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Use of a material based on carbon nanotubes and phycoerythrin

A technology of phycoerythrin and carbon nanotubes, which can be applied to wave energy or particle radiation treatment materials, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. The blue protein structure is not very stable and other problems, to achieve the effect of improving stability, good stability, and improving light conversion efficiency

Active Publication Date: 2022-03-22
TAIZHOU BINMEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the structure of phycocyanin is not very stable, and the photosensitization effect is weak, so its application as a photosensitizer is greatly limited.

Method used

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  • Use of a material based on carbon nanotubes and phycoerythrin
  • Use of a material based on carbon nanotubes and phycoerythrin
  • Use of a material based on carbon nanotubes and phycoerythrin

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1 A method for preparing a material based on carbon nanotube-coupled phycoerythrin, comprising the following steps:

[0032] (1) Prepare a carboxylated single-walled carbon nanotube dispersion with a concentration of 1 mg / mL with deionized water, take 1 mL of the carboxylated single-walled carbon nanotube dispersion, dilute it 50 times, take 10 mL, and ultrasonicate for 90 minutes;

[0033] (2) Add 0.2 mL of 9 mg / mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) solution and 0.2 mL of 5 mg / mL dissolved in PBS buffer to the above dispersion mL of N-hydroxysulfosuccinimide (NHS) solution dissolved in PBS buffer (with the addition of EDC and specific weight ratios of NHS to carboxylated SWNTs of 9:1 and 5:1), ultrasonically dispersed 30min and placed at room temperature (25°C) under magnetic stirring for 18h; the PBS buffer is 50mM sodium phosphate buffer with a pH of 7.0;

[0034] (3) Then add 4 mg of phycoerythrin to the above reaction solution, the spec...

Embodiment 2

[0036] Embodiment 2 A method for preparing a material based on carbon nanotube-coupled phycoerythrin, comprising the following steps:

[0037] (1) Prepare a carboxylated single-walled carbon nanotube dispersion with a concentration of 1 mg / mL with deionized water, take 1 mL of the carboxylated single-walled carbon nanotube dispersion, dilute it 20 times, take 10 mL, and ultrasonicate for 90 minutes;

[0038] (2) Add 0.5mL 9mg / mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) solution and 0.5mL 5mg / mL dispersed in PBS buffer to the above dispersion mL of N-hydroxysulfosuccinimide (NHS) solution dispersed in PBS buffer (the specific weight ratios of EDC and NHS to single-walled carbon nanotubes were 9:1 and 5:1), ultrasonically dispersed for 30 min and Stir magnetically at room temperature (25° C.) for 18 hours; the PBS buffer is 50 mM sodium phosphate buffer with a pH of 7.0;

[0039] (3) Then add 10 mg of phycoerythrin to the above reaction solution, the specific amount ...

Embodiment 3

[0041] Embodiment 3 A method for preparing a material based on carbon nanotube-coupled phycoerythrin, comprising the following steps:

[0042] (1) Prepare a carboxylated single-walled carbon nanotube dispersion with a concentration of 1mg / mL with deionized water, take 1mL of the carboxylated single-walled carbon nanotube dispersion, dilute it 10 times, take 10mL, and first sonicate for 90min;

[0043] (2) Add 1mL 9mg / mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) solution and 1mL 9mg / mL dissolved in PBS buffer to the above dispersion N-hydroxysulfosuccinimide (NHS) solution in PBS buffer (the specific weight ratios of EDC and NHS to single-walled carbon nanotubes are 9:1 and 5:1), ultrasonically dispersed for 30min and placed in Magnetic stirring at room temperature (25° C.) for 18 h; the PBS buffer is 50 mM sodium phosphate buffer with a pH of 7.0;

[0044] (3) Then add 15 mg of phycoerythrin to the above reaction solution, the specific amount of addition is 15 mg of p...

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Abstract

The invention discloses the use of a novel material based on carbon nanotubes and phycoerythrin, that is, as a photosensitizer for phototherapy to treat tumors. It can not only overcome the chemical inertia of single-walled carbon nanotubes and improve their light conversion efficiency, but also improve the stability of phycoerythrin after the two are connected, and the effect is more durable. The new material not only has good stability, but also has excellent cancer treatment effect as a photosensitizer for phototherapy and cancer treatment with both photothermal effects.

Description

technical field [0001] The invention belongs to the technical field of new materials, and in particular relates to the application of a material based on carbon nanotubes and phycoerythrin. Background technique [0002] Single-walled carbon nanotubes can be considered as the extension of fullerene molecules in a cage structure in one dimension, or as a closed tubular structure formed by curling graphite layers. Drugs related to single-walled carbon nanotubes must first pass through the human circulatory and metabolic systems before they can reach the designated tissue sites. Due to its chemical inertness, it is difficult to interact with other molecules, so the modified single-walled carbon nanotubes can be used as carriers to couple and transport various biomolecules, such as drugs, proteins, DNA and RNA, etc., and they enter cells through endocytosis , better results can be obtained. At the same time, as an excellent photothermal material, carbon nanotubes have strong ab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/55A61P35/00
CPCA61K41/0052A61K41/0057A61K47/55A61P35/00A61K2300/00
Inventor 赵华伟蒲洋杜若冉邓琳陈帅钰李文军
Owner TAIZHOU BINMEI BIOTECH
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