Full-humanized antagonism antibody screened from ScFv phage antibody library by using THY-1/CD90 as target point

A phage antibody library, THY-1 technology, applied in the field of antagonistic antibodies, to achieve the effect of rapid screening of antibodies and low price

Active Publication Date: 2020-06-23
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Despite the above reports, there has been no literature on the screening of inhibitor antibodies targeting THY-1 from the ScFv phage antibody library using phage display and other technologies

Method used

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  • Full-humanized antagonism antibody screened from ScFv phage antibody library by using THY-1/CD90 as target point
  • Full-humanized antagonism antibody screened from ScFv phage antibody library by using THY-1/CD90 as target point
  • Full-humanized antagonism antibody screened from ScFv phage antibody library by using THY-1/CD90 as target point

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Embodiment Construction

[0025] see figure 1 and figure 2 , the present invention discloses a fully human antagonistic antibody selected from the ScFv phage antibody library with THY-1 / CD90 as the target, and the fully human antagonistic antibody with THY-1 / CD90 as the target is screened from the ScFv phage antibody library The fully human antagonistic antibody is a single-chain antibody, the DNA sequence of the fully human antagonistic antibody is shown in the 1E01 DNA sequence, and the amino acid sequence is shown in the 1EO1 amino acid sequence.

[0026] As a preference: the E01 DNA sequence is used to encode the 1E01 amino acid sequence.

[0027] As a preference: determine the DNA sequence and amino acid sequence of the fully human antagonistic antibody by sequencing, and determine the DNA sequence and amino acid sequence of the fully human antagonistic antibody respectively, so that the obtained fully human antagonistic antibody has targeting THY-1 / Specificity of CD90.

[0028] As a preferen...

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Abstract

The invention discloses a full-humanized antagonism antibody screened from an ScFv phage antibody library by using THY-1 / CD90 as a target point. The full-humanized antagonism antibody screened from anScFv phage antibody library by using THY-1 / CD90 as a target point is a single-chain antibody, the DNA sequence of the full-humanized antagonism antibody is shown as 1E01DNA sequence, and the amino acid sequence is shown as 1EO1 amino acid sequence. According to the technical scheme, the full-humanized antagonism antibody is screened from human ScFv phage antibody library by using THY-1 / CD90 as the target point, and the DNA sequence and the amino acid sequence are respectively determined. The full-humanized antagonism antibody obtained by the method has specificity targeting THY-1 / CD90. The generated antibody can overcome the defects of the antibody generated by a hybridoma technique, the purpose of being high in flux, and quickly screening the antibody can also be realized, and the full-humanized antagonism antibody is low in cost.

Description

technical field [0001] The present invention relates to the technical field of antagonistic antibodies, in particular to a fully human antagonistic antibody selected from the ScFv phage antibody library with THY-1 / CD90 as the target. Background technique [0002] THY-1 (thymocyte antigen-1), also known as CD90, is a highly conserved glycoprotein. At present, it is found on the surface of nerve cells, thymocytes, fibroblast subsets, endothelial cells, mesangial cells, and hematopoietic stem cells. THY-1 regulates cell-cell and cell-cytoplasmic responses and plays an important role in nerve regeneration and metastasis, inflammation, and fibrosis. Lee reported that THY-1 is a novel marker of inflammatory cytokines promoting angiogenesis; Bradley JE and Rege TA two research groups respectively reported that THY-1 is closely related to angiogenesis in some diseases. [0003] In recent years, some literatures have reported that the use of phage display technology from the human ...

Claims

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Application Information

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IPC IPC(8): C07K16/28C12N15/13G01N33/569
CPCC07K16/2803G01N33/56983C07K2317/24C07K2317/622
Inventor叶辉向敏胡煊煊褚晓颖李美琪汪子晗万乐予桑侃如杜邦
OwnerWENZHOU MEDICAL UNIV