Unlock instant, AI-driven research and patent intelligence for your innovation.

A snap molecular marker method for identifying the color of cassava flesh

A molecular marker, cassava technology, applied in the field of molecular biology, can solve the problems of limited practicability, high price, difficult to distinguish, etc., and achieve the effects of improving practicability and efficiency, simple method, and easy-to-read strips

Active Publication Date: 2020-11-13
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of the CAPS molecular marker requires some enzyme digestion steps, which is time-consuming and labor-intensive compared with simple molecular markers.
At the same time, the enzymes required for enzyme digestion by CAPS molecular markers are expensive
In addition, in the results of the developed CAPS molecular marker detection, the yellow meat and white meat molecular marker bands are only 22 bp apart, and the bands are too close to be distinguished on the agarose gel electrophoresis map
The above reasons lead to the drawbacks of the CAPS molecular marker, which also limits its practicability

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A snap molecular marker method for identifying the color of cassava flesh

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] 1. Experimental materials:

[0030] 29 cassava leaves from self-crossing of the main cassava variety "Xinxuan 048" were selected and planted in the Lijian base of Guangxi Academy of Agricultural Sciences. Concrete 29 cassava leaves correspond to the cassava root flesh color as shown in the table below:

[0031] Table 1 Sample root flesh color

[0032] leaf sample root tuber flesh color leaf sample root tuber flesh color leaf sample root tuber flesh color 1 light yellow 11 yellow 21 light yellow 2 White 12 White 22 light yellow 3 White 13 light yellow 23 light yellow 4 White 14 light yellow 24 light yellow 5 White 15 yellow 25 light yellow 6 White 16 light yellow 26 light yellow 7 White 17 light yellow 27 light yellow 8 White 18 light yellow 28 light yellow 9 yellow 19 light yellow 29 light yellow 10 yellow 20 light yellow

[0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a SNAP molecular marker method for identifying the color of cassava flesh, belonging to the field of molecular biology. The marking method comprises the following steps: S1. adopt the CTAB method to extract cassava genomic DNA from cassava leaves, design primer pair 1 and primer pair 2 according to the DNA sequence of the PSY2 gene, and use the primer pair 1 and primer pair 2 to pair the described The cassava genomic DNA is amplified by PCR to obtain a PCR amplified product; S2. The PCR amplified product in step S1 is subjected to electrophoresis separation, and then the cassava meat color is judged according to the electrophoresis result. The method is simple, efficient, with easy-to-read bands and low price, and can greatly improve the practicability and high efficiency of molecular marker-assisted selection in cassava breeding.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a SNAP molecular marker method for identifying the color of cassava flesh. Background technique [0002] Cassava (Manihot Esculenta Crantz) is a plant of the genus Cassava (Manihot) in the family Euphorbiaceae. , the third largest food crop in tropical regions and the sixth largest food crop in the world, known as the "King of Starch". Cassava is versatile and can be used in starch production, industrial use and food consumption, especially in some African countries. Depending on the type of carotenoids in the cassava root, the color of the cassava root flesh can vary from white to pink, pale yellow and yellow. The white-fleshed cassava root is important for the starch industry, but the carotenoid-rich yellow-fleshed root is a key factor in reducing vitamin A deficiency for those who consume it directly as a staple food. At the same time, carotenoids in cassava are also import...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 尚小红严华兵陈新肖亮朴朴森曹升谢向誉王颖曾文丹陆柳英赖大欣
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI