Biofilm observation objective table of laser confocal microscope

Abstract

The invention discloses a biofilm observation objective table of a laser confocal microscope. The biofilm observation objective table comprises an objective table assembly and a biofilm attachment sheet bearing assembly; the objective table assembly comprises two sliding strips which are arranged in parallel in a spaced mode. The biofilm attachment sheet bearing assembly comprises two moving tables which are arranged in parallel in a spaced mode. The two ends of the moving table are connected with the two sliding strips in a sliding mode respectively, an adjusting block capable of moving up and down is arranged below each moving table, and the bottoms of the opposite side faces of the two adjusting blocks extend outwards by a certain distance to form a supporting table used for containingbiofilm attachment sheets. The biofilm observation objective table is simple in structure, easy to assemble and convenient to operate, can solve the problems of fixing and supporting of the biofilm attachment sheets during observation of the laser confocal microscope in the experiment process, and can also be applied to other electron microscope platforms such as an inverted microscope, a common optical microscope and a fluorescence microscope.

Description

technical field [0001] The invention belongs to the field of laser confocal electron microscope accessories, in particular to a laser confocal electron microscope biofilm observation stage. Background technique [0002] Biofilm (Biofilm) refers to the group of microorganisms attached to the surface of biological or non-biological materials, which are wrapped by extracellular macromolecules secreted by themselves and have certain three-dimensional structural characteristics. Studies have shown that both bacteria and fungi can form biofilms, and biofilms The basic components include: water, macromolecular polymers, adsorbed nutrients, microorganisms themselves and metabolites, etc. At the same time, the formation process of biofilm includes the initial colonization stage, irreversible adhesion stage, structural differentiation stage, development and maturity There are experimental data confirming that each of the above processes will be affected by many factors, such as the ma...

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Problems solved by technology

[0003] In order to better control and utilize various biofilms, scientists all over the world have conducted a lot of research on the formation process of biofilms, gene expression, influencing factors, metabolic regulation, and drug resistance mechanisms. During the process, it is often necessary to use various electron microscopes (such as scanning electron microscopes, transmission electron microscopes, fluorescence microscopes and laser confocal scanning microscopes, etc.) , and the in vitro attachment carriers of biofilms are usually glass slides (such as laboratory slides and coverslips), iron sheets and plastic sheets, etc. Usually, these attached carriers such as coverslips are either too thin , or it is too small, and the stage configured by the electron microscope itself is often unable to carry and fix them. If these biofilm attachment sheets are placed in (or on) a container, due to an additional physical barrier between the sample and the objective lens, It is easy to cause focusing difficulties. Therefore, it is urgent to invent a stage capable of carrying biofilm attachment sheets, thereby improving the convenience and accuracy of biofilm electron microscope observation (especially laser confocal scanning microscope)

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