Microbial inoculum combination applied to livestock and poultry manure fermentation, and preparation method and use method thereof

A technology for livestock and poultry manure and fermentation bacteria agent, which is applied in the field of livestock and poultry manure fermentation bacteria agent combination and its preparation, can solve the problems of poor pertinence and low efficiency, and achieves strong pertinence, good effect, and is not easy to infect miscellaneous bacteria. Effect

Active Publication Date: 2020-07-17
BEIJING BEINONG ENTERPRISE MANAGEMENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are not strong pertin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A preparation method applied to livestock and poultry manure fermentation bacterial agent, comprising the steps of:

[0056] (1) Activation and rejuvenation of bacteria:

[0057] ① Strains: Lactobacillus Brucella, Bacillus licheniformis BNQ-5, Lactobacillus plantarum BNQ-32, Candida utilis;

[0058] ② Basal medium: glucose 20g / L, peptone 10g / L, beef extract 10g / L, dipotassium hydrogen phosphate 2g / L, diammonium citrate 2g / L, sodium acetate 5g / L, Tween 80 1g / L, Magnesium sulfate 0.60g / L, manganese sulfate 0.25g / L, pH 6.2-6.4, 121°C, sterilize for 20min;

[0059] ③ Culture and activate Lactobacillus Brucella, Bacillus licheniformis BNQ-5, Lactobacillus plantarum BNQ-32, and Candida utilis, and culture them at 35°C for 24 hours to make a compound fermentation liquid.

[0060] (2) Bacterial liquid expansion culture:

[0061] ①Synergistic culture medium: Add 10% chicken manure dry powder, 10% pig manure dry powder, 10% sheep manure dry powder, and 10% cow manure dry powde...

Embodiment 2

[0072] A preparation method applied to livestock and poultry manure fermentation bacterial agent, comprising the steps of:

[0073] (1) Activation and rejuvenation of compound bacteria: same as Example 1

[0074] (2) Bacterial liquid expansion culture:

[0075] ①Synergistic culture medium: add 15% chicken manure dry powder, 15% pig manure dry powder, 15% sheep manure dry powder, and 15% cow manure dry powder to the basic medium components in step (1), corresponding to four kinds Synergistic culture solution: No. 1 culture solution, No. 2 culture solution, No. 3 culture solution, and No. 4 culture solution;

[0076] ②Amplified fermentation process: add the compound fermentation bacteria liquid in step (1) to culture medium No. Ⅰ, Ⅱ, Ⅲ and Ⅳ respectively according to the ratio of 1:20, and the others are the same as in Example 1;

[0077] (3) centrifugal treatment: with embodiment 1;

[0078] (4) Addition of protective agent and adsorption material: same as Example 1;

[007...

Embodiment 3

[0087] Example 3—Comparison of 1, 2, and 3 experiments in Example 10 shows that the compost moisture (55%-65%), bacterial agent addition (1.25±0.1:1000) and the auxiliary materials within the scope of the claims The selection can achieve the expected fermentation effect. Therefore, it can be considered that the experimental results of the three groups are parallel.

[0088] Example 3:

[0089] Experimental location: Southern pig farm, ambient temperature 22°C±4

[0090] 6 tons of pig manure, with a water content of 80.5%, were divided into 6 piles, each pile was 1 ton, numbered 1, 2, 3, 4, 5, and 6 piles;

[0091] Groups 1, 2, and 3 are experimental groups for inventing bacterial agents. Add chopped straw and sawdust mixture to piles 1, 2, and 3 of pig manure, so that the C / N ratio of the three piles of pig manure is 20:1, and the water content is 55%, 60% and 65%; then according to the total compost mass of 1.15:1000, 1.25:1000, 1.35:1000 respectively add No. Ⅱ bacterial a...

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Abstract

The invention relates to a microbial inoculum combination applied to livestock and poultry manure fermentation, and a preparation method and use method thereof, and belongs to the field of composting.According to the invention, four mixed probiotics comprising lactobacillus buchneri, bacillus licheniformis BNQ-5, lactobacillus plantarum BNQ-32 and candida utilis are used to perform mixing culturein four enhancing culture solutions to obtain a No.I microbial inoculum, a No.II microbial inoculum, a No.III microbial inoculum and a No.IV microbial inoculums respectively special for chicken manure, pig manure, sheep manure and cow manure. In a preparation process, through selection of a novel protective agent and selection of an optimal bacterial liquid adsorption material, compost of the microbial inocula is quick in temperature rise, and the decomposition effect is good, so that the microbial inocula are not only suitable for warm weather in the south, but also suitable for compost fermentation in cold weather in the north. The invention is helpful for farmers to select more targeted fermentation microbial inocula, and rapid fermentation and decomposition of livestock manure are realized.

Description

Technical field: [0001] The invention relates to the technical field of microbial inoculations, in particular to a combination of inoculations for fermenting livestock and poultry manure and methods for its preparation and use. Background technique: [0002] With the development of animal husbandry, the mode of livestock and poultry breeding has gradually shifted to large-scale and intensive farming. And livestock and poultry feces are produced in large quantities and are becoming a new major source of pollution. Untreated feces directly enter the farmland, which not only seriously affects the environment, produces toxic odors and greenhouse gases, but also carries various pathogenic microorganisms and insect eggs, which seriously threatens environmental safety. Statistics show that the annual production of livestock and poultry manure in my country is about 3.8 billion tons. As my country's environmental management becomes more and more standardized, this new source of pol...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C05F11/08C05F17/20C05F15/00C12R1/225C12R1/10C12R1/25C12R1/72
CPCC05F3/00C05F11/08C05F17/00C12N1/16C12N1/20C05F5/002C05F9/04C05F11/00C05F11/02Y02W30/40
Inventor 乔富强乔琳李冬青金忠辉王辉焦春昱李志敏
Owner BEIJING BEINONG ENTERPRISE MANAGEMENT
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