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Pig GATB gene as molecular marker pertinent to mortality rate of piggies, and application of pig GATB gene

A mortality and piglet technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems to be studied, achieve high sensitivity, shorten the generation interval, and improve the efficiency and accuracy of seed selection Effect

Active Publication Date: 2020-06-19
广西扬翔股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, current SNP markers for piglet mortality remain to be studied

Method used

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  • Pig GATB gene as molecular marker pertinent to mortality rate of piggies, and application of pig GATB gene
  • Pig GATB gene as molecular marker pertinent to mortality rate of piggies, and application of pig GATB gene
  • Pig GATB gene as molecular marker pertinent to mortality rate of piggies, and application of pig GATB gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In this example, refer to figure 1 , Screening molecular markers associated with piglet mortality by the following methods.

[0040] 1. Pig data collection

[0041] (1) Phenotypic data collection

[0042] The pigs used in the present invention to record phenotype data are 6073 healthy sows from Guangxi Yangxiang Co., Ltd., and the breeds include Large White, Landrace and Duroc. The pedigree information used in this study included the pedigree information of these 6073 sows, involving 35313 individuals. Data include total litter size, total deaths, stillbirths and mummies. Among them, the total number of deaths is equal to the number of stillbirths plus the number of mummies. The mortality traits studied in the present invention are the percentage of the total number of deaths in the total number of litters.

[0043] (2) Genetic data collection

[0044] Pig ear tissue was used to extract DNA, and all samples were stored in a -20°C freezer until then. Using the kit...

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Abstract

The invention provides an SNP marker pertinent to the mortality rate of piggies. The SNP marker is selected from 404th-site nucleotide of a nucleotide sequence as shown in SEQ ID NO:1, and is a base Cor a base T. Through the molecular marker, differences of mortality rate characters, between group pigs having different genotypes, can be accurately predicted, accurate and efficient selective breeding of good pig varieties being excellent in sow reproductive capacity can be realized, and the SNP marker has prominent advantages of being simple, quick, high in sensitivity, good in specificity andthe like. In pig breeding work, the molecular marker can be used as a reliable marker for mortality rate characters, and early selection is convenient to perform, so that generation intervals can beshortened, the selection intensity is enhanced, and breeding selection efficiency and accuracy can be improved.

Description

technical field [0001] The present invention relates to SNP markers and their applications. Specifically, the present invention relates to porcine GATB gene as a molecular marker related to piglet mortality and its application. More specifically, the present invention relates to SNP markers associated with piglet mortality and applications thereof. Background technique [0002] With the improvement of the living standard of Chinese residents, people's demand for meat food is gradually increasing. Driven by the growth of people's demand for meat, my country's aquaculture industry has grown rapidly. According to the white paper "China's Food Security" released on October 14, 2019, the per capita consumption of pork, beef, and mutton in China was 46.8 kg in 2018, an increase of 16.6 kg or 55% over 1996. Among the various meat products provided by the aquaculture industry in my country, pork is the largest meat consumer product, accounting for about 63% of the meat consumed. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 朱猛进赵书红安美景李新云项韬刘小磊马云龙余梅刘向东赵云翔李家连管石胜
Owner 广西扬翔股份有限公司