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Method for realizing efficient hydrogen production based on chlorella cell biomineralization

A cell biology, chlorella technology, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as high cost, inability to achieve large-scale hydrogen production, cumbersome processes, etc., and achieve time The effect of short, high stability and high hydrogen production efficiency

Active Publication Date: 2020-07-31
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention is to solve the problem that the existing biological hydrogen production process is cumbersome, the cost is high, and large-scale hydrogen production cannot be realized, and a method for realizing efficient hydrogen production based on the biomineralization of chlorella cells is provided

Method used

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  • Method for realizing efficient hydrogen production based on chlorella cell biomineralization
  • Method for realizing efficient hydrogen production based on chlorella cell biomineralization
  • Method for realizing efficient hydrogen production based on chlorella cell biomineralization

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specific Embodiment approach 1

[0021] Embodiment 1: A method for realizing efficient hydrogen production based on biomineralization of chlorella cells in this embodiment is specifically carried out according to the following steps:

[0022] 1. Using Chlorella pyrenoidosa as the substrate, use BG-11 medium in a light incubator with a temperature of 25-30°C for continuous illumination of 1200-4800LUX for cultivation. When the number of Chlorella pyrenoidosa cells reaches the logarithmic growth phase when used;

[0023] 2. Wash the chlorella pyrenoidosa cells first with sodium chloride solution, then wash with deionized water for 3 to 5 times, and collect the cleaned chlorella pyrenoidosa cells by centrifugation; take polydiallyl dimethyl Diallyl ammonium chloride was dissolved in deionized water to obtain a polydiallyl dimethyl ammonium chloride solution, which was added dropwise to the cleaned Chlorella pyrenoidosa cells, and stirred for 30 to 40 minutes at a speed of 600 to 800 rpm; Wash with deionized wat...

specific Embodiment approach 2

[0028]Embodiment 2: The difference between this embodiment and Embodiment 1 is that the concentration of the sodium chloride solution in step 2 is 0.02 mol / L. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0029] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the concentration of the polydiallyldimethylammonium chloride solution in step 2 is 4-6 mg / mL. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for realizing efficient hydrogen production based on chlorella cell biomineralization. The invention belongs to the technical field of biological energy, and particularly relates to a method for achieving efficient hydrogen production based on chlorella cell biomineralization. The invention aims to solve the problems that existing biological hydrogen production processes are complicated and high in cost, and cannot realize large-scale hydrogen production. According to the method, chlorella pyrenoidosa is taken as a basic living cell, layer-by-layer self-assembly is carried out on the surface of the chlorella pyrenoidosa based on electrostatic interaction, and finally a functional MOF material which takes GMP as a ligand and Cu<2+> as a metal center and hasnano-enzyme activity is formed. By adding an oxygen consumption substrate, the oxygen concentration of a system is quickly reduced under the catalytic action of MOF, so that efficient and quick hydrogen production is realized. The whole system is easy to construct, mild in process and environmentally friendly, meanwhile, the functionalized MOF shell layer not only serves as a catalytic effect, butalso can maintain the basic activity of cells, and the life cycle of the cells in an unfavorable environment is prolonged. The method is used for biological hydrogen production.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and in particular relates to a method for realizing efficient hydrogen production based on biomineralization of chlorella cells. Background technique [0002] With the continuous development of the economy, people's demand for energy is getting higher and higher. However, due to the excessive development and utilization of non-renewable energy sources such as coal and oil, not only the energy crisis has become increasingly serious, but also the environmental problems have become more and more prominent. Therefore, seeking a clean, green, efficient and easy-to-obtain renewable energy has attracted more and more attention of scientific and technological workers. [0003] In order to promote the sustainable development of energy, hydrogen has been favored by many researchers due to its renewability, environmental friendliness and excellent energy conversion efficiency. It is an ideal energy sourc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12N1/12B01J31/22C12R1/89
CPCC12P3/00C12N1/12B01J31/1691B01J31/2217B01J2531/16Y02P20/133
Inventor 黄鑫徐志君祁家瑞刘小曼
Owner HARBIN INST OF TECH
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