Serum-free and heterologous component-free mesenchymal stem cell culture medium and application thereof
A technology of mesenchymal stem cells and culture medium, applied in the field of mesenchymal stem cell culture medium, can solve the problems of restricting the industrial transformation and clinical application of mesenchymal stem cells, poor batch stability of culture medium, and low efficiency of adipocytes, etc. Proliferation ability, improve multi-directional differentiation function, improve the effect of immune regulation ability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0109] Embodiment 1 A kind of serum-free, xenogeneous component-free mesenchymal stem cell culture medium
[0110] A serum-free, heterogeneous-free mesenchymal stem cell culture medium, said culture medium is composed of DMEM / F12 medium (1×), human platelet lysate and human lipocalin 2, said human platelet lysate The volume content of the liquid in the mesenchymal stem cell medium is 20%, and the content of the human lipocalin 2 in the mesenchymal stem cell medium is 200 μg / mL.
[0111] The pH value of the mesenchymal stem cell culture medium is 7.38, and the osmotic pressure is 341 mOsm / kg.
Embodiment 2
[0112] Embodiment 2 A kind of serum-free, xenogeneous component-free mesenchymal stem cell culture medium
[0113] A serum-free, heterogeneous-free mesenchymal stem cell culture medium, said culture medium is composed of DMEM / F12 medium (1×), human platelet lysate and human lipocalin 2, said human platelet lysate The volume content of the liquid in the mesenchymal stem cell medium is 10%, and the content of the human lipocalin 2 in the mesenchymal stem cell medium is 100 μg / mL.
[0114] The pH value of the mesenchymal stem cell culture medium is 7.35, and the osmotic pressure is 335mOsm / kg.
Embodiment 3
[0115] Embodiment 3 A kind of serum-free, xenogeneous component-free mesenchymal stem cell culture medium
[0116] A serum-free, heterogeneous-free mesenchymal stem cell culture medium, said culture medium is composed of DMEM / F12 medium (1×), human platelet lysate and human lipocalin 2, said human platelet lysate The volume content of the liquid in the mesenchymal stem cell medium is 5%, and the content of the human lipocalin 2 in the mesenchymal stem cell medium is 50 μg / mL.
[0117] The pH value of the mesenchymal stem cell culture medium is 7.42, and the osmotic pressure is 346mOsm / kg.
PUM
Property | Measurement | Unit |
---|---|---|
Osmotic pressure | aaaaa | aaaaa |
Osmotic pressure | aaaaa | aaaaa |
Osmotic pressure | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com