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Method for monitoring 16 mycotoxins in rice

A mycotoxin, rice technology, applied in the direction of microorganism-based methods, fungi, biochemical equipment and methods, etc., to achieve the effect of reducing workload, easy operation, and simple steps

Inactive Publication Date: 2020-07-10
昆明市疾病预防控制中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a method for monitoring 16 kinds of mycotoxins in rice, which solves the problem of directly using liquid chromatography-mass spectrometry to detect rice

Method used

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Embodiment Construction

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0024] The embodiment of the present invention provides the monitoring method of 16 kinds of mycotoxins in rice, including the following contents:

[0025] S1 Weigh 10-15g rice samples from different batches of rice species, pass the rice samples through a grinding device to make rice slurry, and control the grinding temperature at 20-40°C to prevent fungi from losing their activity in high-temperature environments. The amount of distilled water is 5 to 10 times that of the rice sample, an...

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Abstract

The invention provides a method for monitoring 16 mycotoxins in rice, and relates to the field of mycotoxin detection. The method for monitoring the 16 mycotoxins in the rice comprises the following steps: sampling, separating, culturing and proliferating fungi, observing the fungi, determining the types of the fungi, and finally quantitatively measuring the content of the mycotoxins in the rice by adopting liquid chromatography-mass spectrometry. Whether fungi for toxin generation exist in the rice is determined through pre-culture, if yes, the content of mycotoxin is quantitatively detectedaccording to the types of the fungi, and if not, detection does not need to be conducted again, so that the rice batch using the liquid chromatography-mass spectrometry detection mode is greatly reduced, the cost is saved, and meanwhile the culture detection mode is simple in step and easy to operate.

Description

technical field [0001] The invention relates to the technical field of mycotoxin detection, in particular to a method for monitoring 16 kinds of mycotoxins in rice. Background technique [0002] Mycotoxins are metabolites produced by fungi growing in food or feed that are harmful to humans and animals. Aflatoxin B is present in rice 1 , Aflatoxin B 2 , Aflatoxin G 1 , Aflatoxin G 1 , Nivalenol, Deoxynivalenol, 3-Acetyldeoxynivalenol, Zearalenone, Ochratoxin A, Fumonisin B 1 , Fumonisin B 2 , Fumonisin B 3 , T-2 toxin, HT-2 toxin, Aspergillus versicolor and other toxins. Rice needs to be tested to avoid food safety problems. [0003] Most of the existing detection methods of rice mycotoxins are directly detected by liquid chromatography-mass spectrometry. Chromatographs and mass spectrometers have a large workload, slow inspection speed, and high maintenance costs for chromatographs and mass spectrometers. Contents of the invention [0004] (1) Solved technical pr...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N33/10C12N1/14C12R1/645
CPCG01N30/02G01N33/10C12N1/14
Inventor 李文廷蒋孟圆李洁申颖陈俊秀张瑞雨梁志坚李旭
Owner 昆明市疾病预防控制中心
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