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A method for efficient hydrogen production based on biomineralization of chlorella cells

A cell biology, chlorella technology, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as high cost, inability to achieve large-scale hydrogen production, cumbersome processes, etc., and achieve time The effect of short, high stability and high hydrogen production efficiency

Active Publication Date: 2022-06-07
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention is to solve the problem that the existing biological hydrogen production process is cumbersome, the cost is high, and large-scale hydrogen production cannot be realized, and a method for realizing efficient hydrogen production based on the biomineralization of chlorella cells is provided

Method used

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  • A method for efficient hydrogen production based on biomineralization of chlorella cells
  • A method for efficient hydrogen production based on biomineralization of chlorella cells
  • A method for efficient hydrogen production based on biomineralization of chlorella cells

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specific Embodiment approach 1

[0021] Embodiment 1: A method for realizing efficient hydrogen production based on Chlorella cell biomineralization of the present embodiment is specifically carried out according to the following steps:

[0022] 1. Using Chlorella pyrenoidosa as the substrate, use BG-11 medium to cultivate in a light incubator with a temperature of 25-30 °C for 1200-4800 LUX of continuous light. When the number of Chlorella pyrenoidosa cells reaches the logarithmic growth phase time to use;

[0023] 2. Wash the Chlorella pyrenoidosa cells first with sodium chloride solution, then with deionized water for 3 to 5 times, and collect by centrifugation to obtain the cleaned Chlorella pyrenoidea cells; Dissolving ammonium chloride in deionized water to obtain a polydiallyldimethylammonium chloride solution, adding the cleaned Chlorella pyrenoidosa cells dropwise, and stirring at a speed of 600-800 rpm for 30-40 min; using Washing with deionized water for 3 to 5 times, and collecting by centrifugat...

specific Embodiment approach 2

[0028]Embodiment 2: The difference between this embodiment and Embodiment 1 is that the concentration of the sodium chloride solution described in step 2 is 0.02 mol / L. Other steps and parameters are the same as in the first embodiment.

specific Embodiment approach 3

[0029] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the concentration of the polydiallyldimethylammonium chloride solution in step 2 is 4-6 mg / mL. Other steps and parameters are the same as in the first or second embodiment.

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Abstract

A method for efficient hydrogen production based on biomineralization of chlorella cells. The invention belongs to the technical field of bioenergy, and in particular relates to a method for realizing efficient hydrogen production based on biomineralization of chlorella cells. The invention aims to solve the problems that the existing biological hydrogen production process is cumbersome, the cost is high, and large-scale hydrogen production cannot be realized. Method: In the present invention, Chlorella pyrenoidosa is used as the basic living cell, and layer-by-layer self-assembly is carried out on its surface based on electrostatic interaction, and finally a Cu cell with GMP as the ligand is formed. 2+ It is a functional MOF material with a metal center and nanoenzyme activity. By adding an oxygen-consuming substrate, under the catalysis of MOF, the oxygen concentration of the system is rapidly reduced to achieve efficient and rapid hydrogen production. The whole system is simple to construct, the process is mild, and it is green and environmentally friendly. At the same time, the functionalized MOF shell not only acts as a catalyst, but also maintains the basic activity of cells and improves the survival cycle of cells in unfavorable environments. The invention is used for biological hydrogen production.

Description

technical field [0001] The invention belongs to the technical field of biological energy, and in particular relates to a method for realizing efficient hydrogen production based on the biomineralization of chlorella cells. Background technique [0002] With the continuous development of the economy, people's demand for energy is getting higher and higher. However, due to the over-exploitation and utilization of non-renewable energy sources such as coal and oil, not only the energy crisis is becoming more and more serious, but also the environmental problems are becoming more and more prominent. Therefore, the search for a clean, green, efficient and easily available renewable energy has attracted more and more attention of scientific and technological workers. [0003] In order to promote the sustainable development of energy, hydrogen has been favored by the majority of researchers due to its renewability, environmental friendliness and excellent energy conversion efficien...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12N1/12B01J31/22C12R1/89
CPCC12P3/00C12N1/12B01J31/1691B01J31/2217B01J2531/16Y02P20/133
Inventor 黄鑫徐志君祁家瑞刘小曼
Owner HARBIN INST OF TECH
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