ddH 2 O forms 0.1% w / v dopa aqueous solution, at 0~4 ℃, adds NHS to the dopa aque...

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Abstract

The invention provides bionic antibacterial high-adhesion dual-network hydrogel and a preparation method and application thereof. The preparation method comprises the steps that oxidized and modifiedmucopolysaccharide, catechol modified polyamino acid, hydrogen peroxide and horseradish peroxidase are mixed in an aqueous solution and then left to stand, and the dual-network hydrogel is obtained inone step. The gel preparation method is simple, and the raw materials are cheap and easily available. The hydrogel is composed of double networks, the first-layer network is formed by cross-linking modified mucopolysaccharide and polyamino acid, and the second-layer network is formed by enzymatic cross-linking of catechol groups. The gel is high in forming speed and controllable in forming speedand has good adhesion performance and antibacterial performance. In addition, the gel also has good cell compatibility and biocompatibility. Mucopolysaccharide in the gel system can also promote tissue healing and increase the tissue regeneration rate. The hydrogel is a gel material with a good application prospect in the field of tissue engineering skin.

Description

technical field [0001] The invention belongs to the field of biological tissue engineering, and in particular relates to a bionic antibacterial and high-adhesion double network hydrogel for skin repair and its preparation method and application. Background technique [0002] The skin is the largest organ of the human body and a barrier between the human body and the external environment. It not only undertakes functions such as protecting the body, perspiration, and perception, but also maintains the stability of the internal environment of the body. For some minor skin injuries, the skin can heal and recover by itself; but when the skin suffers a large area of ​​serious damage or a wound infection occurs, it is not realistic for the human body to recover by itself. At this time, the damaged skin of the patient needs to be treated. In the past, autologous or allogeneic skin was usually used for transplantation. The former transplanted skin from other parts of the body to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L77/02C08L5/08C08L1/02C08L5/04A61L26/00
CPCC08J3/075A61L26/0023A61L26/0019A61L26/0004A61L26/008A61L26/0085A61L26/0061A61L26/009C08J2377/02C08J2405/08C08J2301/02C08J2405/04C08L1/02C08L5/04C08L5/08C08L77/02Y02A50/30
Inventor 迟波刘帅刘鑫
Owner NANJING UNIV OF TECH
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