Subunit h protein of Peste des petits ruminants virus and its preparation method and application
A PPR, subunit technology, applied in the fields of botanical equipment and methods, antisense single-stranded RNA viruses, biochemical equipment and methods, etc. The problem of high protein and production cost, to achieve stable and efficient secretion expression, less virus amount, and solve the effect of high production cost
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Embodiment 1
[0053] 1a: Structural analysis and optimization of the H protein of Peste des petits ruminants virus was carried out to construct the coding gene sequence of the subunit H protein of Peste des petits ruminants virus.
[0054] By analyzing the H protein sequence of Peste des petits ruminants virus (GenBank: KM091959.1), it was found that the genome sequence was 7332-9661bp, encoding the H protein sequence of Peste des petits ruminants. Further analysis 1M-35P is the intracellular region of H protein, 36Y-58I is the transmembrane region of H protein, and 59R-609V is the extracellular region of H protein. Combined with the experience of research on the expression of viral envelope proteins, the extracellular region of the H protein expressed by CHO-K1 cells was selected as the immunogenic protein of the present invention, that is, the amino acid sequence of 59R-609V, and the amino acid sequence is a known fragment, which can be obtained by Obtained by NBCI inquiry.
[0055] Howe...
Embodiment 2
[0244] Example 2: The difference from Example 1 is that in this example, codon optimization is not performed on the coding gene sequence of the subunit H protein, that is, the coding gene sequence of the subunit H protein is shown in SEQ ID NO.2 .
Embodiment 3
[0245] Example 3: The difference from Example 1 is that in this example, codon optimization is performed on the coding gene sequence of the subunit H protein, and its specific coding gene sequence is shown in SEQ ID NO.4.
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