Hydrogel for cell culture and biomedical applications
A hydrogel and hard gel technology, applied in the direction of cell culture support/coating, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of not finding and searching
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[0068] The preparation method of gellan gum solution comprises dissolving gellan gum in a water-based solution with a solid content of about 0.001% w / v to 10% (w / v), heating the solution to about 100°C to about 150°C, preferably From about 100°C to about 121°C, at a pressure of about 1 psi to about 40 psi, preferably at a pressure of about 1 psi to about 30 psi, for a period of time of about 3 to about 30 minutes, preferably a period of about 5 to about 20 minutes within the paragraph. This preparation method is also applicable to the preparation of biomolecule-modified gellan gum solutions. Such biomolecules are selected from the group consisting of: cells, peptides, proteins, lipids, polysaccharides, growth factors, growth hormones, antibodies, enzymes, cell receptors, cell ligands, antibiotics, antimicrobials, antifungals, antifungals, Albumin, serum, including RGD, IKVAV, REDV, YIGSRY, polylysine with NH 2 , COOH and CONH 2 Group of functional peptide molecules. Aqueou...
Embodiment 1
[0080] Embodiment 1 (as Figure 4 shown): 5×10 in DMEM medium 5 cells / mL to prepare a βTC3 cell suspension.
[0081] Prepare a 1% w / v solution of gellan gum. The gellan gum solution was mixed with the cell suspension at a ratio (v / v) of 4:1. Gelation started immediately after mixing, showing an increase in G'. By showing G' > 50 Pa, a soft hydrogel can be formed within 15 min. βTC3 cells were suspended in hydrogels. Afterwards, adding more DMEM medium on top of the soft gel will further stabilize the hydrogel by showing an increase in G'. After 2 hours (or overnight) a hard gel will form showing G' above 500Pa. βTC3 cells can grow in hydrogels and form 3D colonies after 3 days.
Embodiment 2
[0082] Embodiment 2 (as Figure 4 shown): 5×10 in RPMI medium 5 cells / mL to prepare Ins-1 cell suspension.
[0083] Prepare a 1% w / v solution of gellan gum. The gellan gum solution was mixed with the cell suspension at a ratio of 1:1 (v / v). Gelation started immediately after mixing, showing an increase in G'. By showing G' > 50 Pa, a soft hydrogel can be formed within 15 min. Ins-1 cells are suspended in hydrogels. Afterwards, adding more RPMI medium on top of the soft gel, the hydrogel will be further stabilized by showing increased G'. After 2 hours (or overnight) a hard gel will form showing G' above 300Pa. Ins-1 cells can grow in hydrogels and form 3D colonies after 3 days.
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