A kind of straw decomposing agent and its preparation method and application

A straw decomposing agent and straw technology are applied in the field of straw decomposing agents, which can solve the problems of long fermentation time and the like, and achieve the effects of good effect, fast decomposing speed and reducing diseases, insects and weeds.

Active Publication Date: 2022-07-05
德州市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing decomposing agent has the problem that the fermentation time is too long

Method used

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  • A kind of straw decomposing agent and its preparation method and application
  • A kind of straw decomposing agent and its preparation method and application
  • A kind of straw decomposing agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Preparation of Aspergillus niger agent:

[0056] (1) Inoculate the Aspergillus niger bacterial strain on the solid PDA medium plate, activate and cultivate at 37°C until the Aspergillus niger spores are covered with two-thirds of the medium plate;

[0057] (2) Scrape off the Aspergillus niger spores growing on the PDA medium plate, add sterile water to prepare the spore concentration of not less than 1.5×10 3 cfu / ml of Aspergillus niger spore suspension;

[0058] (3) The Aspergillus niger spore suspension was inoculated into the sporulation medium at a volume-to-mass ratio of 1:300, the thickness of the sporulation medium was 2 cm, and the sporulation was induced by constant temperature culture at 25°C for 7 days:

[0059] (4) pulverize the spore-forming medium together with the produced Aspergillus niger spores to 20 mesh, then freeze to -15° C., and vacuum dry to a water content of 6% to obtain an Aspergillus niger inoculum.

[0060] Preparation of Trichoderma virid...

Embodiment 2

[0081] Preparation of Aspergillus niger agent:

[0082] (1) Inoculate the Aspergillus niger bacterial strain on the solid PDA medium plate, activate and cultivate at 37°C until the Aspergillus niger spores are covered with two-thirds of the medium plate;

[0083] (2) Scrape off the Aspergillus niger spores growing on the PDA medium plate, add sterile water to prepare the spore concentration of not less than 1.5×10 3 cfu / ml of Aspergillus niger spore suspension;

[0084] (3) The Aspergillus niger spore suspension was inoculated into the sporulation medium at a volume-to-mass ratio of 1:300, the thickness of the sporulation medium was 5 cm, and the sporulation was induced by constant temperature culture at 40°C for 20 days:

[0085] (4) pulverize the sporulation medium together with the produced Aspergillus niger spores to 60 mesh, then freeze to -15° C., and vacuum dry to a water content of 7% to obtain an Aspergillus niger inoculum.

[0086] Preparation of Trichoderma viride...

Embodiment 3

[0107] Preparation of Aspergillus niger agent:

[0108] (1) Inoculate the Aspergillus niger bacterial strain on the solid PDA medium plate, activate and cultivate at 37°C until the Aspergillus niger spores are covered with two-thirds of the medium plate;

[0109] (2) Scrape off the Aspergillus niger spores growing on the PDA medium plate, add sterile water to prepare the spore concentration of not less than 1.5×10 3 cfu / ml of Aspergillus niger spore suspension;

[0110] (3) The Aspergillus niger spore suspension was inoculated into the sporulation medium at a volume-to-mass ratio of 1:300, the thickness of the sporulation medium was 3 cm, and the sporulation was induced by constant temperature culture at 30°C for 15 days:

[0111] (4) pulverize the sporulation medium together with the produced Aspergillus niger spores to 40 mesh, then freeze to -15° C., and vacuum dry to a water content of 6.5% to obtain an Aspergillus niger inoculum.

[0112] Preparation of Trichoderma viri...

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Abstract

The invention discloses a straw decomposing agent and a preparation method and application thereof, and relates to the technical field of straw decomposing agents. In preparation, Aspergillus niger, Trichoderma viride, Bacillus amyloliquefaciens and Saccharomyces cerevisiae are respectively made into inoculants, and then mixed and crushed to obtain; when the straw decomposing agent is used, it is directly mixed with water and then added with urea and sprayed evenly on the Straw surface, and then turn the straw into the ground. The straw decomposing agent of the present invention can be applied to return the straw to the field and the temperature rises rapidly, can achieve efficient decomposing of the straw within 4-5 days, can enhance the ability of the decomposing agent to antagonize crop pathogenic bacteria, and significantly inhibit the occurrence of crop fusarium wilt and yellow leaf disease .

Description

technical field [0001] The invention relates to the technical field of straw decomposing agents, in particular to a straw decomposing agent and a preparation method and application thereof. Background technique [0002] Straw returning is a method of applying the straw as a by-product of crops into the soil directly or after being piled and decomposed. Straw contains a large amount of fresh organic materials. After returning to the farmland, after a period of decomposition, it can be converted into organic matter and quick-acting nutrients, which can not only improve the physical and chemical properties of the soil, but also supply certain nutrients. Straw returning to the field can promote agricultural water saving, cost saving, increase production and efficiency, and has received sufficient attention in environmental protection and sustainable agricultural development. [0003] Returning straw to the field can increase the fresh organic matter of the soil and improve soil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/16C12N1/20C05F17/20C05F17/80C05G3/60C12R1/685C12R1/885C12R1/07C12R1/865
CPCC12N1/14C12N1/16C12N1/20C05F17/20C05F17/80C05G3/60C05C9/00C05F11/00Y02W30/40
Inventor 朱金英裴艳婷王友平张书良
Owner 德州市农业科学研究院
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