Field environment nitrogen fixation activity ARA determination method and device

Abstract

The invention discloses a field environment nitrogen fixation activity ARA determination method and device. The field environment nitrogen fixation activity ARA determination method comprises the following steps: S1, selecting biological soil crusts (hereinafter referred to as biological crusts) with nitrogen fixation activity under a field environment condition; s2, spraying distilled water to the biological crust, and then covering the biological crust with a glass cover to form a closed space; S3, injecting acetylene gas into the glass cover by using an acetylene injection device, and thenculturing for more than 3 hours; S4, after the culture is finished, extracting the gas in the glass cover by using an extraction device, then analyzing the gas by using a gas chromatographic analysisinstrument, and finally performing measurement to obtain data. A field in-situ observation and research mode is adopted; the field in-situ measurement result is obtained by matching with a self-designed simple and convenient scientific research instrument, the measurement result better conforms to the actual situation of the field environment, and the problems that an existing ARA measurement method can only perform measurement in a laboratory and the measurement result cannot accurately reflect the actual situation of field complex environment change are solved.

Description

technical field [0001] The invention relates to the technical field of measuring nitrogen-fixing activity ARA, in particular to a method and device for measuring nitrogen-fixing activity ARA in a field environment. Background technique [0002] Nitrogen (N) availability is generally considered to limit ecological processes in terrestrial ecosystems, and explicit consideration of nitrogen biogeochemical processes, including biological nitrogen fixation, is critical for understanding ecosystem responses to environmental change. In terrestrial ecosystems, free-living nitrogen fixation processes that occur on a variety of substrates are almost ubiquitous and often represent a major route to bioavailable nitrogen sources, yet their importance is often underestimated. Nitrogen to nitrogen (N 2 ) in the atmosphere in a form that is not available to vascular plants. N 2 It must first be "fixed" or "reduced" by eukaryotes such as eubacteria and cyanobacteria to ammonia (NH + ) fo...

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Problems solved by technology

[0005] The purpose of the invention of the present invention is to: aim at the above-mentioned existing problems, provide field environment nitrogen fixation activity ARA measurement method and device, through the mode of field in situ observation research, cooperate self-designed simple scientific research instrument, utilize this scientific research instrument in the field environment Study the nitrogen fixation activity of soil nitrogen-fixing microorganisms in response to hydrothermal conditions and complex and variable environmental factors, and then can obtain accurate field in situ measurement results, which are more in line with the actual situation in the field environment, and solve the problem that the existing ARA measurement methods can only be used in Determination in the laboratory, and the measurement results cannot accurately reflect the actual situation of complex environmental changes in the field

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