An improved process for producing outer membrane vesicles

An outer membrane vesicle, physiological technology, applied in the field of medical microbiology and vaccinology, can solve the problems of cell death bacteria, lysis and so on
CN111433348APending Publication Date: 2020-07-17INTRAVACC有限责任公司
8 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INTRAVACC有限责任公司
Publication Date
2020-07-17

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention relates to the fields of medical microbiology and vaccines. In particular the invention relates to a process wherein the spontaneous release of bacterial outer membrane vesicles(OMV) of Gram-negative bacteria is stimulated by application of a dissolved oxygen tension (DOT) that is higher than a physiological DOT. The thus produced OMVs are for use in vaccines. The inventionfurther relates to OMV obtainable by said process, and to a pharmaceutical composition comprising such OMV. The present invention further relates to the use of OMV of the present invention as a medicament in particular for use in a method for eliciting an immune response.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the fields of medical microbiology and vaccinology. In particular, the present invention relates to a method for producing Gram-negative bacterial spontaneous outer membrane vesicles (OMVs) for use in vaccines, OMVs obtainable by said method, and pharmaceutical compositions comprising such OMVs. The invention also relates to the use of an OMV according to the invention as a medicament, in particular in a method of eliciting an immune response. Background technique

[0002] Outer membrane vesicles (OMVs) are naturally produced by Gram-negative bacteria and are involved in pathogenesis, intercellular communication, and stress response (Kulp and Kuehn 2010). Recent studies have shown membrane vesicle formation in Gram-positive bacteria and archaea (Ellen et al. 2009; Rivera et al. 2010). OMVs are spherical nanoparticles, vesicles composed of a phospholipid bilayer containing proteins and lipopolysaccharide (LPS), and the vesicle...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More