Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of an Arabidopsis mapkkk kinase in breeding

An Arabidopsis thaliana and kinase technology, which is applied in the application field of Arabidopsis thaliana MAPKKK kinase in breeding, can solve the problem that the impact needs to be further studied, and achieve the effect of saving time and accurate seed selection.

Active Publication Date: 2021-05-11
SHANDONG AGRICULTURAL UNIVERSITY
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the advantages of Arabidopsis in genetic manipulation, it is widely used in the study of various processes of plant life activities, and a series of important discoveries have been made; but for the study of Arabidopsis protein kinase and its effect on the growth of Arabidopsis The impact still needs to be further studied and applied to the cultivation of Arabidopsis to provide a theoretical basis for future breeding work

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of an Arabidopsis mapkkk kinase in breeding
  • Application of an Arabidopsis mapkkk kinase in breeding
  • Application of an Arabidopsis mapkkk kinase in breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This embodiment 1 provides a kind of application of Arabidopsis thaliana MAPKKK kinase in breeding; wherein, Arabidopsis thaliana 47 gene is MAPKKK kinase, and its two alternative splicing forms have two phenotypes of drought resistance and early flowering respectively; There are four spliced ​​forms of 47 genes (such as figure 1 shown), named AS1, AS2, AS3 and AS4; the nucleotide sequence of AS1 is SEQ ID NO.1, the nucleotide sequence of AS2 is SEQ ID NO.2, and the nucleotide sequence of AS3 is SEQ ID NO .3, the nucleotide sequence of AS4 is SEQ ID NO.4; the amino acid sequence of the protein encoded by AS1 is SEQ ID NO.5, the amino acid sequence of the protein encoded by AS2 and AS4 is SEQ ID NO.6, the amino acid sequence of the protein encoded by AS3 The sequence is SEQ ID NO.6, and its three proteins are located in the nucleus and cytoplasm through GFP; the drought resistance phenotype experiment shows as follows: image 3 As shown, the 47-gene full deletion mutant...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of Arabidopsis thaliana MAPKKK kinase in breeding and relates to the technical field of agricultural breeding. The breeding includes creating drought-resistant germplasm and early-flowering germplasm of cruciferous plants through transgenic technology. The cruciferous plants include canola and cauliflower. The MAPKKK kinase has four spliced ​​forms, named AS1, AS2, AS3 and AS4; the AS1 overexpression plant AS1-OE and the AS3-deleted mutant CRISPRAS3-2 all exhibit a non-drought-resistant phenotype; AS3- Both the OE plants and the AS1-deleted mutant CRISPRAS1-1 showed a drought-resistant phenotype; the AS1-OE plants and AS2 / 4-OE plants showed an early flowering phenotype, and the AS3-OE plants showed a late flowering phenotype. The above research can provide a theoretical basis for the transgenic breeding of cruciferous plants.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to the application of Arabidopsis MAPKKK kinase in breeding. Background technique [0002] Arabidopsis belongs to Brassicaceae, Angiosperm Phylum, Dicotyledon Class. Biennial herb, 7-40 cm tall. Basal leaves are rosette-shaped with petioles, obovate or spoon-shaped leaves; cauline leaves are sessile, lanceolate or linear. Raceme terminal, with 4 petals, white. The advantages of Arabidopsis are small plants, short generation time, many seeds, and strong vitality. The Arabidopsis genome is 1 / 80 of the currently known plant genome, which makes it relatively easy to clone its related genes. [0003] Arabidopsis research includes forward genetics and reverse genetics. Forward genetics follows a mindset from mutant phenotype analysis to gene function understanding, focusing first on mutants with certain defects. For example, if you want to study the gene regulation pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/12C12N15/82A01H5/00A01H5/02A01H6/20
CPCC12N9/12C12N15/827C12N15/8273C12Y207/11025
Inventor 刘鹰高陈沫先武茹茹朱福远
Owner SHANDONG AGRICULTURAL UNIVERSITY