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Subunit h protein of Peste des petits ruminants virus and its preparation method and application

A PPR, subunit technology, applied in the fields of botanical equipment and methods, antisense single-stranded RNA viruses, biochemical equipment and methods, etc. The problem of high protein and production cost, to achieve stable and efficient secretion expression, less virus amount, and solve the effect of high production cost

Active Publication Date: 2022-02-11
NOVO BIOTECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, subunit vaccines also have obvious shortcomings: high production costs and limited applications
[0007] However, when using engineered cells to express H protein, if the coding gene sequence of H protein is not optimized, the engineered cells basically do not express H protein, and it is difficult to obtain H protein with excellent immunogenicity and stability for small ruminant Prevention and control of zooepidemic virus

Method used

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  • Subunit h protein of Peste des petits ruminants virus and its preparation method and application
  • Subunit h protein of Peste des petits ruminants virus and its preparation method and application
  • Subunit h protein of Peste des petits ruminants virus and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1a: Structural analysis and optimization of the H protein of Peste des petits ruminants virus was carried out to construct the coding gene sequence of the subunit H protein of Peste des petits ruminants virus.

[0054] By analyzing the H protein sequence of Peste des petits ruminants virus (GenBank: KM091959.1), it was found that the genome sequence was 7332-9661bp, encoding the H protein sequence of Peste des petits ruminants. Further analysis 1M-35P is the intracellular region of H protein, 36Y-58I is the transmembrane region of H protein, and 59R-609V is the extracellular region of H protein. Combined with the experience of research on the expression of viral envelope proteins, the extracellular region of the H protein expressed by CHO-K1 cells was selected as the immunogenic protein of the present invention, that is, the amino acid sequence of 59R-609V, and the amino acid sequence is a known fragment, which can be obtained by Obtained by NBCI inquiry.

[0055] Howe...

Embodiment 2

[0244] Example 2: The difference from Example 1 is that in this example, codon optimization is not performed on the coding gene sequence of the subunit H protein, that is, the coding gene sequence of the subunit H protein is shown in SEQ ID NO.2 .

Embodiment 3

[0245] Example 3: The difference from Example 1 is that in this example, codon optimization is performed on the coding gene sequence of the subunit H protein, and its specific coding gene sequence is shown in SEQ ID NO.4.

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PUM

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Abstract

The invention belongs to the technical field of animal vaccines and veterinary biological products, and in particular relates to a subunit H protein of Peste des petits ruminants virus and its preparation method and application. The subunit H protein is a truncated head of the H protein of Peste des petits ruminants virus The amino acid sequence of the functional region protein and subunit H protein is: ① the amino acid sequence shown in the sequence SEQ ID NO.1; ② the immunogenicity obtained from the sequence SEQ ID NO.1 through substitution, deletion or addition of several amino acids The derived amino acid sequence, and the derived amino acid sequence has 80%-100% sequence identity with the sequence SEQ ID NO.1. Subunit H protein is mainly prepared by constructing recombinant plasmids, transfecting cell lines with recombinant plasmids, screening highly expressed cell lines, and purifying the subunit H protein of Peste des petits ruminants virus, which can be better applied to subunits of Peste des petits ruminants virus. Among unit vaccines or diagnostic reagents, it has the characteristics of efficient secretion and expression, high protein purity, easy purification, reduced production cost, and high safety performance.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines and veterinary biological products, and in particular relates to a subunit H protein of Peste des Petits Ruminants virus and its preparation method and application. Background technique [0002] Peste des Petits of Ruminats (PPR) is a highly contagious viral disease caused by Peste des Petits of Ruminats Virus (PPRV). The World Organization for Animal Health (OIE) classifies it as a Class A infectious disease, and my country also classifies it as a Class I epidemic disease. PPRV mainly infects sheep, especially goats are highly susceptible. Once infected sheep will be characterized by sudden fever, depression, oral ulcers, cough, eye and nose discharge, and diarrhea. The morbidity and mortality can reach 100%, causing huge economic losses and seriously affecting the development of the sheep industry. [0003] At present, there is no effective treatment for the disease, mainly relying on ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/115C12N15/45C12N15/85A61K39/155A61P31/14
CPCC07K14/005C12N15/85A61K39/12A61P31/14C12N2760/18422C12N2760/18434C12N2800/107C12N2800/22A61K2039/552
Inventor 钱泓吴有强卞广林张强徐玉兰吴素芳车影
Owner NOVO BIOTECH CORP
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