Rapid quantitative extracellular vesicle sample concentration method

Abstract

The invention relates to a rapid quantitative extracellular vesicle sample concentration method which comprises the following steps: 1, a sieve plate divides the space in a concentration container into two parts which are partially communicated with each other and are respectively communicated with the outside, one end is an upper opening, and the other end is a lower opening; 2, a to-be-concentrated sample is added from the upper opening to one side of the sieve plate, and fills the space on one side of the lower opening; 3, a super absorbent material is added into the container from the upper opening; 4, a continuous force is applied to the to-be-concentrated sample from one side of the upper opening to one side of the lower opening until the flowable liquid on one side of the absorbentmaterial of the sieve plate is completely absorbed; and 5, discharged residual liquid is collected. The concentration speed is high, the concentration from 20ml to 1ml only needs 3-5min, and the rapidwater absorption capacity of the super absorbent material is fully utilized in the concentration process. The excessive super absorbent material can accelerate water absorption, and due to the fact that the super absorbent material is low in price, the using cost caused by excessive use is limited.

Description

technical field [0001] The invention belongs to the technical field of construction, in particular to a method for rapidly quantitatively concentrating extracellular vesicle samples. Background technique [0002] Biomacromolecules often need to be concentrated during the preparation process for preservation and identification purposes. [0003] Commonly used enrichment methods: [0004] 1. Evaporation and concentration under reduced pressure and heating: [0005] The boiling point of the liquid is lowered by lowering the liquid surface pressure. The higher the vacuum degree of decompression, the lower the boiling point of the liquid and the faster the evaporation. This method is suitable for the concentration of some heat-labile biomacromolecules. [0006] Disadvantages: Not suitable for thermolabile extracellular vesicles. [0007] 2. Air flow evaporation and concentration: [0008] The flow of air can accelerate the evaporation of the liquid, spread the solution into a...

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Problems solved by technology

[0006] Cons: Not suitable for thermolabile extracellular vesicles

[0009] Disadvantages: This method is slow to concentrate and is not suitable for the concentration of a large number of solutions

[0012] Disadvantages: The concentration of the concentrate is restricted by the eutectic concentration and the degree of separation of ice crystals, and the cost is high

[0015] Disadvantages: Use ultrafiltration membrane or dialysis membrane to wrap and wait for concentration, the concentration speed is slow, and the concentration amount is not easy to control

[0018] Disadvantages: high cost of use, production technology is monopolized by foreign companies

[0019] Compared with other concentration methods, the cost of ultrafiltration material is low in the existing absorption concentration method. One method is to put the superabsorbent material directly into the sample to be concentrated; the disadvantages are: 1) the concentration end point is not easy to control, 2) it is difficult to concentrate to a fixed Volume; 3) Excessive superabsorbent material will lead to over-concentration and loss of samples, 4) After concentration, the water-absorbent material and the concentrated solution are not easy to separate; 5) The concentration speed of the solution containing salt is slow;

[0020] Another way is to wrap the superabsorbent material in the ultrafiltration membrane bag; its disadvantages are: 1) due to the low water flux of the ultrafiltration membrane under no pressure conditions, the concentration process is slow, and the superabsorbent material cannot be fully utilized. The performance of fast water absorption, usually it takes more than 1 hour to use superabsorbent materials to saturate and absorb water to concentrate. 2) The concentration rate is limited, generally only 3-4 times concentrated. , thus limiting the concentration

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