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Novel animal perfusion method

An animal, perfusion technology, applied in the fields of medical science, veterinary instruments, veterinary surgery, etc., to achieve the effect of less interference

Inactive Publication Date: 2020-07-10
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite some progress in other studies, there are no reports of orthotopic perfusion models in mice: a) bypassing clearing organs such as liver, spleen, and kidneys; b) including pelvic organs, skin, muscles, reproductive organs (in males) , bladder, prostate and bone marrow

Method used

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Embodiment Construction

[0031] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0032] A novel animal perfusion method, comprising the steps of:

[0033] 1. Preheating the perfusion system

[0034] Prepare the perfusion system before surgery by starting a water bath that circulates all jacketed components, make sure the tubing is clean and replace if necessary; to limit the velocity of the perfusate, use a wet chamber bubble trap as perfusion liquid chamber;

[0035] 2. Vascular Catheterization

[0036] 2.1: Use an anesthesia machine to induce anesthesia in mice at an oxygen flow rate of 0.3 L / min;

[0037] 2.2; Pre-wet 2 sutures with a needle in double distilled water; preheat 2 sutures with distilled water;

[0038] 2.3; Lay the rat under anesthesia on its back on the polystyrene foam board, with the head facing the operator; wipe the abdomen with isopropanol, and use scissors to cut the abdominal skin in a "Y...

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PUM

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Abstract

The invention discloses a novel animal perfusion method. The method comprises the following steps: preheating a perfusion system; preparing the perfusion system by starting circulating water baths ofall components provided with a water jacket before an operation, ensuring that pipelines are clean, and replacing the pipelines when necessary; limiting the speed of perfusion liquid; performing vascular catheter insertion operation; and arranging the perfusion system. A model provided by the invention is a very useful tool for researching pathological processes and drug delivery mechanisms, migration / metastasis of circulating tumor cells into tumors, and interaction between an immune system and perfusion organs and tissues; interference caused by metabolic tissues such as liver, kidney, spleen and the like on experiments can be effectively avoided; and moreover, the invention provides a brand-new research approach with smaller interference for drug research and development, so that drug and tumor researchers can more intuitively know the action modes and effects of drugs on tumor tissues.

Description

technical field [0001] The invention relates to the field of isolated organ research, specifically a new animal perfusion method. Background technique [0002] Ex vivo perfusion is an important physiological tool for studying the function of isolated organs such as liver and kidney. Meanwhile, long-term perfusion settings for bone, bladder, skin, prostate, and reproductive organs have not been described. Here, we report for the first time an in situ lower body perfusion protocol that bypasses the major clearing organs (kidney, liver, and spleen). The circuit is established by inserting the abdominal aorta and inferior vena cava and cauterizing (ligating) the surrounding vessels. The perfusion medium was Ringer's lactate with 5% BSA (bovine serum). Perfusion is performed by a peristaltic pump, and blood flow and body temperature are maintained for up to 3 hours. In situ staining for fluorescent lectin and Hoechst confirmed successful perfusion of the microvasculature. ...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/04
CPCA61D1/00A61D7/04
Inventor 陈芳芳任平
Owner JILIN UNIV
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