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Novel coronavirus detection test kit

A coronavirus and kit technology, applied in the detection/testing of microorganisms, resistance to vector-borne diseases, DNA/RNA fragments, etc., can solve problems such as low sensitivity, false negatives, and inability to take into account internal control channels, etc., to avoid false positives Negative issues, pollution reduction effects

Pending Publication Date: 2020-07-28
PILOT GENE TECH HANGZHOU CO LTD
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing novel coronavirus diagnostic kits based on qPCR technology have the following problems: 1) the sensitivity is not high, and it is easy to cause missed detection and false negative; Take into account the internal control channel; or test two copies, that is, use two genes and one internal control gene to form the detection system

Method used

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  • Novel coronavirus detection test kit
  • Novel coronavirus detection test kit
  • Novel coronavirus detection test kit

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0035] This embodiment includes the following steps:

[0036] 1. Based on the sequence of the conserved region of the new coronavirus, use primer express software to design primer probes. The specific information is shown in Table 1 below:

[0037] Table 1

[0038]

[0039] Among them, FAM, CY5, and VIC in Table 1 are common fluorescent reporter groups, and MGB is a fluorescent quencher group.

[0040] 2. Samples

[0041] (1) Pseudovirus RNA: ORF1ab gene pseudovirus and N gene pseudovirus were purchased from a biological company. After mixing 10 μL each, RNA was extracted with an RNA extraction kit. The test was divided into 2 groups, group A was diluted 100 times, and group B 500 times; spare.

[0042] (2) Internal control gene: Genomic DNA of oral exfoliated cells was extracted with a blood / cell / tissue genomic DNA extraction kit and diluted to 5ng / μL for later use.

[0043] 3. Digital PCR detection process (preparation of digital PCR reaction solution, droplet chip g...

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Abstract

The invention belongs to the field of biology, and particularly relates to a novel coronavirus detection test kit. The invention discloses a primer probe composition. The primer probe composition comprises a nucleotide sequence group with a nucleotide sequence as shown in SEQ ID NO: 1-9. The invention also discloses a test kit comprising the primer probe composition, and a method for carrying outnovel coronavirus detection by using the primer probe composition or the test kit. According to the test kit disclosed by the invention, positive results as low as 1copy / mu L can be detected, and thediagnosis problem of the existing qPCR technology-based novel coronavirus test kit false negative during fluorescence quantification is avoided; and two detection channels and one internal control channel can be performed at the same time, so that pollution possibly caused by multiple times of sample adding is reduced.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a novel coronavirus detection kit. Background technique [0002] Coronaviruses (CoV), belonging to the subfamily Coronavirinae of the Coronaviridae family, are enveloped single-stranded positive-sense RNA viruses with the longest genome among known RNA viruses. Human coronavirus (Human Coronaviruses, hCoVs) infection is one of the important pathogens causing human respiratory diseases. The detection rate of coronaviruses in common respiratory pathogens is about 3%-10%, mainly infecting infants, the elderly and adults with low immune function , is an important pathogen that causes clinical symptoms such as cough, fever, laryngitis, bronchitis and pneumonia. [0003] On February 11, 2020, WHO Director-General Tedros Adhanom Ghebreyesus announced at the press conference of the Global Research and Innovation Forum that the disease caused by a new type of coronavirus was named "COV...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/70C12Q1/6851
CPCC12Q1/6851C12Q1/701C12Q2600/16C12Q2600/166C12Q2531/113C12Q2537/143C12Q2563/159C12Q2545/101Y02A50/30
Inventor 朱海涛李旭新夏江
Owner PILOT GENE TECH HANGZHOU CO LTD