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Early diagnosis and treatment biomarker for alveolar echinococcosis and application thereof

A technology of alveolar echinococcosis and targets, applied in the field of biomarkers for early diagnosis and treatment of alveolar echinococcosis, can solve problems such as difficult to effectively diagnose early alveolar echinococcosis

Pending Publication Date: 2020-07-24
QINGHAI UNIV AFFILIATED HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even with these advanced imaging techniques, it remains difficult to effectively diagnose early alveolar echinococcosis

Method used

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  • Early diagnosis and treatment biomarker for alveolar echinococcosis and application thereof
  • Early diagnosis and treatment biomarker for alveolar echinococcosis and application thereof
  • Early diagnosis and treatment biomarker for alveolar echinococcosis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, proteomics analysis

[0050] The samples used in this example were isolated liver and plasma samples from five participants clinically diagnosed with alveolar echinococcosis (AE) and five normal participants (without alveolar echinococcosis) of plasma samples. This protocol has been approved by the Research Ethics Committee of the Affiliated Hospital of Qinghai University, and informed consent has been obtained from each subject.

[0051] 1. Pathological evaluation

[0052] Isolated livers from participants with alveolar echinococcosis (AE) were divided into three groups: (1) normal tissue of the liver (Normal); (2) liver lesion tissue (Lesion); (3) lesion tissue The edge part (Edge), located between normal and diseased. Histological examination showed no alveolar Echinococcus invasion in the normal part ( figure 1 A, D), but tissue lesions were found in liver fibrous tissue ( figure 1 B, C, D).

[0053] 2. Protein expression profile in liver tissue ...

Embodiment 2

[0083] Example 2, verification of markers

[0084] The samples in this example are plasma samples from fifty-six clinically confirmed AE patients and fifty-five healthy controls (without alveolar echinococcosis). None of the subjects had a history of respiratory or cardiovascular disease, such as chronic obstructive pulmonary disease, asthma, infectious disease, congenital heart disease, or hypertensive heart disease. Informed consent has been obtained from each subject.

[0085] Plasma samples were incubated at room temperature for 20 minutes, then centrifuged at 3000 rpm for 20 minutes, the supernatant was transferred to a new tube, and the samples were stored at -80°C for further ELISA analysis.

[0086] ELISA analysis was performed using commercial kits according to the instructions.

[0087] ALDH1A1 detection kit was purchased from Shanghai Jianglai Biotechnology Co., Ltd., item number: JL19746.

[0088] TAGLN2 detection kit was purchased from Shanghai Jianglai Biotech...

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Abstract

The invention discloses an early diagnosis and treatment biomarker for alveolar echinococcosis and application thereof. The invention protects application of a substance for detecting target proteinsin preparation of a product for diagnosis or auxiliary diagnosis of alveolar echinococcosis. The target proteins include (a1) or (a2) or (a3): the (a1) comprises ALDH1A1 protein, TAGLN2 protein and FLN alpha protein; the (a2) comprises any two of ALDH1A1 protein, TAGLN2 protein and FLN alpha protein; and the (a3) comprises ALDH1A1 protein or TAGLN2 protein or FLN alpha protein. The inventors of the invention use proteome correlation analysis of liver and plasma to detect potential candidate biomarkers in AE patients. Results show that ALDH1A1, TAGLN2 and FLN alpha may be early prediction indexes of the AE patients and can be used as candidate diagnosis indexes of early AE patients.

Description

technical field [0001] The invention relates to a biomarker for early diagnosis and treatment of alveolar echinococcosis and its application. Background technique [0002] Alveolar echinococcosis is a parasitic disease caused by alveolar echinococcosis (Echinococcus multilocularis), also known as multilocular echinococcosis. The adults of Echinococcus alveolar are similar to Echinococcus granulosus, but the body is shorter (1.4-3.4mm). Unlike Echinococcus granulosus, alveolar coccoids do not form large vesicles but instead form spongy vesicles. The vesicles grow faster, the ascus is exophytic, and the number of protoscoleia is less. Alcoholic larvae mainly parasitize in the liver, occasionally in the lung and brain, and its lesions and consequences are more serious than echinococcosis granulosus. Gross lesions are generally single bulky, sometimes nodular, or both. The alveolar coccoid cysts are often composed of numerous small vesicles, such as sponge or honeycomb. The ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 庞明泉樊海宁王海久刘川川靳翔董家鸿
Owner QINGHAI UNIV AFFILIATED HOSPITAL
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