Protein quantitative kit based on bioinformatics analysis

Abstract

The invention discloses a protein quantitative kit based on bioinformatics analysis, and relates to the technical field of protein quantitative kits. The ket comprises a box body. The box body is movably provided with a liquid storage plate. A fixed plate is movably mounted at the front end of the box body. The fixed plate is sequentially overlapped with a sample pad, a combination pad and an absorption pad. A fluorescent microsphere reagent marked by a protein monoclonal antibody is injected into the combination pad. The preparation method of the protein monoclonal antibody labeled fluorescent microsphere reagent comprises the following steps: preparing a protein monoclonal antibody labeled fluorescent microsphere reagent; putting the boric acid buffer solution into a storage container; putting the fluorescent microspheres into a storage container. According to the device, the solution concentration of a protein monoclonal antibody labeled fluorescent microsphere reagent is increasedby increasing the concentration of a fluorescent microsphere solution. The mixed solution can be effectively and fully filtered through the ceramic membrane, so that the mixed solution is purified more thoroughly, the filtering efficiency is improved, the preparation time of the reagent is shortened, and the working intensity of external personnel is reduced.

Description

technical field [0001] The invention relates to the technical field of protein quantification kits, in particular to a protein quantification kit based on bioinformatics analysis. Background technique [0002] The kit is used for containing chemical reagents for detection of chemical components, drug residues, virus types and other chemical reagents. Generally used by hospitals and pharmaceutical companies, bioinformatics is a science that uses computers as tools to store, retrieve and analyze biological information in the research of life sciences. It is one of the major frontier fields of life sciences and natural sciences today. It will also be one of the core fields of natural science in the 21st century. Its research focus is mainly reflected in two aspects of genomics (Genomics) and proteomics (Proteomics). Specifically, it starts from nucleic acid and protein sequences and analyzes the structures expressed in the sequences. Functional biological information, when bio...

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Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a protein quantification kit based on bioinformatics analysis. The traditional preparation method internally uses fluorescent microspheres and boric acid buffer to shake and mix. The structure of this mixing method is too simple. And the mixing effect is not good, and the mixing time is long, which will seriously consume the working time of the construction personnel. Moreover, in the process of filtering the centrifuged fluorescent microsphere activation solution to remove the sediment, because there is no better filtering mechanism, the filtering time is too long. Long, the time consumed is too long, which affects work efficiency and delays the preparation progress

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