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Stem cell scaffold for treating aneurysm and vascular stenosis based on alloy core, and preparation method of stem cell scaffold

A technology for vascular stenosis and aneurysm, which is applied in the field of stem cell scaffolds for the treatment of aneurysms and vascular stenosis and its preparation method, which can solve the problems of difficult cell colonization and achieve the effect of convenient colonization and high loading rate

Active Publication Date: 2020-07-31
北京臻溪谷医学研究中心(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult for cells to colonize the aneurysm, so there is an urgent need for a carrier material that can help stem cells fix the aneurysm

Method used

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  • Stem cell scaffold for treating aneurysm and vascular stenosis based on alloy core, and preparation method of stem cell scaffold
  • Stem cell scaffold for treating aneurysm and vascular stenosis based on alloy core, and preparation method of stem cell scaffold
  • Stem cell scaffold for treating aneurysm and vascular stenosis based on alloy core, and preparation method of stem cell scaffold

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Experimental program
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Effect test

Embodiment 1

[0033] This embodiment provides an alloy scaffold with a high cell loading rate. The alloy scaffold with a high cell loading rate is processed by the alloy metal scaffold through the following steps:

[0034] Dip the alloy metal stent into a gelatin solution with a concentration of 0.5%, let it stand at room temperature for 4 hours, and air-dry it at room temperature;

[0035] Immerse the air-dried alloy metal stent in a polylysine solution with a concentration of 0.5%, let it stand at room temperature for 1 hour, and air-dry it at room temperature;

[0036] Immerse the above air-dried alloy metal stent in a mixed solution containing fibronectin and laminin, the concentrations of both fibronectin and laminin are 0.5%, and let it stand at room temperature for 4 hours to obtain an alloy with a high cell loading rate bracket;

[0037] Among them, the alloy metal stent was purchased from Johnson & Johnson Cordis Company.

Embodiment 2

[0039] This embodiment provides an alloy scaffold with a high cell loading rate. The alloy scaffold with a high cell loading rate is processed by the alloy metal scaffold through the following steps:

[0040] Immerse the alloy metal stent in a gelatin solution with a concentration of 1%, let it stand at room temperature for 6 hours, and air-dry it at room temperature;

[0041] Immerse the air-dried alloy metal stent in a polylysine solution with a concentration of 1%, let it stand at room temperature for 2 hours, and air-dry it at room temperature;

[0042] Immerse the above air-dried alloy metal stent in a mixed solution containing fibronectin and laminin, the concentrations of both fibronectin and laminin are 1%, and let it stand at room temperature for 5 hours to obtain an alloy with a high cell loading rate bracket;

[0043] Among them, the alloy metal stent was purchased from Johnson & Johnson Cordis Company.

Embodiment 3

[0045] This embodiment provides an alloy scaffold with a high cell loading rate. The alloy scaffold with a high cell loading rate is processed by the alloy metal scaffold through the following steps:

[0046] The alloy metal stent was immersed in a gelatin solution with a concentration of 1.5%, placed overnight at 4°C, and air-dried at room temperature;

[0047] Immerse the air-dried alloy metal stent in a polylysine solution with a concentration of 1.5%, let it stand at room temperature for 3 hours, and air-dry it at room temperature;

[0048] Immerse the above air-dried alloy metal scaffold in a mixed solution containing fibronectin and laminin, the concentrations of both fibronectin and laminin are 1.5%, and place it at 4°C overnight to obtain a high cell loading rate alloy bracket;

[0049] Among them, the alloy metal stent was purchased from Johnson & Johnson Cordis Company.

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Abstract

The invention provides a porous fibrous reticular biological scaffold for treating aneurysm and vascular stenosis based on an alloy core and a preparation method of the stem cell scaffold. The preparation method of the scaffold comprises the following steps: pretreatment of an alloy metal scaffold: coating an alloy metal scaffold with gelatin, polylysine, fibronectin and laminin in sequence to prepare a pretreated scaffold; and attachment: putting the pretreated scaffold into a single-cell suspension of mesenchymal stem cells, and performing treating until the mesenchymal stem cells are attached to the scaffold, so that the porous fibrous reticular biological scaffold for treating aneurysm based on the alloy core is obtained. The porous fiber net-shaped biological scaffold for treating aneurysm and vascular stenosis based on the alloy core is high in mesenchymal stem cell loading rate, and corresponding mesenchymal stem cells can be conveniently planted to the aneurysm affected part.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a stem cell scaffold for treating aneurysm and vascular stenosis based on an alloy core and a preparation method thereof Background technique [0002] Aneurysmal subarachnoid hemorrhage (aSAH) is a disease with high morbidity and high mortality. In recent years, cell therapy has become a new strategy for the treatment of various diseases. The purpose is to replace and restore the structure and function of damaged cells . Stem cell therapy is expected to be an adjuvant treatment for patients with acute aSAH to reduce the secondary damage after cerebral hemorrhage and reduce the risk of aneurysm rebleeding in a short period of time. However, it is difficult for cells to colonize the aneurysm, so there is an urgent need for a carrier material that can help stem cells fix the aneurysm. Contents of the invention [0003] In order to solve the above technical problems, the pre...

Claims

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Application Information

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IPC IPC(8): A61L31/02A61L31/10A61L31/14A61L31/16C12N5/0775
CPCA61L31/022A61L31/10A61L31/14A61L31/146A61L31/16A61L2300/30A61L2420/08C12N5/0662C12N2500/30C12N2500/40C12N2500/44C12N2501/11C12N2531/00C12N2533/52C08L89/00C08L89/06C08L77/04
Inventor 曹毓琳滕睿頔穆士卿王颖
Owner 北京臻溪谷医学研究中心(有限合伙)
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